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251.
D. Lippi M.R. De Paolis E. Di Mattia S. Grego T. Pietrosanti I. Cacciari 《Biology and Fertility of Soils》2000,30(4):276-283
The effect of salinity on growth, starvation-survival and recovery from salt stress of a Rhizobium sp. strain isolated from nodules of Acacia tortilis from a soil of Senegal was studied. Growth parameters of C-limited continuous cultures, grown in the presence and the absence
of 342 mM NaCl, decreased in the saline medium and with increasing dilution rates. The survival capacity of starved cultures
depended on the previous growth conditions: culturability of cells grown with salt was inversely related to growth rate, while
culturability increased with increasing dilution rate for cultures grown without salt. Culturability of the cultures subjected
to the double stress of starvation and salinity was reduced and a high percentage of cells entered the viable but nonculturable
state. All the starved cultures were capable of regrowth when nutrients became available, thus showing that this strain can
withstand long periods of nutrient deprivation in soil while maintaining the capacity for an active metabolism and a potential
infectiousness toward an appropriate host.
Received: 14 December 1998 相似文献
252.
观赏植物试管苗玻璃化现象及防治研究进展 总被引:8,自引:0,他引:8
综述了离体培养中观察到有试管苗玻璃化现象的观赏植物种类,玻璃苗的形态解剖和生理生化特点,影响试管苗玻璃化的因素及克服试管苗玻璃化措施的研究进展。 相似文献
253.
254.
美味猕猴桃原生质体培养及植株再生技术研究 总被引:11,自引:1,他引:10
以美味猕猴桃雄株茎段愈伤组织为材料,分离原生质体,并培养在KM8P附加0.45mol/L葡萄糖和0.05mol/L蔗糖的培养基上,用低熔点琼脂糖包埋,6 ̄7d发生第一次细胞分裂,培养20d的分裂率为11.3%,在未添加新鲜培养液的情况下,原生质体再生的细胞可持续分裂至80d左右,并形成2 ̄3mm大小的愈伤组织,然后采用二步诱导分化法将原生质体来源的愈伤组织诱导分化出绿苗,再诱导生根,形成完整的小植 相似文献
255.
256.
Three cell lines of groundnut (Arachis hypogaea L), an important oilseed legume, were selected on glyphosate using in-vitro culture techniques. The cell lines isolated through single as well as stepwise selection procedures showed c 20-fold increase in glyphosate tolerance as compared to the unselected control cell line. Studies on the biochemical mechanism of glyphosate tolerance in these cell lines showed a significant increase in the total extractable activity of the target enzyme, 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19), which was further confirmed with immunological data. The over-expressed EPSP synthase activity was, however, subject to inhibition by glyphosate in vitro. Two other key regulated enzymes of the shikimic acid pathway, 3-deoxy-D -arabino heptulosonate 7-phosphate (DAHP) synthase (EC 4.1.2.15) and chorismate mutase (CM) (EC 5.4.99.5) did not show any change in specific activity in the selected cell lines. The enhanced activity of EPSP synthase in the tolerant cell lines was found to be stably inherited in the absence of selection pressure. © 1999 Society of Chemical Industry 相似文献
257.
Pamela J. Hatton Ian Cummins Lindsey J. Price David J. Cole Robert Edwards 《Pest management science》1998,53(3):209-216
Glutathione transferases (GSTs) catalysing the conjugation of 1-chloro-2,4-dinitrobenzene, the chloro-s-triazine herbicide atrazine, the chloroacetanilide herbicides metolachlor and alachlor and the diphenyl ether herbicide fluorodifen have been identified in suspension-cultured cells derived from the grass weed giant foxtail (Setaria faberi Herrm.). In contrast to suspension-cultured cells of maize, where atrazine-conjugating GSTs are lost during de-differentiation, the GSTs active toward this herbicide in S. faberi plants were also expressed in cultures, suggesting that these isoenzymes are subject to different regulation in the crop and weed. As a result, glutathione conjugation was the major route of atrazine metabolism in S. faberi cultures. Activities of these GSTs were maximal three days after sub-culturing when the cells were dividing most actively, when they were determined to be in the order CDNB>alachlor>metolachlor= fluorodifen>atrazine. This indicated that GSTs which are enhanced during cell division can metabolise herbicides. On the basis of activity per mg protein, GST activities in the cultures were between 20 and 60-fold higher than those determined in the foliage of S. faberi seedlings. The GSTs with activity towards CDNB were resolved into three peaks following anion-exchange chromatography at pH 7·8 using Q-Sepharose. Peak 1 GSTs were not retained, while peak 2 and peak 3 were sequentially resolved with an increasing concentration of salt. Peak 1 GSTs showed activity toward metolachlor and atrazine but showed little activity toward fluorodifen. Peak 2 and peak 3 GSTs were active toward atrazine and metolachlor, with peak 3 being particularly associated with activity toward fluorodifen. The GSTs in these peaks were then further purified using S-hexyl-glutathione-agarose affinity chromatography. In each case, the affinity-bound fraction of the GSTs consisted of 28 kDa and 26 kDa polypeptides, suggesting that the GST isoenzymes in S. faberi cultures are composed of related subunits. Our results demonstrate that the GST isoenzymes involved in herbicide metabolism in suspension cultures of a grass weed show a similar level of complexity to that determined in maize cell cultures. © 1998 SCI 相似文献
258.
A protocol was developed for direct shoot and plantlet regeneration from in vitro regenerated leaf explants of male Pistacia vera L. cv. ‘Atl?’. Leaves excised from axenic shoot cultures of pistachio were used to induce organogenesis on a Murashige and Skoog (MS) medium with Gamborg vitamins supplemented with combinations of different concentrations of BAP and IAA. The highest adventitious shoot regeneration in 35% of the explants, with the number of shoots ranging from 2 to 3 per explant, occurred in the explants cultured during the establishment phase in the medium with 1 mg l−1 IAA and 2 mg l−1 BAP. For shoot multiplication, the highest number of new microshoot/explants (5.76) was obtained in a culture medium supplemented with 1 mg l−1 BAP, but it was not significantly different from the number obtained at 2 mg l−1 BAP. A high rooting frequency (84%) for microshoots was recorded on a medium supplemented with 2 mg l−1 IBA. In vitro rooted plantlets were transferred to pots filled with a mixture of soil, sand and peat (1:1:1). They were weaned in a growth room and finally moved to a greenhouse. This protocol could be utilized for in vitro clonal propagation of this economically important plant. 相似文献
259.
260.
硼钙营养对柠檬幼苗光合生理及根系活力的影响 总被引:1,自引:0,他引:1
采用二因素五水平回归最优设计、砂培,两种pH环境(pH15.6~5.8,pH27.6~7.8),研究了B,Ca不同浓度对柠檬幼苗生长、叶片叶绿素含量、光合强度及根系活力的影响,建立了相应的数学模型。结果表明,B,Ca缺乏或过量降低叶绿素含量和光合强度。叶绿素含量最大时的叶Ca/B分别为pH1下491,pH2下293;光合强度最大时的叶Ca/B分别为pH1下525,pH2下285。B,Ca浓度适量下降提高了根系活力,而B,Ca浓度过量抑制了其活力,碱性环境的根系活力明显低于酸性环境。 相似文献