鱼肠道弧菌免疫检测方法的研究

采用灭活的鱼肠道弧菌作为抗原,制备兔抗血清,建立了鱼肠道弧菌的间接荧光抗体检测技术(indirect im-munofluorescence assay test,IFAT)、Western-blotting免疫印迹检测技术。IFAT结果显示阳性信号覆盖整个菌体,明亮,清晰,荧光信号呈长弧形且两端钝圆,与鱼肠道弧菌形状一致。Western-blotting结果显示共10条蛋白带发生免疫反应,其中6条蛋白带结果较清晰,显示出较强的特异性免疫反应,阴性对照组无条带。IFAT和Western-blotting结果说明所建立检测方法能够准确、快速的检测出鱼肠道弧菌。     

机械化直播油菜的优点及高产栽培技术

针对近年来油菜生产机械化程度低、用工多、成本高、效益低、面积逐年萎缩的情况,通过多年试验与总结,探索与完善了机械化直播油菜的高产栽培技术,实现了省工节本、提高机械化水平的目的,也为大面积油菜生产提供技术参考。     

杂交稻三香优410的特征特性及栽培技术

三香优410在建瓯种植,表现出丰产性好、抗性较强、米质优良等特性,栽培上要注意选好秧田、培育壮秧、合理密植、科学施肥、做好水管、病虫防治。     

农机与农艺结合中存在的问题及对策

农机与农艺紧密结合,对于农业经济发展具有重要意义,是未来农业发展的必然趋势。阐述农机与农艺结合的重要性,分析农机与农艺结合过程中存在的问题,提出加快农机与农艺结合的有效措施,以期为现代农业的发展提供参考。     

日光温室越冬西葫芦高产栽培技术

选择抗病、耐低温、高产优质的品种,经过处理的种子在日光温室内建造苗床育苗,幼苗出土前,苗床气温白天保持28～30℃,夜间保持16～20℃。起垄定植,当幼苗三叶一心,苗龄25～30d时即可定植。定植后管理：浇一次大水、吊蔓、落蔓、肥水管理、温度控制、蘸花保果等等一系列措施。生长期要进行全面病虫害防治,以达到高产效果。     

《青岛市新型职业农民培育管理办法》立法调研报告

为了促进和规范新型职业农民培育工作,提高农民科学文化素质和从业能力,加快培育新型职业农民和现代农业新型经营主体,青岛市农委将《青岛市新型职业农民培育管理办法》列入2013~2015年青岛市立法论证和调研计划。调研组于2013年3月至2016年3月,通过查阅文献资料、实地调研、问卷调查、召开座谈会等方式开展立法调研工作,认为《青岛市新型职业农民培育管理办法》政府规章的出台十分必要。     

封闭型植物工厂发展现状与展望

经过多年探索和发展,封闭型植物工厂无论在材料、装置和技术都取得了快速进步,生产产量成数倍增长。在介绍发展封闭型植物工厂必要性的基础上,着重分析了封闭型植物工厂的发展现状、设施分类、栽培形式选择、栽培技术选择,最后指出了存在的问题,并对未来的封闭型植物工厂外部设施及其栽培技术进行了展望。     

深松整地作业技术要点

根据康平县在实施深松整地作业中遇到的实际问题,介绍深松机具的选择原则、作业质量标准、作业时间确定方法,以及作业技术要点和注意事项,为提高全县深松整地作业质量提供参考。     

反向遗传技术在牛轮状病毒研究中的应用

本文综述了牛轮状病毒的生物学特性、反向遗传学系统的建立及其研究进展,并对反向遗传技术应用于牛轮状病毒进行展望。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.