免疫增强剂TMFN对犬外周血CD4/CD8比值的影响

采用检测犬外周血 CD4和 CD8比值变化的方法 ,观察犬注射免疫增强剂 TMFN和犬疫苗后 ,不同组之间的 CD4和 CD8比值 ,以证明该免疫增强剂对犬免疫系统的影响。结果表明 ,该免疫增强剂能够提高正常犬的细胞免疫水平 ,在犬注射疫苗的同时应用该免疫增强剂 ,可显著提高疫苗的免疫效力     

Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop

Among the 57 monoclonal antibodies (mAb) analyzed within the T-cell group from the Second Swine CD Workshop, six mAb fell within clusters T10 and T11 (No. 088, STH164; No. 148, FY1A3; No. 149, FY2C1; No. 150, FY1H2; No. 151, FY2A11; No. 169, BB23-8E6). The mAb within these two groups gave a similar appearance on flow cytometry and stained all peripheral blood T-cells as defined by CD4 and wCD8 staining. All six mAb precipitated a 24 kDa protein. On the basis of inhibition analyses performed as part of the workshop and from published data, the mAb define at least three epitopes. There is only minimal stimulation of resting peripheral lymphocytes, but four of the mAb produce strong stimulation in the presence of PMA. With the exception of STH164, all have been shown to react with CD3-transfected COS cells. The new mAb, therefore, react with three epitopes on porcine CD3 designated CD3a (BB23-8E6, FY2A11), CD3b (FY1A3, FY2C1), and CD3c (FY1H2). mAb STH164 appears to be reactive with another epitope, however, since its reactivity with CD3 has not been confirmed it is designated as wCD3.     

传染性支气管炎病毒结构蛋白免疫鸡对CD4+CD8+T淋巴细胞亚群的影响

为了比较传染性支气管炎病毒3种主要结构蛋白免疫鸡后对CD4+、CD8+T淋巴细胞亚群的不同影响.本试验以pVAX1载体为携带工具,制备分别含有IBV主要结构蛋白基因的质粒免疫健康雏鸡,采用流式细胞仪(FACS)对免疫鸡外周血中CD4+、CD8+T淋巴细胞数进行检测.结果显示:各试验组间,携带N蛋白基因的质粒在免疫后3周内CD4+T淋巴细胞数和CD8+T淋巴细胞数均高于其他组,且差异极显著(P＜0.01).由此可见,IBV的N蛋白可明显诱导CD8+T淋巴细胞的CTL免疫作用和CD4+T淋巴细胞的辅助性免疫作用,其细胞免疫原性高于S1蛋白和M蛋白.     

鸡CD4蛋白基因的克隆及其mRNA在淋巴器官中的表达

从鸡胸腺、脾脏、哈德氏腺、外周血液和法氏囊等免疫器官中分离淋巴细胞,应用RT-PCR对这些免疫器官中鸡CD4分子mRNA的表达进行了研究。同时,用RT-PCR对鸡脾淋巴细胞CD4分子cDNA进行了克隆和序列测定。结果表明,在上述器官中,法氏囊淋巴细胞不表达鸡CD4分子mRNA,其他器官中均有表达。通过序列分析表明,本试验扩增得到的基因为编码鸡CD4分子的基因。     

高校图书馆附书光盘网络化管理的实践探讨

本文提出了高校图书馆附书光盘网络化管理模式的,必要性,以湖南农业大学图书馆为例,对光盘网络化管理实践进行了全面地探讨.     

宁麦9号与扬麦158株高及其构成因素的遗传解析

宁麦9号与扬麦158是我国长江中下游麦区的主栽品种和骨干亲本,长江中下游麦区近3年来审定品种中80%都是其衍生后代,研究其性状的遗传具重要意义。以宁麦9号与扬麦158为亲本构建的包含282个家系的重组自交系群体为材料,利用Illumina 90k芯片对群体进行基因型分析,建立高密度遗传图谱。连续3个生长季对株高及节间长度、穗长等株高构成因素进行测定,结合遗传图谱对株高及相关性状进行QTL定位,获得14个控制株高及其构成因素的稳定表达位点。通过进一步位置比对,聚焦到6个染色体区段,初步明确了各节间对株高的遗传调控机制。同时,将6个染色体区段中同源性较低的连锁标记转化为适用于高通量筛选的KASP标记,利用101份区域试验材料进行标记效应验证,结果显示聚合Qph-2D与Qph-5A.1两个位点具有较高的选择效率,继续聚合Q2A后,中选材料显著减少,可能降低选择效率;对Q2A与Q5A两个一因多效位点的选择建议以降低株高的等位变异为主;Qd1-5D可作为穗下节间(D1)的选择标记对株高展开优化选择。期望以上结果能为长江中下游麦区的小麦株高遗传改良提供帮助。     

Anti-diabetic effects of emodin involved in the activation of PPARγ on high-fat diet-fed and low dose of streptozotocin-induced diabetic mice

