对退耕还林工程中种草的探讨

分析种草在退耕还林工程中的重要地位和作用 ,提出退耕种草的草种选择和种植模式 ,并探讨发展草产业的途径和方向     

猪带绦虫TSOL18特异性单克隆抗体的制备及其体外杀虫作用的研究

本研究目的在于制备抗猪带绦虫TSOL18单克隆抗体,并分析单抗对六钩蚴的杀伤作用。采用SephadexG-100纯化在毕赤酵母中表达的猪带绦虫六钩蚴TSOL18蛋白;纯化的蛋白免疫BALB/c小鼠,运用杂交瘤技术建立能分泌抗TSOL18单克隆抗体的细胞株;通过ELISA叠加试验进行mAb的抗原表位分析;采用间接ELISA法测定TSOL18单克隆抗体特异性及腹水效价;采用抗TSOL18单克隆抗体对激活的猪带绦虫六钩蚴进行体外六钩蚴杀伤试验,观察单抗对猪带绦虫六钩蚴活力的影响。结果成功获得12株稳定分泌抗TSOL18单克隆抗体的杂交瘤细胞株,识别2个不同抗原表位,不同抗原表位的2株单克隆抗体腹水效价分别为1×102、1×106。抗体体外六钩蚴杀伤试验结果证明,在有补体的情况下,多抗和单抗作用6 d后,六钩蚴结构模糊,边缘不清晰。表明单抗对六钩蚴有一定的杀伤作用。     

百合组织培养和植株再生的研究进展

综述了百合组织培养和植株再生技术,着重介绍了百合离体快繁、愈伤组织与植株再生、体细胞诱导等方面的研究内容。     

规模养蚕新技术的研究Ⅰ.大棚养蚕温湿度对蚕茧产质量的影响

试验调查了大棚养蚕春、夏大蚕期与蔟中的温湿度变化,以探索有利于大棚养蚕的饲养技术与小气候调节技术.棚内温湿度变化对蚕茧产质量的影响比较分析表明,春期5龄后期和上蔟初期,35～38.8℃的高温对蚕茧产质量有较大影响;夏蚕期棚内温度明显高于春蚕期,最高超过40℃,连续高温持续时间长,昼夜温差大.根据春、夏期不同的气候特点,春期大棚上蔟初期要重点进行降温排湿,夏期重点进行降温.     

双峰驼精清诱导排卵活性成分的DEAE—纤维素柱层析分离及其生物活性

选用DEAE－纤维素离子交换柱对双峰驼精清进行分离，各层析组分用于大鼠垂体组织体外培养，并给母驼肌注有活性的组分，以RIA法测定垂体组织培养液和母驼血浆中LH及FSH的浓度。结果表明，驼精清经DEAE－纤维素柱分离后可得到5个蛋白组分（L1－L5），其中L3在大鼠垂体培养时引起LH释放明显增加（P＜0．05），FSH则无明显变化。给卵巢上无大卵泡发育的母驼肌注有活性的组分L3，可引起血浆中LH明显升高，FSH也无变化。由此表明，L3在体内外试验中均具有引起LH释放的生物活性，它可能是诱导排卵的活性因子或其成分之一。     

发酵麸皮多糖对肉羊肉品质、肌肉氨基酸组成及肌肉抗氧化酶和肌纤维类型相关基因表达的影响

试验旨在研究发酵麸皮多糖(FWBP)对肉羊肉品质、肌肉氨基酸组成及肌肉抗氧化酶和肌纤维类型相关基因表达的影响。选用50只体重为(20.17±3.33)kg的6周龄杜泊×小尾寒羊F1代杂交羔羊,随机分为5组,每组10只。对照组(Ⅰ组)饲喂基础饲粮,试验组(Ⅱ、Ⅲ、Ⅳ、Ⅴ组)分别饲喂在基础饲粮中添加50、100、200、400 mg/kg FWBP的试验饲粮,预试期14 d,正试期56 d。结果表明:1)Ⅱ和Ⅲ组肉羊背最长肌剪切力显著低于Ⅰ组(P<0.05)。2)Ⅱ、Ⅲ和Ⅳ组肉羊背最长肌中蛋氨酸(Met)含量显著高于Ⅰ组(P<0.05);Ⅳ组肉羊背最长肌中的半胱氨酸(Cys)含量显著高于Ⅰ组(P<0.05),而谷氨酸(Glu)和亮氨酸(Leu)含量显著低于Ⅰ组(P<0.05);Ⅴ组肉羊背最长肌中丝氨酸(Ser)含量显著高于Ⅰ组(P<0.05),而缬氨酸(Val)含量显著低于Ⅰ组(P<0.05)。3)各试验组肉羊背最长肌中过氧化氢酶(CAT)的mRNA相对表达量显著高于Ⅰ组(P<0.05),且Ⅲ组肉羊背最长肌中CAT的mRNA相对表达量显著高于Ⅱ、Ⅳ、Ⅴ组(P<0.05)。Ⅲ组肉羊背最长肌中谷胱甘肽过氧化物酶(GSH⁃Px)和超氧化物歧化酶(SOD)的mRNA相对表达量显著高于Ⅰ组(P<0.05)。4)Ⅲ组肉羊背最长肌中肌球蛋白重链(MyHC)Ⅰ和MyHCⅡa的mRNA相对表达量显著高于Ⅰ组(P<0.05),且MyHCⅡx的mRNA相对表达量显著低于Ⅰ组(P<0.05)。Ⅲ、Ⅳ和Ⅴ组肉羊背最长肌中MyHCⅡb的mRNA相对表达量显著高于Ⅰ组(P<0.05)。综上所述,饲粮中添加FWBP可以降低肉羊背最长肌剪切力,改善其氨基酸组成,提高肌肉抗氧化能力,诱导Ⅱx型肌纤维向Ⅰ和Ⅱa型肌纤维的转化,且以添加100 mg/kg效果较佳。     

