Detection of beet yellows virus in sugarbeet plants by enzyme-linked immunosorbent assay (ELISA)

Beet yellows virus can be detected in leaf extracts of infected sugarbeet plants by ELISA. The use of discs was studied and proved to be a valuable and qualitatively reliable method. Leaf material could be stored at 4^o or 22°C for at least six days without affecting the detection of this virus by ELISA. A dramatic decrease in ELISA values was found when leaf extracts were frozen.In an analysis of the distribution of virus over the plant it was found that young leaves present at the moment of infection and those which had still to develop after infection will contain virus. Symptoms produced by systemic virus invasion occur on the oldest leaves containing virus.Samenvatting Het bietevergelingsvirus kan op betrouwbare wijze met de ELISA methode in geïnfecteerde bieteplanten worden aangetoond. Een aanzienlijke vereenvoudiging van de procedure kan worden bereikt met de zogenaamde disc-method, waarbij intacte ponsstukjes in de putjes van de ELISA-plaat worden geïncubeerd. Hierbij komt voldoende virus uit de ponsstukjes voor ELISA vrij. Bladmateriaal kon op verschillende wijzen bewaard worden zonder dat de mogelijkheid om het virus aan te tonen achteruitging. Met bladextracten die ingevroren waren, werden echter slechte resultaten verkregen.In een analyse naar de verdeling van het virus over het loof bleek het virus voor te komen in de geïnoculeerde bladeren, in die bladeren die op het tijdstip van inoculatie minder dan de helft van hun uiteindelijke lengte bereikt hadden en in de bladeren die nog moesten verschijnen. De symptomen ontwikkelden zich op de oudste systemisch geïnfecteerde bladeren.     

The effect of dose and concentration on D-xylose absorption in healthy,immature dogs

Nine combinations of dosages and concentrations of D-xylose were given orally to eight clinically normal, immature dogs. The concentrations and dosages of D-xylose consisted of 5%, 10%, and 20% at 250 mg/kg, 500 mg/kg, and 750 mg/kg. Serum samples were collected at 0, 30, 60, 90, 120, and 180 minutes. Serum xylose was quantitated using the phloroglucinol microassay technique. A peak in serum xylose concentration was seen for each treatment combination at 60 or 90 minutes after dosing. The dosage effect was important in influencing serum xylose values (P < 0.0001). As the test solution dosages increased from 250 mg/kg to 500 mg/kg and 750 mg/kg, serum xylose values (when dosage was analyzed over the length of the entire test) rose linearly (R(2) = 0.98). The treatment combinations of 5% and 20% xylose solutions dosed at 750 mg/kg produced the highest serum xylose values at the 60- and 90-minute peak intervals. The independent effect of concentration was significant (p < 0.001) but was overridden by the stronger dosage effect. Serum xylose concentrations varied little statistically (p > 0.05) when the 5%, 10%, and 20% solutions were compared at a specific dosage.     

不同干燥失水方式对云南温带优良牧草营养品质的影响研究

采用迅速烘干、太阳晒干和缓慢烘干3种处理对云南温带21个牧草品种的鲜草进行干燥处理,分别测定干草的营养成分。结果表明:以干草的粗蛋白含量为指标,干燥方法以迅速烘干为佳,其次是太阳晒干,最差是缓慢烘干;从干草的粗脂肪考虑,其变化无规律,因牧草品种的不同而不同;其余指标变化不大。     

新鲜蔬菜硝酸盐含量测定的改进试粉法

 为适应新鲜蔬菜硝酸盐快速检测的需要, 在现有试粉法的基础上对硝酸盐测定的试粉法进行了改良研究。结果表明, 本研究建立的直接以去离子水浸提蔬菜匀浆, 混合试粉配方为柠檬酸∶一水硫酸锰∶无水对氨基苯磺酸∶N - 1 - 萘乙二胺盐酸盐∶细锌粉= 30∶4∶1.6∶0.8∶1, 其加入量为0.1 g的改进试粉法, 对于溶液中硝酸盐含量在0～20 mg/L范围时, 显色吸光值与硝酸盐含量呈现良好的线性关系, 相关系数达0.9999, 方法回收率在97.7%～104.5%之间, 相对标准偏差2.71%。用本改进试粉法测定11种蔬菜的硝酸盐含量与国标法测定结果的t检验具有一致性。     

