Improvement bread characteristics of high level sunn pest (Eurygaster integriceps) damaged wheat by using transglutaminase and some additives

The purpose of this study was to improve the baking quality of high level sunn pest damaged wheat (HLSPDW; 20.6%) sample by using at varying levels transglutaminase, and fixed additive combination (diacetyl tartaric acid esters of mono and diglycerides + citric acid + L-ascorbic acid) with or without transglutaminase. It was observed that transglutaminase plays an important role in baking quality of HLSPDW. The increase in transglutaminase caused very clear increase on bread characteristics of wheat. Bread yield, height, pore structure, and crumb softness values increased sharply; weight loss and wideness of bread samples decreased accurately depending on increasing transglutaminase level. This increase did not affect obviously on bread quality at a certain proportion (0.3%). However, when the transglutaminase was more than 0.3% and depend on increase of percentage, bread characteristics showed significant increase. It was determined that, in the absence of transglutaminase; other additives could not improve the bread qualities examined. The unique application of using transglutaminase was found to be considerably to improve the bread quality of the HLSPDW. Overall results indicate that the properties of the bread from HLSPDW can be restored by the addition of transglutaminase. The highly disrupted protein structure present in the HLSPDW gluten requires higher transglutaminase concentrations.     

碳酸盐碱度胁迫对尼罗罗非鱼血清pH、游离氨浓度及相关基因表达的影响

【目的】了解碳酸盐碱度对罗非鱼的毒性及其相应耐受机制,为耐盐碱罗非鱼育种及生产应用提供理论指导。【方法】将尼罗罗非鱼从淡水直接转入4个碳酸盐碱度处理(4.0、6.0、8.0和10.0 g/L NaHCO3)中进行急性胁迫试验,利用pH计、紫外分光光度计及荧光定量PCR,分别检测胁迫0.5、1.0、4.0、6.0、9.0、12.0、18.0、24.0、36.0和48.0 h的罗非鱼血清pH、游离氨浓度及肾组织中碳酸酐酶(CA)和谷氨酰胺合成酶(GS)基因的相对表达量。【结果】在各碳酸盐碱度处理组中,胁迫1.0 h后罗非鱼的血清pH、游离氨浓度均发生显著变化(P<0.05)。经4.0和6.0 g/L碳酸盐碱度胁迫48.0 h后未出现死鱼现象,其血清pH、游离氨浓度及CA基因、GS基因的表达量均呈先升高再降低的变化趋势;经8.0 g/L碳酸盐碱度胁迫9.0 h后陆续出现死鱼现象,血清pH高达8.11、游离氨浓度为486.28μmol/L,至胁迫12.0 h后罗非鱼全部死亡;经10.0 g/L碳酸盐碱度胁迫出现死鱼的时间明显早于8.0 g/L碳酸盐碱度胁迫,胁迫1.0 h后其血清pH达8.14、游离氨浓度为365.89μmol/L,胁迫4.0 h后罗非鱼全部死亡。【结论】尼罗罗非鱼能耐受的血清pH上限在8.10附近,耐受的游离氨浓度为525.66μmol/L。在低碳酸盐碱度(小于6.0 g/L)胁迫下,尼罗罗非鱼可通过生理调控血清pH和游离氨浓度而适应胁迫逆境,且CA和GS参与了相应离子的转运及氨氮代谢。     

