祁连山森林土壤的水文生态效应

祁连山水源涵养林受立地水热条件的影响和自然、历史干扰,多世代演替共存,分布占祁连山3个垂直气候带,主要由湿性灌丛林、青海云杉林、祁连圆柏林、干性灌丛林4种类型组成,6个林型即湿性灌丛林、藓类青海云杉林、灌木青海云杉林、草类青海云杉林、祁连圆柏林、干性灌丛林。调查研究表明,各类森林枯落物(层)的现存量,组成成分、持水率、持水量等均不相同。森林土壤矿质层的主要物理性质因森林类型而不同,并均随着土壤的深度呈某种规律性的变异。森林土壤的持水量主要受土壤孔隙度的影响。在祁连山林区,森林土壤的持水量较非林地(牧坡草地)土壤为高,在各森林类型中青海云杉林和灌丛林的持水量,较祁连圆柏林为高。     

不同水分条件下皮叶两用杜仲林的生长效应

对12年生杜仲进行人工供水处理,研究土壤水分对皮叶两用杜仲林萌条生长规律的影响.结果表明:不同水平的供水对新枝生长均有促进作用,6~9月份,Ⅰ、Ⅱ、Ⅲ、Ⅳ级供水处理的新枝枝长生长量分别较对照增加30.2%、37.1%、77.9%、56.2%,新枝直径生长量分别较对照增加22.4%、53.7%、117.9%、83.6%;Ⅲ级供水最有利于萌条的生长,二次分枝数最多(43个/株),平均枝条长度最长(27.3 cm),单株叶片数和叶干质量分别为162个.株-1和16.5 g.株-1;杜仲供水多时,水分并不能被根部全部吸收,水分利用效率降低;5~9月份,秦岭南坡杜仲适宜的灌水量为1 050 m3.hm-2.     

Post-fire habitat restoration of sables during winter season in northern slope of the Great Xing＇an Mountains

Habitat loss and fragmentation have been associated with the decline of endangered species. In 1987, a catastrophic fire in the northern Great Hing＇an Mountains of China, where the main habitat of sables （Martes zibellina） is located, aggravated the loss and fragmentation of the forest landscape. Due to restricted distribution and low population density, sables were listed in the national first-grade protected species in China. The objective of this paper was to identify to what extent the habitat of sables had been restored 13 years after the fire. Based on the behavioral data, which came from field survey information by radio-tracking, GPS （Global Positioning System） and forest inventory data, suitability habitat maps were derived using the Ecological Niche Suitability Model （ENSM）. In addition, the habitat structure was analyzed with selected landscape indices. Although forest cover mostly had been restored by 2000, the results indicated that, compared to the pre-fire situation, the areas of suitable habitat had been reduced significantly, especially those of less suitable, marginally suitable and moderately suitable designation. Fragmentation was aggravated, and suitable patches were found to be further isolated with the exception of those in most suitable areas. The ratio of the patch perimeter to area in unsuitable, moderately suitable and suitable areas decreased, while the ratios within other suitability types increased. Moreover, the percentage of soft boundaries decreased slightly, which can influence the redistribution of sables. The results above indicated that the suitable habitat had deteriorated, and the restoration of the sables＇ habitat remained to be done.     

女儿寨小流域植被恢复与重建过程中的小气候效应研究

以女儿寨小流域为研究对象，着重研究了植被恢复重建过程中小流域系统的小气候变化特征。随着小流域内的植被恢复与重建，小流域内最高气温约降低了1～4℃，尤其以夏、秋季更为突出，约降低了3～4℃；最低气温提高了1～2℃，夏季较其它季节更为显著一些；森林不能降低小流域的日平均气温，但能略微增加秋冬季的平均气温0．1～0．5℃；平均地面温度降低了0．9～1．3℃，秋冬季的地面平均温度略微增加0．1～0．5℃；年降水量增加了77～115mm；年蒸发量降低105～175mm。小流域小气候环境发生了较大的变化，小气候质量在不断的提高。     

杉木初级种子园遗传效应的一个验证

从杉木林中采集七株优树的种子，并在种子园内采集相同优树无性系自由授粉种子，进行成对对造林比较试验，造林四年后，来自种子园的后代其生长量显著高于来自林分的后代，树高增长9．1％，胸径增加14．5，由此，对杉木初级种子园的遗传效应加以肯定并进行评论。     

不同立地条件营造香椿用材林效果及经济效益分析

以福安市1999年营造的香椿用材林林分为对象,进行造林效果和经济效益分析。结果表明:在不同的立地条件下,营造香椿用材林林分胸径、蓄积量差异显著,随着林分林龄的增大,香椿的胸径、蓄积量差异增大;Ⅰ、Ⅱ类地培育香椿用材林内部收益率在15.56%以上,能带来较好的经济效益,Ⅲ类地培育香椿用材林内部收益率在-5.31%,不能带来经济效益。培育香椿用材林应选择Ⅰ、Ⅱ类地造林,以选择Ⅰ类地最佳。     

