Engineering Calculation Mode Based on Reformative B/S Architecture

Engineering designed mode has entered a new stage because of the development of network. New calculating system based on network appeared. By analyzing the common B/S architecture, this paper brings forward an reformative architecture based on Web services and ASP. NET in order to build the new mode of engineering calculation. It can fully implement the sharing of the resources and provide the engineering designers a wonderful calculating environment, and the working eifficiency of designers can be enhanced.     

基于B/S结构的数字地质图元数据信息系统设计与实现

以国土资源环境与区域经济信息系统信息共享元数据内容标准草案(“NREDIS”)为依据,结合ASP.NET技术,利用C#语言,设计了一个基于B/S(浏览器/服务器)结构的数字地质图元数据信息系统。实现了元数据标准的浏览和元数据的输入、查询及维护4大功能。研究成果为国家空间信息基础设施和数字地球的建设提供了一定的理论基础和技术原型,推动了地理空间信息的网络发布和共享。     

高效数据库应用研究

效率是数据库应用开发过程中经常遇到的问题。为此，对数据库应用中影响效率的相关因素(如大事务、数据库连接等)进行分析，并针对实际电子商务数据库0RACE9I与JAVA应用中出现的问题，提出了解决方案。     

基于BIM技术与B/S架构的大坝安全可视化监测系统

为了实现大坝整体结构、安全监测测点布置及监测成果的可视化展示功能,并且能够及时、准确地掌握大坝运行状态和异常情况,以甘再碾压混凝土重力坝为例,对传统大坝安全监测系统存在的问题进行了深入的分析与解答,并围绕问题提出了可视化的大坝安全监测系统思想,利用Revit、Dynamo、Civil3D等主流BIM软件建立了大坝主体结构及监测点的模型族库,集成了大坝及监测系统的三维可视化BIM模型.借用于BIMFACE平台轻量化技术、PHP脚本语言和MySQL数据库等现代化信息技术,开发出基于B/S架构的甘再大坝安全可视化系统,实现了BIM模型的Web轻量化展示,可通过客户端浏览器在三维模型中直观查看大坝结构、监测仪器信息以及监测点时程曲线变化等内容.通过本系统的应用,大大提高了大坝运行期间安全监测的可视化管理水平,同时对传统的大坝安全监测系统提出了现代化的建立方式与思想.     

干旱区B/S模式水库调度系统设计

利用Visual Studio．NET的优良集成开发环境、采用SQL Server 2000作为B／S计算环境数据库服务器的分布式数据管理系统。针对干旱区水库调度的实际特点，选择了适合当地实际特点的预报、调度模型，考虑到该系统维护、升级的需要，系统采用组件技术，对各子系统进行功能分区，模块化处理。系统具备基本信息接收、存储和查询，洪水预报，水位预报，水库调度，成果管理和数据库管理等主要功能。系统界面简洁、友好，功能适用，方便操作。结合实例展示了系统的在干旱区的应用情况以及在进一步推广应用前景。     

Mechanism of Fuzilizhong decoction alleviating inflammatory damage of rats with non-alcoholic fatty liver disease

AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease （NAFLD） rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups： control group, model group, high dose （20 mg·kg^-1·d^-1）, middle dose （10 mg·kg^-1·d^-1）, low dose （5 mg·kg^-1·d^-1） Fuzilizhong decoction group and Yishanfu （30 mg·kg^-1·d^-1）group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2（IL-2）, IL-6 and tumor necrosis factor-α（TNF-α） were measured by ELISA. The expression of toll like receptor 4（TLR4） and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group（P<0.05）. However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced（P<0.05）.In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway.     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

油研9号氮、钾、硼肥的施用技术

为了探讨油菜良种良法配套栽培技术,以新品种油研9号进行了氮肥的不同施用比例、钾肥的不同施用量、硼肥的不同施用方式的研究。结果表明:在相同施氮量下,以基肥:苗肥:薹肥为3:4:3时产量最高,与5:3:2和6:2:2 的施用比例达显著性差异;与4:3:3的施用比例差异不显著。不同钾肥施用量以底施氯化钾15 kg／667m2产量最高,底施氯化钾均与不施氯化钾达极显著性差异,但底施氯化钾15 kg／667m2比底施氯化钾7．5 kg／667m2增产不显著。不同硼肥的施用方式,以薹施硼肥1 kg／667m2产量最高,薹施(1kg／667m2)、底施(1 kg／667m2)、花喷(100g／667m2)硼肥分别比不施硼肥增产 31．5％、22．8％、5．5％,施用硼肥与不施硼肥均达显著性差异,薹施和底施硼肥增产效果明显,花喷增产效果较差。