不同剂量环磷酰胺对昆明小鼠肝肾组织的损伤作用研究

为研究不同剂量的环磷酰胺对小鼠肝肾组织及功能的损害程度,试验选取40只5周龄健康雌性昆明小鼠,随机分为5组,除对照组外,其余4组小鼠连续3 d分别腹腔注射40、80、120及160 mg/（kg·BW）环磷酰胺溶液,注射后第7天采集肝脏和肾脏组织样品及血清,制备石蜡组织切片及透射电镜切片,观察肝脏和肾脏的组织学变化,检测血清中甘胆酸（CG）、胆碱酯酶（ChE）、血清前白蛋白（PA）、总胆汁酸（TBA）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、尿素氮（BUN）、血清肌酐（SCr）、尿酸（UA）含量/活性。结果显示,环磷酰胺可引起小鼠肝肾组织病理损害,呈剂量依赖性,肝脏比肾脏更早出现损伤。石蜡切片及透射电镜切片观察结果显示,环磷酰胺对肝脏的毒性作用主要表现在肝索紊乱、肝血窦扩张、微胆管淤胆、门管区及中央静脉周围炎性细胞浸润及纤维增生、组织间出血、肝细胞出现脂肪变性及气球样变性,凋亡及坏死细胞增多。肾脏组织损伤主要表现在出血及纤维增生,肾小管上皮细胞胞浆空泡样变、线粒体损伤、核固缩,上皮细胞基底膜增厚。环磷酰胺对膀胱的损害不严重。与对照组相比,40~160 mg/（kg·BW）剂量组小鼠血清中ChE活性及PA水平,120~160 mg/（kg·BW）剂量组AST活性,80~160 mg/（kg·BW）剂量组BUN水平及80 mg/（kg·BW）剂量组UA水平均显著升高（P<0.05）;其余生化指标与对照组差异不显著（P>0.05）。以上结果表明,环磷酰胺注射剂量在80~120 mg/（kg·BW）时可引起昆明小鼠肝脏和肾脏组织及细胞明显的病理损伤。该研究结果可为选择合适的环磷酰胺注射剂量用以制备动物肝脏免疫抑制模型提供试验依据。     

The effects of maxillary nerve block,ethmoidal nerve block and their combination on cardiopulmonary responses to nasal stimulation in anesthetized Beagle dogs

ObjectiveTo describe an approach for ethmoidal nerve block (E_BLOCK) and to compare the effects of a maxillary nerve block (M_BLOCK), E_BLOCK and their combination (M-E_BLOCK) on heart rate (HR), systolic (SAP), mean (MAP), diastolic (DAP) arterial pressures and respiratory rate (f_R) during nasal stimulation in dogs.Study designProspective, blinded, randomized, crossover placebo-controlled study.AnimalsBeagle dogs (five cadavers, nine live dogs), with a median (interquartile range) weight of 10.5 (10.3–11.0) kg.MethodsThe accuracy of iohexol injections (each 1 mL) at the maxillary and ethmoidal foramina in cadavers was evaluated using computed tomography. Then, anesthetized dogs were administered four bilateral treatments separated by 1 week, saline or 2% lidocaine 1 mL per injection: injections of saline at the maxillary and ethmoidal foramina (Control), injections of lidocaine at the maxillary foramina and saline at the ethmoidal foramina (M_BLOCK), injections of saline at the maxillary foramina and lidocaine at the ethmoidal foramina (E_BLOCK) and injections of lidocaine at all foramina (M-E_BLOCK). The ventral nasal meatus was bilaterally stimulated using cotton swabs, and HR, SAP, MAP, DAP and f_R were continuously recorded. Values for each variable were compared before and after stimulation using Wilcoxon signed-rank test. Changes in variables among treatments were analyzed using Mann–Whitney U and Kruskal–Wallis tests (p ≤ 0.05).ResultsComputed tomography revealed iohexol distribution around the openings of the target foramina in all cadavers. In living dogs, HR, SAP, MAP, DAP and f_R significantly increased after stimulation within each treatment (p < 0.03). Physiologic responses were significantly attenuated, but not absent, in the M-E_BLOCK [HR (p = 0.019), SAP, MAP, DAP and f_R (all p ≤ 0.001)] compared with those in the Control.Conclusions and clinical relevanceConcurrent injections of lidocaine at the maxillary and ethmoidal foramina attenuated HR, arterial pressure and f_R responses to nasal stimulation in Beagle dogs.     

Corporate Dividend Policy Under the Change of Earnings in China

Using non-parameter test and multinomial logit regression, this paper studies the dividend policy and behavior empirically. The results show that the change of the earnings of firms affects their dividend policy. When the EPS of the firms increase, the probability of choosing not to pay cash dividend is maximal, yet when the EPS of the firms decrease, many firms choose not to pay cash dividend or choose to increase DPS, so that the information that the relevant firms have great expectation is transmitted to the market. Furthermore, this paper also finds that the successiveness of the dividend policy of the listed firms in China is not good.     

THE CHANGE LAW OF THE COAL''S STRUCTURE PARAMETER WITH INCREASING THE TEMPERATURE

In this paper,we have studied the change law of the structural parameter of coal in Nan Tong Coal Mine with the increasing the temperature. At the same time, we explore the cause of the changes with the electron microscope and of infrared spectrum.     

Agricultural practice changes with cultivating genetically modified herbicide-tolerant oilseed rape

To facilitate weed suppression, oilseed rape or canola (Brassica napus) has been genetically modified (GM) over the last two decades to incorporate herbicide tolerance (HT). The introduction of oilseed rape in commercial agriculture raises concerns about potential adverse agronomic and environmental effects linked to both the genetic modification and altered agricultural practice. How will this new crop modify the existing cropping practice? What are the potential agro-environmental implications of its introduction? This paper analyses and schematises recorded and conceivable agricultural practice changes with GMHT oilseed rape introduction and its likely agro-environmental effects. It develops a conceptual systematics of influencing factors, subsequent practice changes and likely agro-environmental effects. The results can be used to design the mandatory monitoring of adverse GM crop effects.     

