中国沙棘和俄罗斯沙棘在不同土壤水分条件下保护酶系统和丙二醛的变化

在野外不同生境和田间不同干旱条件下对中国沙棘(Hippophae rhamnoides sinensis)和俄罗斯沙棘(Hippophae rhamnoides)的SOD,CAT和MDA进行研究后发现,野外沟底土壤水分含量显著高于坡上土壤水分含量,因此,沟底中国沙棘叶片的SOD,CAT活性高于坡上,MDA含量则为坡上植株高于沟底植株。田间不同干旱胁迫条件下,随着土壤含水量的降低,沙棘叶片中的SOD活性在胁迫初期显著降低,随着干旱胁迫时间的延长持续降低,沙棘叶片SOD活性表现为中国沙棘雄株>俄罗斯沙棘雌株>俄罗斯沙棘雄株>中国沙棘雌株;CAT活性在胁迫初期有所升高,之后开始逐渐下降,种间的差异为中国沙棘高于俄罗斯沙棘,雌、雄株的差异为雌株高于雄株;膜脂过氧化物质MDA的含量随着干旱胁迫程度的增加和胁迫时间的延长持续增加,雌株叶片内MDA含量高于雄株,中国沙棘的MDA含量高于俄罗斯沙棘。     

基于FVCOM渤海浅水分潮的特征分析

基于非结构有限体积法海洋模型FVCOM,对渤海潮波系统进行数值模拟。利用渤海沿岸共19个潮位站的资料对模拟结果进行验证,计算结果均与实测结果吻合良好。基于此进一步研究渤海3个主要浅水分潮M4、MS4和M6的主要特征,并与前人的研究成果进行对比。结果表明M4和MS4分潮传播特征相似,都存在5个无潮点,其中1个为顺时针旋转,而其余四个为逆时针旋转。对于M6分潮,结果显示存在9个无潮点,两个为顺时针,其余七个为逆时针。M4分潮振幅在近岸处可达25cm,约为M2分潮振幅的10％,MS4振幅次之,M6振幅最小,仅为M4分潮振幅的18%左右。三者振幅均为从外海向近岸迅速增大,表明浅水分潮振幅分布特征与海底地形关系密切。     

Kurilosides A1, A2, C1, D,E and F—Triterpene Glycosides from the Far Eastern Sea Cucumber Thyonidium (= Duasmodactyla) kurilensis (Levin): Structures with Unusual Non-Holostane Aglycones and Cytotoxicities

Six new monosulfated triterpene tetra-, penta- and hexaosides, namely, the kurilosides A₁ (1), A₂ (2), C₁ (3), D (4), E (5) and F (6), as well as the known earlier kuriloside A (7), having unusual non-holostane aglycones without lactone, have been isolated from the sea cucumber Thyonidium (= Duasmodactyla) kurilensis (Levin) (Cucumariidae, Dendrochirotida), collected in the Sea of Okhotsk near Onekotan Island from a depth of 100 m. Structures of the glycosides were established by 2D NMR spectroscopy and HR-ESI mass spectrometry. Kurilosides of the groups A and E contain carbohydrate moieties with a rare architecture (a pentasaccharide branched by C(4) Xyl1), differing from each other in the second monosaccharide residue (quinovose or glucose, correspondingly); kurilosides of the group C are characterized by a unique tetrasaccharide branched by a C(4) Xyl1 sugar chain; and kurilosides of the groups D and F are hexaosides differing from each other in the presence of an O-methyl group in the fourth (terminal) sugar unit. All these glycosides contain a sulfate group at C-6 of the glucose residue attached to C-4 Xyl1 and the non-holostane aglycones have a 9(11) double bond and lack γ-lactone. The cytotoxic activities of compounds 1–7 against mouse neuroblastoma Neuro 2a, normal epithelial JB-6 cells and erythrocytes were studied. Kuriloside A₁ (1) was the most active compound in the series, demonstrating strong cytotoxicity against the erythrocytes and JB-6 cells and a moderate effect against Neuro 2a cells.     

