水泡性口炎病毒感染BHK-21细胞的超微结构改变

应用起薄切片技术对水泡性口炎病毒（VSV）在BHK-21细胞上的形态发生和早期发育进行了观察。结果表明，VSV能导致BHK-21细胞圆缩．胞浆严重空泡化，线粒体嵴断裂及空泡化；病毒在细胞浆内复制．增殖．在细胞膜上以出芽方式获得囊膜而成热。     

禽腺病毒江苏分离株的细胞培养特性

为进一步研究的生物学特性,应用鸡胚成纤维细胞(CEF)和鸡胚肾细胞(CEK)培养禽腺病毒江苏分离株,进行培养特性、血凝特性、理化特性、超薄切片观察和PCR检测。实验结果表明,CEF感染病毒后没有出现病变。CEK感染病毒后则表现出很强的聚集性并形成细胞团,有的成束状,最后脱落;Reed-Muench方法测得其TCID50为每0．1mL 10^-5.4。CEK核内存在大量70～80nm、呈典型的晶格状排列的腺病毒颗粒。     

Effect of Scheduling Irrigation at Different Phenophases of Sweet Corn (Zea mays saccharata) and Delineation of Critical Stages Based on Stress Day Index

A field experiment was conducted during the summer of 1996 at the Main Research Station, University of Agricultural Sciences, Hebbal, Bangalore to study the effect of phenophased irrigation schedules on green cob and fodder yield and to delineate critical stages based on Stress Day Index. There were seven irrigation schedules consisting of a combination of two IW/CPE ratios (irrigation water/cumulative pan evaporation), viz. 0.6 and 0.8 shuffled at three growth stages: 10–35 DAS (days after sowing), 36–65 DAS and 66 days to harvest in RCBD (randomized complete block design) with four replications. The investigations revealed that all yield attributing characters such as cobs per plant, cob weight, green cob and fodder yields were significantly higher under the irrigation schedule of 0.8 IW/CPE ratio throughout the crop growth. The reduction in green cob and fodder yields were 39 and 25 % respectively, when irrigations were scheduled at 0.6 IW/CPE ratio throughout as compared to 0.8 IW/CPE. Also, frequent irrigation at 0.8 IW/CPE ratio throughout maintained higher plant relative water content (86.90 %) and higher available soil moisture (59.95–81.51 %) before irrigation as compared to delayed irrigations at 0.6 IW/CPE ratio. The quality parameter viz. sucrose content (2900 μg g−1) in developed seeds was also higher under frequently irrigated treatment. The stress day index indicated that vegetative followed by silking and tasseling were the critical stages for moisture stress.     

PRRSV青海株与其他疫苗株对Marc-145细胞的毒力比较

[目的]研究青海PRRSV毒株对Marc-145细胞的适应性和增殖速率以及致病性和毒力等.[方法]以PRRSV青海毒株QH08、海利和诺倍威CH-1R经典毒株、GW-HR毒株及其在Marc-145细胞上的传代毒株为研究对象,对4种毒株及其传代毒株的TCID50值和细胞病变作用(CPE)出现的时间进行比较,探讨了PRRSV青海毒株对Marc-145细胞的适应性、在Marc-145细胞内的增殖速率以及该毒株的毒力.[结果]随着PRRSV传代次数的增加,病毒在Marc-145细胞中的增殖速率加快,对细胞的适应性和毒力都增强.与其他3个毒株相比,青海株毒力最强;C PE出现的时间提前,说明病毒在Marc-145细胞中的复制周期缩短,表明不同毒株PRRSV的F10比F1在Marc-145细胞中具备更好的复制能力和致病性.[结论]4株PRRSV传代毒10个代次的TCID50总趋势为渐变上调的趋势,CPE出现的时间总体上呈逐渐缩短的趋势.     