Rheum palmatum Linn has been widely applied in the clinical treatment of diabetes mellitus. It has been found that emodin as the major bioactive component of R. palmatum L exhibits the competency to activate peroxisomal proliferator-activated receptor-γ (PPARγ) in vitro. So the aim of this study was to evaluate the anti-diabetic effects of emodin through the activation of PPARγ on high-fat diet-fed and low dose of streptozotocin (STZ)-induced diabetic mice. The diabetic mice were intraperitoneally injected with emodin for three weeks. No changes of food consumption and the body weight in emodin-treated mice were monitored daily during the entire experiment. At the end of experiment, the levels of blood glucose, triglyceride and total cholesterol in serum were significantly decreased after emodin treatment. However, serum high-density lipoprotein cholesterol (HDLc) concentration was significantly elevated. The glucose tolerance and insulin sensitivity in emodin-treated group were significantly improved. Furthermore, the results of quantitative RT-PCR analysis showed that emodin significantly elevated the mRNA expression level of PPARγ and regulated the mRNA expressions of LPL, FAT/CD36, resistin and FABPs (ap2) in liver and adipocyte tissues. No effects on the mRNA expressions of PPARα and PPARα-target genes were observed. Taken together, the results suggested that the activation of PPARγ and the modulation of metabolism-related genes were likely involved in the anti-diabetic effects of emodin.     

CD31可作为分离纯化猪肝窦内皮细胞的标记分子

血小板-内皮细胞粘附分子(platelet endothelial cell adhesion molecule-1,CD31)是内皮细胞表面一种重要的膜分子,常作为特异性标记鉴定内皮细胞.然而,CD31是否可作为纯化猪(Sus scrofa)肝窦内皮细胞(porcine liver sinusoidal endothelial cell,pLSEC)的特异性标记还有待验证.本实验通过CD31流式分选分离纯化pLSEC,观察分离后的pLSEC是否具有标志性的窗孔结构.以α-1,3-半乳糖苷转移酶基因敲除(α-1,3-galactosyltransferase knockout,GTKO)的五指山小型猪为研究材料,采用胶原酶原位肝脏灌注、离体消化,Percoll梯度离心,异硫氰酸荧光素(fluorescein isothiocyanate,FITC)-CD31标记pLSEC进行流式分选.结果表明,分离得到的pLSEC在倒置显微镜下细胞呈典型的内皮细胞形态,铺路石样排列;通过透射电镜观察到细胞具有窗孔结构,通过环境扫描电镜观察到pLSEC与猪主动脉内皮细胞(porcine arterial endothelial cell,pAEC)细胞表面结构具有明显区别,pLSEC具有特殊的窗孔结构.利用实时荧光定量PCR检测pLSEC、pAEC和猪耳成纤维细胞(porcine ear fibroblast cell,pEFC)的CD31、细胞粘附因子(cell adhesion molecules,CD146)、血友病因子Ⅷ基因(hemophilia factorⅧ,Ⅷ-F)、血管性血友病因子基因(von willebrand factor,vWF)的表达.结果表明,pLSEC的CD31、CD146、Ⅷ-F和vWF表达量显著高于pAEC和pEFC.利用免疫荧光法检测到pLSEC具有摄取细胞膜红色荧光探针(1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate,DiI)标记的乙酰化低密度脂蛋白(acetylated low density lipoprotein,Ac-LDL)的内吞功能.总之,利用CD31分离纯化的pLSEC,具有典型窗孔结构和内吞功能,同时高表达CD146、Ⅷ-F和vWF等肝窦内皮细胞的标记分子,为进一步研究异种肝移植的血小板减少症的问题提供了良好的细胞材料.     

对高校图书馆随书光盘管理的探讨

本文从我馆对随书光盘管理模式经历的3大阶段进行探讨,建议从4大方面做好随书光盘的管理与应用:一是严格把守光盘入库的质量关,二是大力提升馆员的技术素质,三是培养读者对光盘的正确使用习惯,四是采用适合各馆的随书光盘下载管理系统.     

杜仲梦尼夜蛾CD36家族同源基因克隆与组织分布研究

目的 揭示杜仲梦尼夜蛾CD36家族同源基因神经元膜蛋白(SNMPs)和b族清道夫受体(SRb)的序列特征和组织分布情况。 方法 设计特异性引物克隆杜仲梦尼夜蛾SNMPs和SRb基因,对这些基因编码的蛋白进行同源建模;通过荧光定量PCR分析这些基因在不同组织的表达情况。 结果 在杜仲梦尼夜蛾中鉴定得到3个CD36家族同源基因（OsonSNMP1、OsonSNMP2和OsonSRb1）;这些基因编码的蛋白具有2个跨膜区域和1个细胞外结构域。空间结构预测发现,这些蛋白的胞外结构域包含1个主要由反向平行β-折叠构成的桶状核心和1个富含疏水性氨基酸残基的α-螺旋;荧光定量PCR结果表明,OsonSNMP1、OsonSNMP2和OsonSRb1均在触角中大量表达;OsonSNMP1在雄虫触角中的表达水平显著高于雌虫触角,OsonSNMP2在雌虫触角的表达水平显著高于其他组织,OsonSRb1在雄虫触角中大量表达外,还在雌雄虫的口器中大量表达。 结论 本研究发现OsonSNMP1、OsonSNMP2和OsonSRb1编码的蛋白具有与脊椎动物CD36家族受体类似的空间结构,与嗅觉器官高度关联的表达模式表明这些基因可能在杜仲梦尼夜蛾嗅觉系统中发挥了功能,以上结果为探究杜仲梦尼夜蛾CD36家族同源基因的嗅觉功能提供了数据。