改良热硼酸法高效提取苹果果实RNA

苹果果实、尤其是成熟期的果实中多糖和其他次生物质含量高,难以提取RNA。对此,在原热硼酸法的基础上进行了改进,通过添加提取辅助剂并根据辅助剂特性调整提取步骤,高效、稳定地从苹果果实、尤其是后期果实中提取到了高质量的RNA。所得RNA的A260/A230大于2.0,A260/A280为1.8-2.1,并且RNA产量高,其中前期果实RNA产率在13.40μg/g以上,后期果实RNA产率在7.02μg/g以上,完全可以满足RT-PCR、cDNA-AFLP等分子生物学实验的要求。     

用外翻肠囊法研究高钙条件下有机锰在肉仔鸡小肠中的吸收特点

本试验用外翻肠囊法研究高钙对肉仔鸡不同肠段(十二指肠、空肠和回肠)吸收不同形态锰的影响,以比较有机锰和无机锰在高钙条件下吸收特点的差异。采用单因子完全随机设计,培养液中添加的4种锰源分别为:硫酸锰、弱络合强度的蛋氨酸锰(Mn-MetE)、中等络合强度的氨基酸锰(Mn-AAA)和强络合强度的氨基酸锰(Mn-AAB)。为了扣除内源的影响,设置1个零添加锰水平处理。将40只31日龄AA肉公鸡随机分到以上5个组,每组8个重复,每个重复1只鸡,每只鸡的十二指肠、空肠和回肠分别作为相应肠段外翻肠囊的一个重复。结果表明:1)体外培养回肠肠囊对锰的吸收率显著高于十二指肠(P<0.01);2)回肠肠囊对强络合强度的Mn-AAB中锰的吸收率显著高于硫酸锰或中等络合强度的Mn-AAA(P<0.05)。本试验结果表明:在高钙条件下,络合形态的有机锰在体外培养的肉仔鸡肠囊中的吸收率显著高于无机形态的锰;强络合强度有机锰源中的锰能更强地抵抗小肠内的解离,比弱或中等络合强度的有机锰源更有利于锰的吸收。     

法律本科教学方法之我见

随着我国法治的不断健全，对新时期法律本科教育提出了新的要求，这也是对中国法学教育者传统教学方法的一次新挑战。需要我们吸收传统教学方法的优秀经验，探索适合本科阶段教育特色的教学方法，开展案例教学和实践教学，注重教学中的师生互动关系，改革考试内容和考试方式，以使现阶段法学本科教育更能适应现实社会和法律职业的需求。     

陕西线辣椒病毒病病原检测简报

在陕西省线辣椒主产区多点随机取样,采用酶联免疫吸附法对陕西线辣椒病毒病毒原进 行了鉴定。结果表明,陕西线辣椒病毒病毒原有BBWV、PMMV、CMV、ToMV、TMV、PVY和 CVMV。优势毒原是BBWV、PMMV和CMV。     

单对汰选方法在加速抗高效氯氟氰菊酯棉铃虫品系汰选中的应用

通过设计杂合种群单对杂交方式,研究了加速获得抗性品系的方法。以高效氯氟氰菊酯群体汰选后抗性倍数为4.9倍的棉铃虫种群及其同源对照种群为材料,同时设置常规群体汰选方法与单对汰选方法,研究单对汰选方法在加速抗高效氯氟氰菊酯棉铃虫品系汰选中的作用。结果表明,群体汰选两代后抗性倍数由4.9倍提高到7.4倍, 而单对汰选两代后抗性倍数由4.9倍提高到27.3倍。表明在常规群体汰选中穿插几代单对汰选方法可明显加快棉铃虫种群对高效氯氟氰菊酯的抗性汰选进程。