室温下鸡蛋涂膜保鲜技术研究

通过对壳聚糖溶液、壳聚糖和氢氧化钙复合溶液、液体石蜡3种保鲜剂在常温下对鸡蛋的保鲜效果进行对比,结果表明：经过35d的储藏时间,发现氢氧化钙与2%壳聚糖溶液复合保鲜剂和液体石蜡保鲜剂的保鲜效果相当,好蛋率、失重率等指标均优于其他处理组;保鲜剂成膜的致密性和阻气性是影响鸡蛋储藏期的关键,而非传统观念上的抑菌性。     

缺钙时镧、铈对黄瓜白菜的生长及根系碱性磷酸酶活性的影响

在缺钙营养液中加入La~(3+) 、Ce~(3+),对黄瓜、白菜的生长产生有利影响,缺钙症状减轻,鲜重、株高、叶绿素、蛋白质含量均比缺钙培养有所提高.La~(3+)、Ce~(3+)可抑制根中碱性磷酸酶活性的升高.缺钙处理数天后,补加La~(3+)、Ce~(3+)也可减缓碱性磷酸酶活性的升高.这些生理作用与Ca~(2+)的作用相类似.     

盐旱交互胁迫对多花黑麦草(Lolium multilorum)种子萌发及胚生长的影响

为了揭示多花黑麦草(Lolium multilorum)对岩溶地区钙盐、干旱环境的适应机制,本研究采用不同浓度(0,5%,10%,15%,20%)聚乙二醇(PEG-6000)的干旱胁迫和不同浓度(0,5 mmol/L,10 mmol/L,25 mmol/L,50 mmol/L,100 mmol/L)CaCl2的钙盐胁迫,探讨干旱、钙盐及盐旱交互胁迫对多花黑麦草种子萌发及胚生长的影响。结果表明:随单一胁迫及交互胁迫浓度的增加,发芽势、发芽率、发芽指数呈先增后减的趋势,活力指数则不断下降。PEG浓度为10%时对多花黑麦草发芽率和发芽指数有促进作用;CaCl2浓度为5 mmol/L时对多花黑麦草的发芽势、发芽率和发芽指数有促进作用;5%PEG+10 mmol/L CaCl2交互胁迫下的发芽势、发芽率、发芽指数均较对照有所提高(p<0.05)。干旱胁迫下的胚芽长随胁迫浓度的增加呈先增后减的趋势,交互胁迫下胚根长及胚芽长均随胁迫浓度的增加而降低,15%PEG+100 mmol/L CaCl2、20%PEG+(5~100 mmol/L)CaCl2交互胁迫下种子不能萌发。本研究发现,岩溶区域低浓度的单一盐、旱胁迫和交互胁迫均对多花黑麦草种子的萌发有促进作用,干旱程度是影响该区域种子萌发和胚生长的限制性因子。本研究对多花黑麦草在岩溶地区的推广、种植有重要的指导意义。     

烤烟叶片成熟过程中钙镁铁含量的变化

田间条件下对烤烟下、中、上3个部位叶片成熟过程中钙、镁、铁元素含量的变化进行了分析。结果表明：随着烟叶部位的上升,叶片中钙、铁含量均表现为随之降低的变化规律,镁含量呈现出先降低又增加的变化;随着移栽后天数的增加,新鲜烟叶中各部位叶片钙、镁、铁含量无明显的变化规律性。3个烟叶部位之间钙、镁含量差异极显著,铁含量差异不显著;不同移栽后天数之间钙、镁、铁含量差异均不显著。移栽后天数与烟叶钙、镁、铁含量之间的相关性不显著;烟叶中钙与镁、钙与铁、镁与铁含量之间均呈极显著正相关。     

Ca2+对低温下水稻幼苗膜的保护作用

缺Ca2+的水稻幼苗在低温胁迫下(4℃36h)，细胞膜功能及超微结构破坏严重。在培养液中加入适当浓度的Ca2+(0.5～1.0mmol/L)可降低冷胁迫下稻苗的电解质渗漏率和MDA含量，提高SOD、CAT、POD活性，保护叶绿体和线粒体超微结构免遭破坏。CaM特异性抑制剂CPZ能部分抑制Ca2+降低冷害稻苗的电解质渗漏率及MDA含量和提高SOD、POD活性     

钙在黄皮种子萌发中的作用

本大以湿藏820天的典型顽拗性种子黄皮种子为材料，研究了钙对种子萌发的影响。在5～40mmol／LCa2 水平，低浓度Ca2 能提高黄皮种子的发芽率、活力指数和淀粉酶活性，随着浓度升高作用减弱，最适浓度为20mmol／L。EDTA和GA分别能降低和提高黄皮种子活力及淀酶活性。     

Early life permethrin exposure leads to hypervitaminosis D,nitric oxide and catecholamines impairment

The aim of this study is to gain more knowledge on the impact of early life pesticide exposure on premature aging.     

不同钙质肥料及钙肥不同用量对花生生长和产量的影响

在大田条件下,研究了不同钙质肥料及钙肥不同用量对花生生长和产量的影响。结果表明,施用等钙量不同钙质肥料(石膏粉、石灰粉、轻质碳酸钙)均能提高花生产量,但不同钙质肥料之间的差异不大。不同浓度梯度的氰氨化钙肥对花生产量影响显著。该新型氰氨化钙肥安全有效用量为75～225 kg/hm2,在150 kg/hm2用量时可获得最优农艺性状指数和最高经济效益。     

Effects of adipose tissue-derived mesenchymal stem cells on calcium channels of pulmonary artery in rats with pulmonary arterial hypertension induced by monocrotaline

AIM: To investigate the effects of adipose tissue-derived mesenchymal stem cells (ADMSCs) on calcium channels of pulmonary artery in monocrotaline (MCT)-induced pulmonary hypertensive rats.METHODS: ADMSCs were isolated from adipose tissue by collagenase digestion. Twenty-four Sprague-Dawley rats were randomly divided into 3 groups: normal control (Ctr) group, pulmonary arterial hypertension (PAH) group and ADMSCs transplantation group. Mean pulmonary arterial pressure (MPAP) was measured by catheterization, and right ventricular hypertrophy index (RVHI) was calculated. The expression of voltage-gated calcium channel α1c subunit (CaVα1c), sarcoplasmic/endoplasmic reticulum calcium ATPase 2a (SERCA-2a), inositol 1,4,5-triphosphate receptor 1(IP3R-1), transient receptor potential channel 1 (TRPC1) and TRPC6 at mRNA and protein levels in the pulmonary trunks was determined by RT-PCR and Western blotting, respectively.RESULTS: MPAP and RVHI were higher in PAH group than those in Ctr group, while those in ADMSCs group were significantly decreased as compared with PAH group. The expression of CaVα1c, TRPC1 and TRPC6 at mRNA and protein levels was obviously increased in PAH group as compared with Ctr group, while that in ADMSCs group was significantly decreased as compared with PAH group. Compared with Ctr group, the expression of SERCA-2a and IP3R-1 at mRNA and protein levels was obviously decreased in PAH group, while that in ADMSCs group was significantly increased as compared with PAH group.CONCLUSION: MPAP and RVHI are attenuated by ADMSCs in MCT-induced pulmonary hypertensive rats. The reduction of pulmonary arterial pressure by ADMSCs transplantation in MCT-induced pulmonary hypertensive rats may be related to the changes of calcium channels.