Effects of main cereal type and feed form on production performance,egg quality and egg sanitary indices of laying hens

ABSTRACT

1. The experiment was conducted to evaluate the effects of cereal types (maize or wheat) and feed forms (pelleted or mash feed) on production performance, egg quality and egg sanitary indices in laying hens.

2. Three hundred and sixty hens (Jinghong No. 1) at 18 weeks of age were randomly assigned to four treatments with six replicates of 15 hens per replicate according to a 2 × 2 factorial design with two cereal types (maize or wheat) and two feed forms (pelleted or mash feed).

3. Compared with the wheat-based diet, the maize-based diet improved (P < 0.05) average egg weight of laying hens. Yolk colour of hens fed with the maize-based diet was higher (P < 0.05) in comparison to those fed the wheat-based diet, while Haugh units were lower (P < 0.05) for the maize-based treatment. Egg mass and average daily feed intake of hens fed the pelleted diet were higher (P < 0.05) than of those fed the mash diet. However, the mash diet improved (P < 0.05) yolk colour compared with the pelleted diet. The percentage of dirty eggs for the wheat-based diet was higher (P < 0.05) than for the maize-based diet. The percentage of dirty eggs was higher (P < 0.05) in birds fed the pelleted diet compared with those fed the mash diet. There were interactions (P < 0.05) between cereal type and feed form, with regard to average egg weight and shell thickness.

4. In conclusion, dietary cereal type affected average egg weight, yolk colour and Haugh units in eggs, while feed form influenced egg mass, average daily feed intake and yolk colour.     

奶牛人工授精的优缺点和技术要点

奶牛人工授精技术加速了奶牛品种改良进程,实现了将优秀种公牛精液跨区域、超时限的利用共享,极大地促进了养牛业的发展.以奶牛人工授精应用现状为出发点,介绍了人工授精技术的优点和不足,最后结合自身工作经验,从发情鉴定、适时配种、精液解冻、精液检测、输精、精液保存和妊娠检测等技术要点进行概述,以期更好地促进奶牛人工授精技术的应...     

播种期对杂交稻种子纯度海南种植鉴定结果的影响

通过比较不同播种时期杂交水稻种子样品在海南种植鉴定的农艺性状表现,发现播始历期、株高、结实率等主要性状均表现出不同程度的变化,且部分组合与其亲本的变化幅度不同,从而影响鉴定的准确性。根据品种特性,确定最佳播种时期,并采用合理的栽培与管理技术,是提高杂交水稻种子纯度种植鉴定结果准确性的关键。     

PULSED WAVE-DOPPLER ULTRASONOGRAPHIC EVALUATION OF THE COMMON CAROTID ARTERY IN THE RESTING HORSE: PHYSIOLOGIC DATA

A pulsed wave-Doppler ultrasonographic evaluation of common carotid arterial blood flow was carried out on 63 healthy Italian Saddlebred horses. Vessel diameter and tracing morphology were evaluated were evaluated and blood flow parameters (systolic, diastolic and mean velocity, acceleration and deceleration of the systolic wave, cartid pulse volume) were calculated and correlated with class variables (sex, age and body weight). On the basis of the presence of an incisure in the ascending branch of the systolic curve, subjects were divided in two groups: one with a bifid systolic curve and the other with a monophasic aspect. Correlations between: 1) diameter of the vessel and body weight and 2) carotid pulse volume and flow velocity (systolic, diastolic and mean) were found. A greater systolic pulse volume was found in male subjects, in subjects with greater body weight and in those which had a monophasic systolic wave.