Growth characteristic of Camel pox and Vaccinia viruses in embryonated eggs and cell culture

Camel pox viruses isolated in Sudan and VD45 (African camel pox strain) and Vaccinia virus (Elstree strain) were used for inoculation of chorioallantoic membrane (CAM) of embryonated eggs (EE) and cell culture (CC). In EE Lesions were seen as pocks ranging in size from 1 to 1.5 mm in diameter, and they increase in size with serial passage and taking opaque- white and opaque- yellow colors. When propagated in Vero cells, these viruses gave clear CPE, characterized by rounding of cells, plaque formation, syncytia and detachment of cells from the glass.     

猪繁殖与呼吸综合征毒株在Vero细胞上的增殖验证试验

为验证猪繁殖与呼吸综合征病毒在Vero细胞上的增殖,进一步规范非禽源外源病毒检验操作,将目前国内用于疫苗生产的5个猪繁殖与呼吸综合征病毒毒株分别在Vero细胞上继代．观察细胞病变并进行荧光抗体染色。结果显示,国内5个猪繁殖与呼吸综合征病毒毒株均能在Vero细胞上增殖,且能引起细胞出现圆缩、聚集和脱落等典型的PRRSV细胞病变,在细胞胞浆内也可观察到特异性绿色荧光。结果提示在进行猪繁殖与呼吸综合征活疫苗外源病毒检验时,样品须进行特异性血清中和处理．以赍,干扰外源病毒枪蛤结集垧判常     

采用继代鸡胚成纤维细胞生产鸡传染性法氏囊细胞苗的试验

采用原代鸡胚成纤维细胞和继代鸡胚成纤维细胞,生产了鸡传染性法氏囊细胞苗,效价(TCID50/mL)无明显差异.采用继代鸡胚成纤维细胞生产鸡传染性法氏囊细胞苗,减少种蛋消耗,降低劳动强度,增加产量,降低生产成本.     

反义RNA介导的抗猪传染性胃肠炎病毒感染

构建了重组逆转录病毒表达质粒plxas-N,该质粒能表达互补猪传染性胃肠炎病毒N基因全长的反义RNA序列。用质脂体方法将重组质粒plxas-N转染至PA317细胞中,经抗生素G418（500μg/ml）筛选出稳定的产毒细胞克隆。分别扩大培养细胞克隆,取其上清液感染小鼠成纤维细胞NIH3T3,测定细胞克隆产生的重组病毒滴度,并用高滴度重组病毒感染IBRS2细胞。提取被感染的IBRS2细胞的总RNA,RT-PCR证明plxas-N整合到IBRS2细胞基因组。通过TGEV分别感染IBRS2和IBRS2抗性细胞产生的病变,结果表明该反义RNA对病毒的复制有抑制作用,其抑制率近50%。     

云南香猪PRRSV分离株ORF7基因遗传变异分析

为了解2014年云南省香猪群体中猪繁殖与呼吸综合征病毒(PRRSV)的流行特征,将疑似猪繁殖与呼吸综合征(PRRS)病例肺脏和淋巴结研磨处理后接种Marc-145细胞进行盲传,传至第2代48 h时出现2株病毒致Marc-145 细胞聚集融合、巨细胞等病毒致细胞病变(CPE)效应现象,将其命名为YN-2XZ1和YN-3X...     

两株广西伪狂犬病病毒的分离鉴定

从广西不同地方的发病仔猪脑内分离到两株病毒,分别命名为Ｂ株和Ｗ株。用这两株病毒分别接种家兔,产生的症状和病变与伪狂犬病病毒强毒ＭｉｎＡ株相似。将两株病毒在乳兔肾细胞和ＣＥＲ细胞上连续传代,均稳定地出现与ＭｉｎＡ株相同的细胞病变（ＣＰＥ）。在ＣＥＲ细胞上进行微量血清和试验,ＭｉｎＡ株的抗血清能完全中和Ｂ株和Ｗ株对细胞的致病变作用,表明两个毒株与ＰｒＶ强毒具有相似的抗原性。电镜下观察Ｂ株和Ｗ株病毒粒子