基于大型渔业平台的深远海渔业发展现状与思考

基于渔业产业现状,分析国内外在大型渔业平台、深海网箱和养殖工船方面的发展现状,提出“游弋式”养殖工船及深远海渔业的基本概念。受限于投资成本、技术发展水平等客观因素,中国深远海养殖发展速度缓慢,但近年来取得了一定的发展成效。笔者针对现阶段渔业粗放型生产方式的现状,从国家战略、渔业改革和产业需求等角度分析深远海渔业发展的必要性,认为发展以养殖工船为代表的深远海大型渔业平台是实现渔业强国之路的有效途径之一,走出一条符合中国国情的深远海渔业平台的开发之路势在必行。     

Effect of three diets on the gametogenic development and fatty acid profile of Paracentrotus lividus (Lamarck, 1816) gonads

In this study, the effects of three diets were investigated to enhance Paracentrotus lividus production for commercial purposes. P. lividus were fed ad libitum for 80 days with: diet A—fresh Codium tomentosum Stackhouse, 1797; diet B—formulated using a jellified mix of macroalgae and vegetables, including C. tomentosum (20%), Coralina sp. Linnaeus, 1758 (17%), cabbage Brassica oleracea var. capitata Linnaeus, 1753 (30%), carrot Daucus carota Linnaeus, 1753 (30%) and agar (3%) as a gelling agent. Diet C consisted of maize Zea mays Linnaeus, 1753 (56%) and New Zealand spinach Tetragonia tetragonoides (Pallas, 1781) Kuntze, 1891 (44%). Their effects on the gonadal and somatic growths, gonadosomatic index (GI) and gametogenesis were evaluated, as well as on the total lipid content and fatty acid composition of sea urchin's gonads. Diet A provided high values of eicosapentaenoic acid (EPA). Gonads of sea urchins fed with diet A were found mostly in growth and maturation stages of gametogenesis and showed the lowest lipid content. Sea urchins fed with diet B presented their gonads in the reabsorption stage and had the highest values of omega‐3 polyunsaturated fatty acids (PUFAs). Sea urchins fed with diet C were in the early stages of gametogenesis and had the highest values of lipid content, plus omega‐6 PUFAs. Once as an ingredient in a balanced mix with vegetables, C. tomentosum can be a key factor to the development of new promising high‐quality and low‐cost feed for P. lividus roe enhancement.     

Enhancement of gilthead seabream (Sparus aurata) larval growth by dietary vitamin E in relation to two different levels of essential fatty acids

The objective of this study was to determine the effect of dietary vitamin E on gilthead seabream (Sparus aurata) growth and survival, at two different highly unsaturated fatty acids (HUFAs) levels. Eighteen days old gilthead seabream larvae were fed four formulated experimental diets combining two different dietary levels of HUFAs (M: medium 2.5 + 1.5, DHA + EPA, H: high 5 + 2.5 DHA + EPA g per 100 g) with two different levels of vitamin E (M: medium 540 mg kg^?1, H: high 2900 mg kg^?1): MM, MH, HM, HH (HUFA/vitamin E). After 2‐week feeding trial, the average survival rate was 52.6% and there were no significant differences found among treatments. Increase in vitamin E up to high level markedly improved larval growth, particularly when dietary HUFA levels were lower, suggesting a higher protection value when these fatty acids are more limiting. At medium dietary HUFA levels, increase in vitamin E from medium to high level enhanced larval growth performance in terms of total length. Moreover, increase in vit E enhanced HUFAs content in the larval polar lipids denoting the anti‐oxidative effect of vitamin E.     

海冰与风场对布兰斯菲尔德海峡磷虾渔场作业适宜性的影响

全球变暖越来越受到人们的关注,南极海冰和风场作为气候变化的关键因子,显著影响着渔船作业的安全及经济效率.相较于连续泵吸捕捞渔船,我国普遍采用传统渔船开展南极磷虾捕捞作业,该作业易受到外界环境的影响.本实验针对磷虾捕捞作业的适应性,基于1989—2020年美国国家冰雪中心海冰密集度数据及欧洲中期天气预报中心风场数据,获得...     

