同一基础材料的玉米双单倍体(DH)系配合力的分析

利用高频玉米单倍体诱导系,对选系材料M35/F35的F1代进行诱导获得大量单倍体籽粒,经自然加倍获得一批纯合双单倍体(DH)系,用测验系京24组配杂交组合分析DH系的配合力表现。结果表明,来源于同一基础材料的不同DH系之间,配合力差异较大,部分DH系的配合力比亲本增加,说明采用单倍体育种可以选育到高配合力的优良玉米自交系,组配出优良品种,从而加快育种进程,应用前景广阔。     

卵巢癌患者血清可溶性CD44、CA125和TSGF水平的检测及临床意义

目的观察卵巢癌患者手术治疗前后血清中sCD44、CAl25和TSGF水平变化及临床意义。方法分别应用单克隆夹心EusA、放射免疫分析和化学发光法定量检测46例患者手术前后血清sCD44、CAl25和TSGF水平,并与50例健康体检者比较。结果46例患者中卵巢癌Ⅲ／IV期患者血清sCD44、CA125和TSGF水平均显著高于卵巢癌I／II期患者,卵巢癌Ⅱ期患者仅CAl25水平高于卵巢癌I期患者;在手术治疗前血清sCD44、CAl25和TSGF水平均高于正常对照组（P〈0．01）,经手术治疗6个月后则与正常对照组比较差异无统计学意义（P〉0．05）,且卵巢癌患者血清sCD44水平与CAl25和TSGFT水平呈正相关（r=0．7256、0．5764,均P〈0．01）。结论检测血清sCD44、CA125和TSGF水平的变化对卵巢癌的早期诊断、术后疗效观察和预后评估均有重要的临床意义。     

8-羟基喹啉和柠檬酸对切花菊生理效应的影响

以不同浓度8-羟基喹啉(8-HQ)和柠檬酸(CA)进行组合作为保鲜液,通过外部形态观察和生理指标测定,研究保鲜剂对切花菊的保鲜效果。结果表明:8-HQ和CA对延缓切花菊的衰老有明显作用,可延长其瓶插寿命,增加花枝鲜重,减缓花瓣组织中蛋白质和糖含量的分解速度。处理A 3%蔗糖+100 mg/L 8-HQ+200 mg/L CA保鲜效果最好,可使切花菊的瓶插寿命比对照延长10 d。     

牦牛病毒性黏膜病病毒P125基因的克隆及序列分析

试验参考GenBank中发表的牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)毒株基因组序列设计了5对引物,利用PCR扩增出预期的3475 bp目的片段;将扩增产物连接至pMD19-T 载体,经质粒PCR鉴定及双酶切鉴定获得阳性重组质粒并进行核苷酸序列测定。经BLAST同源性分析表明,牦牛BVDV P125基因与BVDV-Ⅰ型的NADL毒株P125基因的同源性为63.4%。系统分析结果表明,牦牛P125基因与BVDV标准毒株P125基因在遗传进化与亲缘关系上均较远,这可能是因为环境诱变的结果或该毒株具有新的遗传衍化来源。     

基于CA的IPSec实现框架

基于身份验证CA(Certificate Authority),给出了包括IPSec通道启动、IPSec通道包处理过程、IPSec安全策略刷新的IPSec实施过程和框架.     

A REVIEW OF PERMANENT INTERSTITIAL IMPLANT RADIOTHERAPY USING RADON-222 AND IODINE-125*

It is important that veterinarians who perform radiation therapy possess a minimum basic knowledge of interstitial implant radiotherapy and of radionuclides used as interstitial implants. The advantages and disadvantages of interstitial implants as compared with tele-therapy, surgery, and chemotherapy, are presented. Two radionuclides, radon-222 and iodine-125, are discussed, and their physical parameters, cost, safety, and radiotherapeutic efficacy in human patients are compared. Three methods of dosimetry are briefly discussed: the Patterson-Parker system, the Quimby system, and the dimension averaging technique. Some types of implant needles and associated implant equipment are described. It is concluded that permanent interstitial implants deserve consideration as a modality for treatment of appropriate spontaneous animal neoplasms.     

优质长粒粳稻恢复系C470及其改良利用

从培矮645/轮廽422组合选育的C470,谷粒长宽比3∶1,株叶形态偏籼,育性亲和性与米质为粳型,垩白率和垩白度可达国优一级,配组优势和品质能同步提高,可以作为IR24质型粳稻恢复系和优质亲本利用,其恢复谱窄,不耐寒等缺点正在改良中。     

气调箱贮藏甜樱桃品质变化研究

本文以美早和萨米脱为试材,研究了气调箱保鲜处理对果实贮藏品质变化的影响。结果显示,甜樱桃贮藏过程中,气调箱内CO2浓度基本维持在12-15%;气调箱处理甜樱桃果实硬度显著高于对照果实,轻微降低了可溶性固形物的含量;但却保持了果实较高的可滴定酸含量以及维生素C含量。气调处理最明显的效果就是降低了甜樱桃果实的腐烂率。两品种相比较,萨米脱的腐烂率要远低于美早,耐贮性较好。两品种果实0oC贮藏60 d后仍具有较好的感官品质,并可以保证3 d的常温货架期。     

A modified chemiluminescence method for hydrogen peroxide determination in apple fruit tissues

Hydrogen peroxide (H₂O₂) is one of the important by-products produced by plant and fruit tissues during normal metabolism as well as under stress conditions. Evidence suggests that it is actively involved in many physiological activities in plants, including ripening, senescence and the development of disorders. Quantitative measurement of H₂O₂ in fruit has been a challenge due to variations in methodologies, and their sensitivities and interferences present in plant samples. Among the currently used methodologies, chemiluminescence (CL) is one of the most promising, due to its high specificity and sensitivity. However, direct application of CL methods developed for leaf analysis is not suitable for fruit, especially fruit peel tissues, possibly due to interfering compounds in fruit tissues. In this study, evaluation of the efficiency of removal of interfering compounds by PVP, PVPP and activated charcoal revealed that the PVPP is the most effective compound to remove the interference. This modified protocol can measure H₂O₂ content in apple peel and flesh tissues. ‘Red Delicious’ apple peel and flesh tissues were measured with amount of 1.48 and 1.03 μmol/g FW, respectively. The established protocol can also be used for a wide variety of tissues in addition to apple fruit, including strawberry tissues (fruit, calyx and leaves) and spinach leaves. This protocol was applied to determine the H₂O₂ concentration in 1-MCP and DPA treated apples after 5 months of storage, but no significant difference in H₂O₂ in those samples was found. Direct comparison of CL with a commercial hydrogen peroxide measurement OXIS kit was also made. The challenges to accurately assay H₂O₂ in fruit/plant tissue were discussed.