新巴尔虎右旗植被覆盖变化研究

利用ArcGIS和ENVI的栅格空间分析工具,采用叠置分析、线性趋势分析法、均值法等分析方法,选用2001～2016年每年5～9月MODIS NDVI数据和该研究区域行政区划矢量数据来研究新巴尔虎右旗16年间的植被覆盖变化,为该地区植被趋势研究和生态建设提供一定的科学依据.结果表明:2001～2016年间新巴尔虎右旗生...     

55年来黄土高原边缘地区耕地动态变化的多尺度分析

本文对地处干旱、半干旱黄土高原生态脆弱地带的大同市耕地现状和1949—2003年间耕地总量变化进行分析,同时采用Morlet小波变换方法,对大同市耕地面积变化进行了多时间尺度和相应的周期变化特征分析,得出55年来大同市耕地面积变化具有2—3年和6年左右的年际尺度周期变化,并预测未来大同市耕地面积变化趋势;运用主成分分析方法分析得出耕地动态变化的驱动机制为人口因素、农业现代化水平、经济发展水平三个方面,所得结论有利于在科学的指导下采取措施稳定耕地面积,为干旱半干旱地区耕地的可持续利用提供科学依据,对相关省市农业可持续发展具有重要意义。     

峰丛洼地石漠化区景观格局的形态学特征及空间演变规律

[目的] 探究峰丛洼地石漠化区景观格局的形态学空间分布特征,为揭示峰丛洼地石漠化区景观格局动态变化和生态保护的可持续发展提供理论参考和指导。[方法] 利用形态学空间分析(MSPA)方法对岩溶石漠化景观识别、处理、分类得到核心、环线等互不重叠的7类景观类型,应用景观动态度、景观格局指数、景观转移矩阵和热点分析探究峰丛洼地石漠化区景观格局的形态学时空演变特征。 [结果] ①2000年形态学景观类型分布最广(190.60 km²),2022年分布最少(147.32 km²),在形态学景观类型中核心是研究区内主要的景观类型,2000年面积最大为121.62 km²,2022年面积最少为76.05 km²,主要分布在研究区西北部和南部区域;孤岛在形态学景观类型中面积最小,其面积1990年最少为1.12 km²,2022年最多为3.07 km²,孤岛和分支等景观类型分散分布在各核心之间。 ②研究区形态学景观空间分布趋于分散,且形态学景观多样性、复杂程度和景观破碎化程度增加;研究时段内,形态学景观类型单一动态度分别为0.10,-0.18和-2.13,说明形态学景观面积呈现收缩趋势并且形态学景观类型1990—2000年发育最为剧烈,2000—2010年发育最为平缓。 ③形态学景观类型转移方向主要是核心景观类型转移为边缘和孔隙景观类型,总体来看形态学景观主要转移方向是形态学景观转移为背景,并且背景的转入量大于转出量。高—高聚集区域呈现出向磨合村、老街村和安乐村扩张的趋势,低—低聚集区域呈现出向三光村和老街村扩张的趋势。 [结论] 研究区形态学景观面积处于快速减少阶段且形态学景观类型趋于复杂,核心是主要的形态学景观类型,形态学景观的演变特征主要由核心的变化导致。     

2000-2018年皖南山区土壤侵蚀时空变化

[目的]精确评估皖南山区土壤侵蚀量并分析其时空变化特征,为区域水土流失综合治理提供科学依据.[方法]在GIS技术的支持下,利用研究区日降雨数据,30 m分辨率DEM,土壤数据和土地利用等数据,采用中国土壤流失方程(CSLE)估算皖南山区土壤侵蚀模数,并分析2000,2010和2018年近20 a研究区土壤侵蚀时空变化特...     

1985—2018年内蒙古自治区耕地集约利用时空变化及其驱动因素

[目的] 探索内蒙古自治区耕地集约利用时空变化规律,为改善该区耕地集约利用方式,实现耕地资源可持续利用提供科学依据。[方法] 运用主成分分析方法对内蒙古自治区全区及其12个盟市的耕地集约利用水平进行综合评价,并揭示其时空变化特征及驱动因素。[结果] ①从全区层面分析,1985—2018年期间,内蒙古自治区耕地集约度总体呈增长态势,耕地集约度由63.30增长至88.12,增幅高达39.21%,耕地集约利用总体呈波浪式增长。②从空间层面分析,通过自然分界法将内蒙古自治区各盟市耕地集约利用水平划分为5个等级,其中巴彦淖尔市和呼伦贝尔盟为高度集约型(Ⅰ级),锡林郭勒盟、兴安盟、阿拉善盟、鄂尔多斯市为较集约型(Ⅱ级),呼和浩特市为一般集约型(Ⅲ级),赤峰市、通辽市为较粗放型(Ⅳ级),乌兰察布市、包头市、乌海市为粗放型(Ⅴ级)。③从驱动因素分析,人均GDP、农民人均可支配收入、财政用于农业支出以及第一产业固定资产投资额对内蒙古自治区耕地集约利用具有显著正向驱动作用,而第一产业生产总值、城镇化率以及治理水土面积对内蒙古自治区耕地集约利用具有显著负向驱动作用。[结论] 内蒙古自治区耕地集约度总体呈增长态势,未来应规范耕地流转,加强耕地用途管控,协调产量与生态的关系,进一步提高耕地集约利用程度。