中华鲟神经内分泌多肽7B2基因克隆及组织特异性表达

以中华鲟(Acipenser sinensis)脑垂体总RNA为模板,采用RT-PCR和RACE方法,获得中华鲟神经内分泌多肽(7B2)基因的3个重叠片段,测序后拼接得到986 bp全长基因序列,其中包括5'端非翻译区(5′-UTR)14 bp、3′端非翻译区(3-′UTR)261 bp和开放阅读框711 bp。翻译编码236个氨基酸。其中前43个氨基酸为7B2的信号肽。经BLAST比对发现中华鲟7B2蛋白的同源性与斑马鱼(Danio rerio)的相似性最高为82%。系统发育分析表明,中华鲟与斑马鱼亲缘关系最近。半定量RT-PCR分析表明:在脑中7B2 mRNA表达量最高,心脏、性腺、胰等组织中表达次之,肠、肾、皮肤等组织中少量表达,肝和鳃几乎不表达。     

Regeneration of adventitious shoots from mature stored cotyledons of Japanese plum (Prunus salicina Lind1)

Adventitious shoot regeneration from mature stored cotyledons of Japanese plum (Prunus salicina Lind1) was achieved in vitro. The influences of the presence and absence of the light, different concentrations of thidiazuron (TDZ) and benzyladenine (BA) in the culture media, TDZ pretreatments and different basal salts on shoot regeneration were evaluated. TDZ was more effective in inducing shoot regeneration from mature stored cotyledons than BA. Dark incubation significantly increased the regeneration frequencies. Quoirin/Lepoivre (QL) basal salts stimulated shoot regeneration more than woody plant (WPM) or B5 salts did. The frequency of adventitious shoot formation varied among the varieties and the regeneration ability appeared to be genotype depended. The frequency of regeneration under the optimum tested conditions for ‘Bruce’, ‘Shiro’, ‘Redheart’, ‘Gladstone’ and ‘Early Golden’ cotyledons were 66.7%, 46.7%, 43.3%, 26.7% and 6.7%, respectively.     

林改宗地数据的网络共享与管理

随着集体林权制度改革的全面展开,宗地信息的网络共享与管理成为一个急需解决的问题。以昆明市宜良县林权制度改革宗地数据为例,基于ASP+HTML+Microsoft SQL Server,采用B/S结构设计实现了宗地数据的网络共享与管理。ASP开发简单而且直观,开发过程易于HTML集成。系统操作简单,维护方便,可让不同用户通过网络获取所需的宗地信息,以及对数据进行管理与维护。     

硼肥在水稻生产上应用效果对比试验

通过在水稻苗期、初花期、齐穗期和灌浆期喷施不同种类的硼肥与空白对照进行对比试验,结果表明:喷施硼肥可增强植株的抗逆性,有利于减少水稻病害的发生,还可改善株型,增强抗倒伏能力;喷施硼肥可以增加水稻的株高、穗长、分蘖数、穗粒数、结实率和千粒重,可以提高水稻产量,各处理的产量均高于对照,增产55.2～93.4 kg/667 m2,增产率12.4%～17.6%,其中产量最高的是速乐硼30 g/667 m2,实际产量达624.1 kg/667 m2,增产率17.6%,增产效果显著。     

Deferoxamine Compensates for Decreases in B Cell Counts and Reduces Mortality in Enterovirus 71-Infected Mice

Enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children under six years of age. No vaccine or antiviral therapy is currently available. In this work, we found that the number of B cells was reduced in enterovirus 71-infected mice. Deferoxamine, a marine microbial natural product, compensated for the decreased levels of B cells caused by enterovirus 71 infection. The neutralizing antibody titer was also improved after deferoxamine treatment. Furthermore, deferoxamine relieved symptoms and reduced mortality and muscle damage caused by enterovirus 71 infection. This work suggested that deferoxamine has the potential for further development as a B cell-immunomodulator against enterovirus 71.     

高校实验室信息管理系统的设计与实现

基于对高校实验室科学化、网络化管理的目的,采用查阅大量相关文献资料,分析国内外研究现状,进行系统设计、实现,并通过实践验证研究效果的技术路线,构建了基于B/S结构模式的高校实验室信息管理系统。通过在河北农业大学基础化学实验室的具体应用,达到了实验室无纸化办公、高效率管理的效果。     

甘蓝型油菜亲本基因型对其与埃塞俄比亚芥的可交配性影响

以10个甘蓝型油菜品种与埃塞俄比亚芥进行了正反杂交。结果表明,甘蓝型油莱与埃塞俄比亚芥的种间可交配性很低,但在反映可交配性强弱的各个性状上,基因型间的差异显著。甘蓝型油菜作母本时,受精频率和结角率都较反交为低,在获得杂种种子和杂种植株上明显优于反交。约2/3的甘蓝型油菜×埃塞俄比亚芥低生活力杂种种子在组织培养条件下能萌发形成植株,反交的萌发率几乎为零。甘蓝型油菜×埃塞俄比亚芥的F_1植株能与甘蓝型油菜回交,与埃塞俄比亚芥回交不亲和。由于种间杂种F_1植株群体中常出现大量的母型植株,且获得的真杂种种子出苗率又很低,因此提出以F_1杂种植株数为基准的可交配指数来衡量种间可交配性高低。     

诱发甘蓝型油菜含油量突变的研究

用~(60)coγ射线照射油菜干种子,诱发含油量突变。结果指出:交变体间含油量的极差达6.99％,世代间的相关性达显著或极显著,亲本含油量对后代含油量的突变影响明显,并初步选育出一批比现有推广品种高5％—7％的高油分品系。     

转抗菌肽B基因和bar基因籼稻植株的再生

 运用基因枪法将含有bar基因和cecropin B基因的质粒pCB1导入籼稻品种特青的幼胚细胞,筛选后得到3株转基因植株。PCR检测和Southern杂交结果表明,外源基因已整合到水稻基因组中。转化当代植株表现出对Basta很强的抗性,同时也增强了对水稻白叶枯病的抗性。     

选育甘蓝型春油菜波里马不育胞质三系杂种的研究

在青海春油菜生态区的田间试验结果表明,长江中游的单,双低半冬性甘蓝型油菜品种资源,绝大部分能正常生长适时成熟,这些品种资源与波里马细胞质雄性不育材料测交和连续回交,大部分半冬性品种的回交后彻底不育,而春性品种的后代出现微粉。半冬性品种转有的不育系或恢复系与春性品种转育的恢复纱或不育系配制的杂种优势明显。今后春油菜区在马不育胞质三系杂种的选育,应用长江中游双低半冬性品种（系）转育的不育系与加拿大和欧