马占相思扦插快繁育苗技术研究

通过双因素试验设计研究了3种不同木质化程度插穗(A)、4种不同浓度生根促进剂ABT-1#(B)和不同扦插基质(C)对马占相思扦插生根率、生根数及根长的影响差异,结果表明:未木质化的嫩枝(A1)+生根促进剂ABT-1#400 mg.kg-1(B2)生根率高、生根数多及生根长,生根率最高达83.3%;不同基质扦插中以未木质化的嫩枝(A1)+生根促进剂ABT-1#400 mg.kg-1(B2)+80%红心土和20%珍珠岩混合基质最佳。     

沙地彰武松容器育苗基质配比研究

在彰武松容器育苗过程中进行了育苗基质不同配比和不同施肥对比试验,结果表明,最适合彰武松容器苗高生长的基质配比草炭∶沙壤土∶三元素复合肥为1∶1∶0.05,其平均苗高为28.4 cm,方差分析表明各处理间差异极显著。     

高效液相色谱法测定复方非泼罗尼滴剂中抗氧剂的含量

建立高效液相色谱法(HPLC)测定复方非泼罗尼滴剂中抗氧剂叔丁基对羟基茴香醚(BHA)与二丁基羟基甲苯(BHT)含量。采用高效液相色谱法,色谱柱为Agilent Zorbax SB-C18(250×4.6 mm, 5μm),流动相A为乙腈-甲醇-水(47∶21∶32),混合前在水中加入1%冰醋酸,流动相B为乙腈,线性梯度洗脱;检测波长为285 nm;流速为0.9 mL/min;柱温为30℃;进样量为20μL;采用外标法计算BHA、BHT含量。在建立的色谱条件下,空白溶液、阴性样品对BHA、BHT的测定均无干扰;BHA、BHT的检测限分别为0.08μg/mL和0.19μg/mL,定量限分别为0.31μg/mL和0.77μg/mL,分别在5.13～15.39μg/mL与2.56～7.66μg/mL范围内线性关系良好;平均回收率分别为98.5%和99.9%(n=9)。所建立的方法简单易行、专属性强、准确度高、灵敏度好,可用于复方非泼罗尼滴剂中抗氧剂的含量测定。     

酵母基因表达调控关系的构建及其统计特性分析

[目的]从复杂网络的角度研究酵母细胞周期调控网络的统计特性。[方法]首先对酵母的细胞周期基因表达数据采用皮尔逊相关性度量,计算基因间的关联矩阵,即建立简单的基因调控网络;同时为了能够得到在宏观角度对大规模的基因调控有一个全貌的了解,进一步分析所建立调控网络的统计特性,通过计算所建立网络的统计特性：即计算网络的聚类系数、平均路径长度和网络的度分布。[结论]发现所建立的网络具有无标度和小世界特性,即所建立的酵母基因调控网络属于复杂网络,这为进一步深入研究基因调控网络的统计学和动力学特性打下了基础。     

基于Matlab的AHP分析程序设计

层次分析法是目前最为广泛应用的决策方法,但涉及大量的矩阵运算,影响了该方法的推广。Matlab软件精于矩阵运算,与C语言结构类似,移植性强,本研究基于Matlab设计出了便捷高效的AHP计算机程序。     

复方黄柏颗粒剂在小鼠体内的药代动力学

为探讨复方黄柏颗粒剂有效成分-盐酸小檗碱在小鼠体内的药动学特征,按25 g/kg(相当于盐酸小檗碱10.95 mg/kg)的剂量经口给予小鼠复方黄柏颗粒剂,用高效液相色谱法检测用药后不同时间血浆中盐酸小檗碱的质量浓度,研究在小白鼠体内的药物动力学。结果表明口服复方黄柏颗粒的有效成分-盐酸小檗碱在小鼠体内的药时数据符合开放性二室模型,动力学方程为C=17.8e-0.859 81t 14.51e-0.636 1t。主要药代动力学参数:t1/2α为(0.81±0.07)h,t1/2β为(5.25±2.96)h,t1/2Ka为(0.76±0.05)h,AUC0→∞为(3.389 3±0.437 4)mg.L-1.h,CL/F为(3.281 8±0.446 3)L/h,Vd/F为(13.65±22.38)L/kg,Tpeak为(1.617 19±0.056 50)h,Cmax为(1.149 98±0.056 04)μg/mL。     