膜分离法纯化浓缩的猪细小病毒灭活疫苗效果研究

为了研究纯化浓缩的猪细小病毒灭活疫苗的免疫效果,应用微滤/超滤管式陶瓷膜分离系统对猪细小病毒细胞收获液进行纯化浓缩,使浓缩液病毒平均含量由10~(4.7)TCID_(50)/mL提高到10~(6.5)TCID_(50)/mL;将纯化浓缩的病毒液经检验合格后,进行甲醛灭活,与注射用矿物白油佐剂制成油包水灭活疫苗;按照猪细小病毒灭活疫苗的质量标准,对制备的灭活疫苗进行检验与免疫效果试验。结果显示:纯化浓缩的猪细小病毒液杂蛋白去除率平均达到71.6%左右;制备的三组灭活疫苗检验均合格;免疫至63 d时,免疫猪体内抗猪细小病毒抗体(HI)水平分别为:纯化浓缩的灭活疫苗平均高达9.62 log_2稀释倍数,常规疫苗平均为8.78log_2稀释倍数,差异显著(P0.05)。试验表明:疫苗病毒细胞收获液进行膜纯化技术处理后,免疫效果显著优于未经纯化的常规灭活疫苗。     

蚕沙和稻秸不同比例组合对瘤胃微生物体外发酵的组合效应

本文采用瘤胃微生物体外发酵法研究了不同比例蚕沙(SE)和稻秸(RS)的组合效应。试验将SE:RS设计为100∶0(SE100组)、80∶20(SE80组)、60∶40(SE60组)、40∶60(SE40组)、20∶80(SE20组)、0∶100(SE0组)的比例,分别进行体外发酵批次培养24 h和体外发酵产气培养72 h,测定产气参数和发酵特性指标。结果表明:1)SE100组理论最大产气量显著高于其他各组(P<0.05),SE80组的24和72 h累积产气量显著高于其他组(P<0.05)。2)24 h时培养液总挥发性脂肪酸浓度为SE80组>SE100组>SE60组>SE40组>SE0组>SE20组,乙酸/丙酸为SE80组SE100组>SE60组>SE80组>SE40组>SE0组。4)随着蚕沙比例的升高,底物体外有机物消化率越高。5)SE80组多项指标组合效应指数为0.76,SE80组>SE40组>SE60组>SE20组。蚕沙和稻秸组合改善了体外瘤胃微生物发酵特性和产气参数,且蚕沙和稻秸的最佳比例为80∶20。     

Sodium bicarbonate protects uranium-induced acute nephrotoxicity through uranium-decorporation by urinary alkalinization in rats

To evaluate the effectiveness of sodium bicarbonate (SB) in removing uranium and protecting animals from uranium toxicity, we intramuscularly administered 1 mg/kg of uranyl nitrate to 8-wk-old male SD rats, and 20 min after administration of uranyl nitrate, the animals were given a single oral administration of SB at 0.1, 0.3 or 1 g/kg. The SB treatment at a dose of 0.3 g/kg or more raised the pH of the rats’ urine until 4 h after treatment, and it significantly reduced the uranium amounts in the kidneys at 1 day after treatment. In another experiment, rats were intramuscularly administered 1 mg/kg of uranyl nitrate, and 20 min later, the animals were treated with sodium bicarbonate (0.1 or 1 g/kg). The rats were autopsied at 1, 3 and 7 days after uranium treatment. High-dose SB resulted in a significant increase in urinary uranium excretion in the first 24 h and a reduction of uranium deposition in the kidneys and femurs, and it also significantly suppressed uranium-induced renal toxicity, as shown by both histopathology and clinical chemistry at 3 days after uranium treatment. Low-dose SB did not show such marked effects. Our findings demonstrated that the uranium decorporation effect of sodium bicarbonate was observed at the dosage showing urine alkalinization in rats and that decorporation effect of sodium bicarbonate might be beneficial if it is administered immediately after incorporation of soluble uranium.     

The pharmacological effects of the anesthetic alfaxalone after intramuscular administration to dogs

The pharmacological effects of the anesthetic alfaxalone were evaluated after intramuscular (IM) administration to 6 healthy beagle dogs. The dogs received three IM doses each of alfaxalone at increasing dose rates of 5 mg/kg (IM5), 7.5 mg/kg (IM7.5) and 10 mg/kg (IM10) every other day. Anesthetic effect was subjectively evaluated by using an ordinal scoring system to determine the degree of neuro-depression and the quality of anesthetic induction and recovery from anesthesia. Cardiorespiratory variables were measured using noninvasive methods. Alfaxalone administered IM produced dose-dependent neuro-depression and lateral recumbency (i.e., 36 ± 28 min, 87 ± 26 min and 115 ± 29 min after the IM5, IM7.5 and IM10 treatments, respectively). The endotracheal tube was tolerated in all dogs for 46 ± 20 and 58 ± 21 min after the IM7.5 and IM10 treatments, respectively. It was not possible to place endotracheal tubes in 5 of the 6 dogs after the IM5 treatment. Most cardiorespiratory variables remained within clinically acceptable ranges, but hypoxemia was observed by pulse oximetry for 5 to 10 min in 2 dogs receiving the IM10 treatment. Dose-dependent decreases in rectal temperature, respiratory rate and arterial blood pressure also occurred. The quality of recovery was considered satisfactory in all dogs receiving each treatment; all the dog exhibited transient muscular tremors and staggering gait. In conclusion, IM alfaxalone produced a dose-dependent anesthetic effect with relatively mild cardiorespiratory depression in dogs. However, hypoxemia may occur at higher IM doses of alfaxalone.