miR-145-4调控蛋鸭卵泡发育机制的研究

【目的】本研究旨在揭示miR-145-4在蛋鸭卵泡发育中的作用及调控机制。【方法】分离培养蛋鸭卵泡颗粒细胞,转染miR-145-4相似物(mimic)、抑制物(inhibitor)后,利用实时荧光定量PCR法检测细胞增殖凋亡相关基因的表达情况,采用RNAhybrid和Targetscan程序预测miR-145-4的靶基因及其与靶基因磷脂酰肌醇-3-激酶催化亚单位α(phosphatidylinositol-3-kinase catalytic subunitα,PIK3CA)3′-UTR的结合位点,分别构建PIK3CA基因3′-UTR的野生型和突变型双荧光素酶载体,与miR-145-4 mimic和mimic-NC共转染蛋鸭卵泡颗粒细胞后,采用双荧光素酶报告系统验证miR-145-4与靶基因PIK3CA的结合关系,最后采用实时荧光定量PCR法检测miR-145-4对蛋鸭卵泡颗粒细胞中PIK3CA基因表达的影响。【结果】实时荧光定量PCR结果显示,与对照组相比,过表达miR-145-4后CyclinB2基因的表达量极显著降低(P<0.01),BCL2基因的表达量极显著增加(P<...     

miR-125a-5p suppresses epithelial-mesenchymal transition via GSK-3β/Snail signaling pathway in breast cancer cells

AIM: To investigate the function of microRNA-125a-5p (miR-125a-5p) on epithelial-mesenchymal transition (EMT) of breast cancer cells via GSK-3β/Snail signaling pathway.METHODS: The expression of miR-125a-5p in normal breast epithelial cells and breast cancer cells, as well as the transfection efficiency of miR-125a-5p plasmid in MDA-MB-231 cells was detected by RT-qPCR. The chemotaxis ability and invasion ability were detected by chemotaxis assay and Transwell invasion assay. The changes of EMT-related markers, the protein level of phosphorylated glycogen synthase kinase-3β (p-GSK-3β) and the nuclear translocation of Snail were determined by Western blot. RESULTS: The expression of miR-125a-5p in the breast cancer cells was significantly lower than that in the normal breast epithelial cells. The expression of miR-125a-5p was significantly higher in MDA-MB-231/miR-125a-5p cells than that in MDA-MB-231/NC cells. The ability of epithelial growth factor (EGF) at 10 μg/L to induce chemotaxis of MDA-MB-231 cells was the strongest. Compared with MDA-MB-231/NC group, stimulation of EGF decreased the invasion ability of MDA-MB-231/miR-125a-5p cells, and resulted in the increase in E-cadherin expression, while significantly decreased the protein levels of vimentin and p-GSK-3β. Meanwhile, the nuclear localization of Snail was significantly inhibited. The invasion capacity of MDA-MB-231/miR-125a-5p+GAB2 cells was significantly enhanced compared with MDA-MB-231/miR-125a-5p+Con cells, the expression of E-cadherin was decreased, and the protein levels of vimentin and p-GSK-3β were significantly increased, while the nuclear localization of Snail was promoted. CONCLUSION: miR-125a-5p suppresses EMT via GSK-3β/Snail signaling pathway, thus inhibiting the invasion ability of breast cancer cells.     

高大平房仓整仓富氮低氧气调防治储粮害虫研究

富氮低氧气调储粮是一项无公害、无污染、“零排放”的绿色储粮新技术,是近年来绿色生态储粮技术的主要手段和发展方向.良好的仓房气密性是气调储粮技术得以成功运用的关键,本次研究实验仓房粮面没有采用薄膜压盖密闭处理,直接进行整仓气调作业,并使氮气浓度达到98％以上,维持一定时间,通过对所得实验数据的分析,结果表明整仓富氮低氧气调具有较好的实用性,对锈赤扁谷盗和谷蠹均有明显杀灭效果,而且整仓气调可以解决膜下富氮低氧气调工艺存在的空间防治问题,并减少劳动强度.     

