南方鲶SME－Ⅰ细胞系的建立及其生物学特性的研究

用半消化法和组织块法接种南方鲶晚期胚，经４０多代连续培养，建立了ＳＭＥ－Ⅰ鲶鱼细胞系。该细胞系为贴附性上皮样细胞。细胞内微丝束丰富，平行排列伸到伪足，但质膜下不很密集。细胞最适在含１５％～２０％小平血清的１６４０培养基中生长，适温２８～２９℃，ｐＨ６．５～７．２，群体倍增时间为５８．６ｈ，已增殖到１０￣８个的水平。细胞染色体数为正常二倍体２ｎ＝５８，但有不少异倍化细胞。该细胞系已可用于鱼类生物工程研究和进入细胞资源库保存。     

A Remote Sensing Characterization of the Effects of Anastrepha striata ＆ Anastrepha ludens on Guava Cultures in Mexico

Detection of the presence of Anastrepha striata and Anastrepha ludens in guava cultures is presently done in Mexico by actually catching the flies in strategically located traps. The objective of this study is to show that it is possible to locate areas with the presence or absence of flies by remote sensing means, thereby simplifying the traditional way of detection of these plagues. In the field, groups of traps were selected where flies had been captured, as well as areas in which there was no capture in the traps. The radiometric signatures of whole trees and leaves were obtained, showing measurable differences between those specimens affected by the plague and those unaffected. Next we selected a SPOT5 image of 2007 corresponding to the study area in Calvillo, State of Aguascalientes, in Mexico, one of the major areas of guava cultures in the country. A supervised classification of the image allowed for the location of guava cultures in it. The guava culture areas obtained from this classification were validated comparing them to available maps of the cultured areas. Spectral signatures for the classes were derived from the image data. The separability of pairs of classes was also evaluated in order to maximize it. The IR/R （infrared to red ratio） ratio of the image bands was evaluated in 80 × 80 pixels around the locations of five traps where flies had been captured, and around five locations where the traps had not captured flies. Only the pixels where guava cultures were present were included in the analysis, other types of vegetation and soil coverage were rejected. We found that the index distributions with flies captured and those without flies captured cluster in two well-separated groups. We note that plotting the whole distribution of pixels around a trap yields a diagnostic view of the area, and individual index values do not provide such a view, since values with the flies＇ presence and without these overlap to some extent. Further analyses of other trap locations confirmed this separation     

制造执行系统MES在水泥行业中的应用

对于任何社会,礼都是不可或缺的。《南华真经》一方面反对世俗礼制,认为俗礼的出现标志着人际关系的疏远,是“相伪”的表现,会造成财富的浪费,甚至堕落为人们盗取名利的工具,促使人性变坏;另一方面,又提倡能够表达真情、带有理想化的“至礼”。 然而当《南华真经》面对现实社会时,又不得不在不同的层面,以各种不同的方式兼顾了“俗礼”和“至礼”。     

英语跨文化交际教学的思考

英语教学的根本目的就是为了实现跨文化交际，教师应意识到跨文化教学是英语教学的一个重要环节，在教授语言的同时同步传授文化知识。本文论述了跨文化教学的内容和必要性，阐述了在英语教学中如何进行跨文化教学。     

传染性支气管炎病毒M41和Ma5的气管环培养特性研究

本试验以鸡胚气管环纤毛运动作为指示系统，研究传染性支气管炎病毒IBV-M41和IBV Ma5的气管环培养特性。用100 TOC-ID₅₀病毒量的两毒株分别感染鸡胚气管环培养物，观察感染后不同时间气管纤毛的存活率。结果表明，IBV-M41毒株致死气管环上皮细胞的速度要比毒株IBV-Ma5快，说明2个毒株的培养特性与毒力一致。     

论我国古代思想道德教化方法的层次性及其德育功能

我国古代思想道德教化方法是一个分别从家庭、学校、社会等层面建构起来的结构严密的方法体系，解读这些在社会不同层面设计的有效教化方法及其发挥的德育功能，对于提高当前我国思想道德教育的效果，仍具有积极的启迪作用。     

“中国诠释学”的发展现状及其存在的问题

数年来,有关“中国诠释学”的研究开展得如火如荼,其中尤以傅伟勋教授、成中英教授、黄俊杰教授和汤一介教授的研究最具代表性。前人的摸索为我们积累了可贵的经验,但是,已有的几种“中国诠释学”体系中也暴露出一些应予关注的问题,如：“中国诠释学”的构建不能只在方法论层面上下功夫,还须进行本体论层面的梳理;对西方诠释学的借鉴应当有所选择,无区别的移植只会损害“中国诠释学”应有的特质。     

Diagnosis of mixed infections with myxovirus influenzae A equi 2 and herpes virus equi 1 among Danish stud horses

Examination of nasopharyngeal secretion and organ material from clinical cases of respiratory diseases in horses, using inoculation of embryonated hen eggs and rabbit and horse kidney cell cultures, resulted in the isolation of influenza virus and herpes virus. In 2 cases, both viruses were present in the same specimen.On the basis of the physio-chemical, cytological and serological criteria, the viruses were found to be identical with influenza virus type A equi 2 and herpes virus equi type 1.The methods for serological diagnosis and characterization of the influenza and herpes viruses are discussed. Detailed serological examinations of blood specimens from the clinical cases confirmed the aetiological significance of the isolates and the presence of mixed infections.Further characterization of a strain of influenza virus was carried out by inoculation experiments on horses. These included reproduction of clinical influenza, reisolation of the strain in casu and a study of the antibody formation.     

Metabolism of the herbicide glufosinate‐ammonium in plant cell cultures of transgenic (rhizomania‐resistant) and non‐transgenic sugarbeet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium)

The metabolism of the herbicide glufosinate‐ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar‐gene) and non‐transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). ¹⁴C‐labelled chemicals were the (racemic) glufosinate, L ‐glufosinate, and D ‐glufosinate, as well as the metabolites N‐acetyl L ‐glufosinate and 3‐(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non‐extractable residues ranged from 0.1% to 1.2% of applied ¹⁴C. Amounts of soluble metabolites resulting from glufosinate or L ‐glufosinate were between 0.0% and 26.7% of absorbed ¹⁴C in non‐transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D ‐Glufosinate, MPP and N‐acetyl L ‐glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N‐acetyl L ‐glufosinate, besides traces of MPP and 4‐(hydroxymethylphosphinyl)butanoic acid (MPB). In non‐transgenic sugarbeet, glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea. © 2001 Society of Chemical Industry     

日本竹文化现象及其内涵

从中日竹文化关系、日本竹文化现状以及日本竹文化现象等方面进行了细致考察和梳理,进而从竹之语源的角度揭示出日本竹文化的内涵特征。日本竹文化在形成和发展的过程中,吸收了中国竹文化的内容,形成了独特的竹文化。与中国竹文化相比,日本竹文化应用领域更加广泛。日本竹文化既体现了日本民族的生活习惯、心理素质和审美情趣,又展示了日本民族的内心世界和精神追求。竹子不仅在物质上与人们的生活息息相关,在人们的精神生活中也发挥着特殊的作用。