苹果轮纹病菌复配药剂室内筛选试验

采用生长速率法进行多菌灵、甲基托布津、扑海因、三唑酮、代森锰锌、福美双6种药剂对轮纹病菌的毒力测定.结果表明:多菌灵、甲基托布津和扑海因对轮纹病菌具有较好的抑制作用;采用生长速率法和孢子萌发抑制试验,进行多菌灵∶扑海因=1∶1、多菌灵∶扑海因=2∶1、甲基托布津∶扑海因=1∶1、甲基托布津∶扑海因=2∶1这4种混配药剂对轮纹病菌毒力测定,结果表明:除了多菌灵∶扑海因=2∶1的处理外,其余3个处理共毒系数均大于100,说明混配后具有明显的加性效应,其配比可作为药剂加工的混配比例.     

The spore coat of the bean anthracnose fungus Colletotrichum lindemuthianum is required for adhesion, appressorium development and pathogenicity

The spores (conidia) of the bean anthracnose fungal pathogen, Colletotrichum lindemuthianum, adhere to the aerial parts of plants to initiate the infection process. In previous studies we have shown that the Colletotrichum spores are surrounded by a fibrillar spore coat, comprising several major glycoproteins. Previous evidence showed that a monoclonal antibody (UB20) that recognised these glycoproteins was able to inhibit adhesion of spores to a hydrophobic surface. In this paper we have further studied the role of the spore coat in adhesion, germination and fungal development by studying the effects of UB20 and protease treatment of spores. The latter treatment has previously been shown to remove the spore coat. Spores germinate on glass, polystyrene and water agar, however, appressoria only develop on glass or polystyrene, showing a requirement for a hard surface. Removal of the spore coat with protease inhibits adhesion at 30 min, before the secretion of ECM glycoproteins. Protease treatment also inhibits the development of appressoria and reduces pathogenicity on leaves. Incubation of spores with the MAb UB20 inhibits adhesion at 30 min, but does not affect appressorium formation or pathogenicity. The results suggest that an intact spore coat has two functions; it is required for adhesion to a hydrophobic surface and for the detection of a hard surface necessary for appressorium formation. We suggest that contact with a hard surface, rather than adhesion, is the key event leading to appressorium formation.     

Influence of Sucrose, Mucin and Xanthan Gum on Spore Germination of Ten Different Fungi

Germination of conidia, uredospores and basidiospores of ten diverse fungi were compared in sterile distilled water, 0.5% sucrose, 0.5% mucin and 0.5% xanthan gum. All three compounds generally increased spore germination on cellophane membranes compared to water, but mucin was the most effective. Mucin stimulated significantly more germination than sucrose for 7 of the 10 fungi, and only Aspergillus niger and Colletotrichum gloeosporioides showed greater spore germination in sucrose than in mucin. Sucrose had no effect on uredospore or basidiospore germination, but mucin stimulated germination of these spores. Mucin was more effective than xanthan gum in increasing spore germination for 9 of the 10 fungi. Animal mucin is chemically similar to the natural spore matrix of some fungi and may provide some of the benefits of the spore matrix without the drawback of possible germination inhibitors. Animal mucin may be a useful substitute for the spore matrix in inoculations of a variety of fungi.     

基于RS和GIS的郑州市土地利用动态变化及预测

以郑州市为研究对象,选用1995年、2000年、2007年3期的TM图像,运用RS和GIS技术分析了郑州市1995-2007年间土地利用动态变化的空间过程,并运用马尔柯夫模型对土地利用动态变化及演变趋势进行了预测分析.结果表明:在12 a间,各土地类型的单一土地利用动态度都在增加,综合土地利用动态度也呈上升趋势.预测结果表明,2007年以后的14 a内(2007-2021年)研究区内耕地将进一步减少,但减少的幅度将逐渐减小;林地总面积也将减少,但草地的面积将变化不大;城镇用地将进一步继续增加,但增加的速度将放缓;水域面积将继续减少.