Sea turtle by‐catch in the Mediterranean

Sea turtle by‐catch data in the Mediterranean were reviewed and analysed with fishing effort. The results indicate over 132 000 captures per year, with probably over 44 000 incidental deaths per year, while many others are killed intentionally. Small vessels using set net, demersal longline or pelagic longline represent most of the Mediterranean fleet and likely cause more incidental or intentional deaths than large vessels typically using bottom trawl or pelagic longline. When interactions, mortality, intentional killing, size (a proxy for reproductive value) and turtle populations are considered, results indicate that Mediterranean green (Chelonia mydas) and loggerhead turtles (Caretta caretta) are more affected (i) by fishing gears such as bottom trawlers, demersal longlines and set nets, (ii) by small‐scale fisheries, and (iii) by fishing in the eastern basin. Although small‐scale fisheries should be the priority target, available measures are easier to implement on the fewer large vessels. Moreover, these measures are few, and they are not implemented yet, while others should still be tested for the Mediterranean fisheries. Thus, measures for reducing captures or mortality through changing gear‐specific characteristics may help, but probably a more holistic conservation strategy aimed to an ecosystem‐based fishery management for a sustainable fishing would be the only solution for the long‐term survival of Mediterranean Sea turtle populations and their habitats. Small‐scale fisheries should manage marine resources, including turtles, in a responsible and sustainable way. Turtles may not only benefit from but can also help this process if their non‐consumptive value is fully recognized.     

Effects of dietary carbohydrate on weight gain and gonad production in small sea urchins,Lytechinus variegatus

In experiment 1, juvenile sea urchins (n = 80, 0.088 ± 0.001 g wet weight and 5.72 ± 0.04 mm diameter) were held individually and fed ad libitum one of three semi‐purified formulated diets (n = 16 individuals treatment^?1). In the diets, protein was held constant (310 g kg^?1 dry, as fed) and carbohydrate level varied (190, 260, or 380 g kg^?1 dry, as fed). Wet weights were measured every 2 weeks. Total wet weight gain was inversely proportional to dietary carbohydrate level and energy content of the respective diet. In experiment 2, sea urchins (5.60 ± 0.48 g wet weight, n = 40) fed 190 g kg^?1 carbohydrate consumed significantly more dry feed than those fed 260 g kg^?1, but not more than those fed 380 g kg^?1 carbohydrate. Based on differential feed intake rates, sea urchins that consumed more feed also consumed higher levels of protein and had the highest weight gain. Consequently, protein content and/or protein: energy ratio may be important in determining feed utilization and growth among sea urchins in this study. The average digestible energy intake was approximately 70 kcal kg^?1 body weight day^?1, suggesting daily caloric intake of juvenile Lytechinus variegatus is lower than in shrimp and fish.     

3种水质调控方式下参池沉积物酶活性的比较研究

2015年10月至2016年9月对自然纳潮、微孔曝气、养水机3种水质调控方式下海参池塘沉积物中淀粉酶、蛋白酶、碱性磷酸酶、脱氢酶的活性进行了跟踪比较研究。研究结果显示,3种水质调控方式下池塘沉积物中的淀粉酶活性年变化为0.126~0.880 mg/g,年均值(0.410±0.180) mg/g,蛋白酶活性年变化为0.024~0.472 mg/g,年均值(0.190±0.103) mg/g,碱性磷酸酶活性年变化为0.068~1.042 mg/g,年均值(0.340±0.196) mg/g,脱氢酶活性年变化为12.092~52.794 mL/g,年均值(26.980±8.295) mL/g。3种水质调控方式下池塘沉积物中蛋白酶、碱性磷酸酶及脱氢酶活性均在自然纳潮池塘中均值最高,变化幅度最大;淀粉酶活性均值则在自然纳潮池塘中最低,养水机池塘最高,这与养水机池塘有机质最低,自然纳潮池塘最高,养水机池塘沉积物的细菌多样性最高,真菌数量最多有关。表明养水机能够快速去除沉积物中氮、磷有机化合物,有利于池塘的正常物质循环。本研究从沉积物酶活性的角度,探讨了养水机的作用效果与其他两种水质调控方式产生差异的机理。