罗汉果生产技术研究的思路与发展对策

探讨了罗汉果生产技术研究思路，提出选育、引进优良品种，培育茎尖脱毒壮苗，建立种薯基地，采用容器无土栽培、平地栽培、设施栽培，施用天敌真菌、接种VA菌根真菌防治根结线虫等技术建议。     

中国香瓜与菜瓜地方品种资源白粉病抗性评价

春秋2个季节在温室利用风媒接种方法对搜集的102份香瓜和14份菜瓜种质资源进行了白粉病抗性评价,结果鉴定出7份香瓜和1份菜瓜高抗白粉病,其病情指数为零,1份菜瓜抗病,其病情指数0.9,表明我国香瓜和菜瓜地方品种资源中蕴涵着潜在的改良甜瓜白粉病抗性的基因资源。     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

Study on relationship between expression of PKCα in glomeruli and development of nephropathy in diabetic rats

AIM:To observe the dynamic changes of expression of PKCα, TGF-β₁ and α-SMA in glomeruli of diabetic rats induced by the alloxon and to invesitigate their roles in the diabetic nephropathy(DN).METHODS:Rats were randomly divided into four groups: normal control group (group A), diabetic group of one week (group B), diabetic group of one month (group C), diabetic group of two months (group D). Immunohistochemistry and Western blotting were used to detect the expression of PKCα, TGF-β₁ and α-SMA in renal tissue of all groups. Blood glucose, triglycerides, cholesterol, creatinine and urine protein were analysed by chemical methods. The morphological changes of renal tissue were checked through microscopy.RESULTS:The expression of PKCα and TGF-β₁ in renal tissue of diabetic groups were increased comparing with those of nomal control group(P<0.05). The mesangial cells expressed α-SMA in two months group. Chronologically the expression of PKCα, TGF-β₁ and α-SMA were positively correlative with each other and the impairment of kidney was also observed.CONCLUSIONS: During the DN process the expression of PKCα increased. PKCα raised GFR and the permeability of glomerular filtration membrane which enhanced urinary albumin excretion. PKCα also increased expression of TGF-β and therefore to induce the expression of α-SMA. The appearance of α-SMA was a marker of the phenotypic transform of renal cells.     

台湾樟芝对碳素营养源利用的研究

本文报道了台湾樟芝 (Antradiacomphora)液体深层培养过程中 ,六种不同碳素营养源组成的合成液体培养基 ,对樟芝菌球生长的影响 ,结果表明 ,樟芝对六种碳源均可利用 ,其中以麦芽糖利用效果最佳 ,菌球数量及菌丝体干重均居首位 ,菌球数量是对照的 3 5 5倍 ,菌丝体干重比对照高出1 73倍 ,樟芝对乳糖的利用较差 ,菌球数量虽比CK仅少 2 5个 / 10 0mL ,但菌体干重相差较大 ,每10 0mL相差 2 5 6 9mg。对樟芝发酵液pH测定情况看来 ,不同碳源组成的培养基终止pH表现不同 ,以麦芽糖、葡萄糖、果糖、蔗糖为碳源的培养基终止pH为 4 5～ 5 0左右。甘露醇为碳源的培养基终止发酵pH为 3 5～ 4 0 ,而乳糖则为 5 5以上。试验表明 ,樟芝菌丝生长极其缓慢 ,在液体深层培养过程中 ,若培养基配方不合理 ,菌球就不生长 ,本文筛选了适合樟芝深层培养的碳素营养源 ,为樟芝的开发和利用提供了科学依据     

Effects of protein kinase C on modulation of vascular endothelium permeability

Protein kinase C pathway is an important intracellular signal transduction pathway. A growing number of evidences showes that activation of PKC influences endothelial cell permeability. In this review, we briefly summarize the effects and regulating pathways of protein kinase C in modulation of vascular endothelium permeability.     

替米考星治疗人工感染巴氏杆菌病仔猪试验

研究了国产替米考星注射用药对人工感染多杀性巴氏杆菌病猪的治疗效果,用断奶仔猪进行试验,为临床使用提供了依据.结果表明,替米考星治疗急性猪肺疫效果确实, 推荐剂量为10 mg/kg体重.     

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

秀丽纤杆线虫在寄生性线虫抗原研究中的应用

秀丽纤杆线虫 (Caenorhabditis elegans)是一种模式线虫 ,目前广泛应用于寄生性线虫基因的功能研究 ,并且还被用于捻转血矛线虫等寄生性线虫疫苗侯选抗原基因的表达和耐药性产生机制方面的研究     

猪瘟病毒及其全长cDNA在猪肾细胞中增殖表达特性的比较研究

以猪瘟C-株弱毒疫苗株、经典强毒石门株和C-株全长cDNA为试验材料，以免疫荧光技术为主；结合ELISA和RT-PCR检测技术，对其在培养细胞中的增殖特性和表达规律进行了比较研究。同时筛选猪瘟病毒及其全长cDNA的敏感细胞，探索其增殖表达规律，以便为猪瘟病毒反向遗传操作提供技术方法和可操作的条件。