氮磷钾钙元素对果树生长的影响及施用建议

氮、磷、钾、钙作为果树生长必需的营养元素,对果树的生长发育、果实产量和品质、抗寒性、抗旱性等都有重大影响。本文对氮、磷、钾、钙元素对果树生长的影响及各类元素的施用建议进行了阐述,以期为果农科学有效施肥、提高果品质量提供参考。     

血清CA125、CA19-9、HE4联合检测在卵巢癌诊断中的应用价值研究

目的 :探讨联合检测血清CA125、CA19-9、HE4在卵巢癌诊断中的应用价值。方法:收集卵巢癌标本70例(卵巢癌组)、卵巢良性病变标本36例(良性病变组)、健康妇女卵巢标本42例(正常对照)。对研究对象进行血清CA125、CA19-9及HE4的检测,比较三组的血清CA125、CA19-9及HE4的水平及阳性检出情况,对比三种肿瘤标记物在卵巢癌诊断的敏感性、特异性。结果 :卵巢癌组、良性病变组CA125、CA19-9、HE4水平明显高于对照组(P<0.05),卵巢癌组明显高于良性病变组(P<0.05)。卵巢癌组、良性病变组CA125、CA19-9阳性率明显高于对照组(P<0.05);卵巢癌组CA125、CA19-9、HE4阳性率均明显高于良性病变组(P<0.05)。单项检测中,CA125敏感性最高,其次为HE4;HE4特异性最高,其次为CA19-9。两项联合检测中,CA125+HE4敏感性最高,为94.2%,CA19-9+HE4特异性最高为87.9%,三项联合检测的敏感性和特异性分别为97.1%、79.5%。三项联合检测敏感性明显高于单项检测和两项联合检测(P<0.05)。结论:CAl25、CAl9-9、HE4联合检测可明显提高对卵巢癌诊断的敏感性。     

销售条件对气调贮藏的旭和元帅苹果品质下降速率的影响

在低乙烯气调贮藏和模拟销售条件下对旭和元帅苹果品质下降的速率进行了研究。在2%O_2+3%CO_2+95%N_2的气调条件下贮藏0,2,4,6和8个月后分为5组,分别放在20℃,10℃或以12小时交替的0°和20℃下1～14天(旭)或1～20天(元帅)。结果表明,旭和元帅苹果的硬度、含酸量在气调贮藏期间逐渐下降,销售期间两者损失的速率在20℃下比在10℃下或以12小时交替于0°和20℃下的快一倍;贮藏和销售期间的可溶性固形物含量变化不大;气调贮藏时间对旭和元帅苹果在销售期间硬度下降速率的影响表现出不同的模式。     

PT125TM蛋白酶对宁都三黄鸡的影响

选用健康的1日龄三黄鸡1200羽(公母各半),初生重接近,随机分为4组,每组5个重复,每个重复60羽(公母各半),分别为A1、A2、A3、A4。A1组饲喂常规蛋白质水平日粮,A2组饲喂低蛋白水平日粮(0～4周蛋白质含量低3%,5～12周蛋白质含量低5%),A3组日粮在A1组日粮的基础上添加125mg/kg的PT125TM,A4组日粮在A2组日粮的基础上添加125mg/kg的PT125TM。分别在4周龄、8周龄、12周龄三个阶段测定宁都三黄鸡十二指肠内容物淀粉酶和肌胃内容物蛋白酶活性、免疫器官指数、日增重、日采食量、饲料转化率,在6周龄和12周龄测定宁都三黄鸡日粮干物质消化率和氮的表观消化率、血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,在6、8、10、12周龄时测定血清中总蛋白和球蛋白含量。结果表明:PT125TM蛋白酶可提高宁都三黄鸡干物质消化率和氮的表观消化率,提高宁都三黄鸡的饲料转化率,特别是在生长发育期效果显著;提高三黄鸡抗热氧化能力,降低由于热应激造成的生产性能损失;提高蛋白质消化率,降低日粮蛋白质水平,节约蛋白质资源。     

碳分子筛气调贮藏猕猴桃的研究

用６～２．２％Ｏ２＋０～３．７％ＣＯ２和６～１％Ｏ２＋０～５％ＣＯ２进行猕猴桃碳分子筛气调贮藏，能明显地延缓果实的淀粉和原果胶的转化，降低Ｖｃ的损失。贮２７周，果实仍能保持８．５３ｋｇｆ／ｃｍ２的硬度。ＣＡ贮藏效果好于ＭＡ贮藏和０℃冷藏。两种气调贮藏的贮藏效果基本相同