Effect of Ampelopsis grossedentata Extract on Growth Performance，Diarrhea Rate and Anti-oxidation Function in Mice

The effects of Ampelopsis grossedentata extract on growth performance, diarrhea rate and anti-oxidation function in mice were studied in this experiment. 90 mice were randomly divided into five groups: Blank group, experimental groups Ⅰ,Ⅱ,Ⅲ and control group. The mice in blank group were fed with basal diet, the experimental groups were added with 0.02, 0.04, 0.06 mg/(g·BW) of Ampelopsis grossedentata extract and the control group was added with colistin sulfate in basal diets, respectively. On the 26th day of the trial, the mice were administered folium senna and then record their diarrhea condition. The results showed that, the finial weights of mice in experimental and control groups were higher than that in blank group, and the difference between the blank and control groups were significant (P<0.05). Adding Ampelopsis grossedentata extract had a tendency to increase growth performance and liver weight, but the difference was not significant (P>0.05). The diarrhea rates in the control and experimental groups Ⅱ,Ⅲ were significantly lower than the blank and experimental group Ⅰ(P<0.05). Compared with the blank group, the activity of the serum glutathione peroxidase (GSH-Px) was significantly increased in control group (P<0.05), while the increasing trends were not significant in experimental groups (P>0.05). The activity of catalase (CAT) in control group was significantly higher than that in experimental group Ⅱ (P<0.05), and the serum superoxide dismutase (SOD) activities among the experimental groups were not significant (P>0.05). In conclusions, the growth performance and anti-oxidation function in mice could be increased, while the diarrhea rate could be decreased by adding the Ampelopsis grossedentata extract. It suggested that Ampelopsis grossedentata extract was expected to become a substitute for antibiotics in animal feeds.     

显齿蛇葡萄提取物抗常见呼吸道致病菌活性的研究

选用常见呼吸道感染菌作为供试菌,对显齿蛇葡萄不同极性溶剂提取物的抗菌效果进行测定,并对其抗菌成分进行筛选。结果表明,显齿蛇葡萄不同极性溶剂提取物对常见呼吸道致病菌如葡萄球菌、肺炎球菌、甲型溶血性链球菌、乙型溶血性链球菌、大肠杆菌、绿脓杆菌、白色念珠菌等均表现出抗菌效果,且随二氢杨梅素浓度的提高,其抗菌效果增强。     

日粮中添加藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响

为研究日粮中添加藤茶提取物对不同体重阶段猪生长性能、血液生化指标、抗氧化活性的影响,本研究选用平均初始体重为30 kg的三元杂交(杜×长×大)去势公猪90头,随机分成3个处理,分别为对照组(CON组,饲喂基础日粮)、植物精油复合物组(A组,基础日粮中添加0.03%植物精油复合物)和藤茶提取物组(B组,基础日粮中添加0.03%藤茶提取物),根据不同体重阶段(30～50、50～75、75～100 kg及100 kg～出栏)饲喂不同阶段的基础日粮,每次换料前及试验结束时称重采血,分析不同体重阶段添加0.03%藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响。结果表明:与CON组相比,添加0.03%藤茶提取物显著提高了30～75 kg猪的平均日采食量(ADFI)和平均日增重(ADG)(P<0.05);显著降低了30～50 kg猪血清乳酸(LA)含量(P<0.05);显著提高了50～75 kg猪血液胰岛素(INS)浓度(P<0.05),但对血糖(GLU)浓度无显著影响(P>0.05);显著提高了75～100 kg猪GLU浓度(P<0.05),INS浓度有升高趋势(P>0.05),乳酸脱氢酶(LDH)含量显著降低(P<0.05);各体重阶段血清生长激素(GH)含量均有升高趋势(P>0.05)。B组猪血清总超氧化物岐化酶(T-SOD)活力、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活力、总抗氧化能力(TAOC)在各体重阶段与CON组相比均无显著差异(P>0.05)。与A组相比,添加0.03%藤茶提取物显著提高了30～100 kg猪的ADFI(P<0.05),显著降低了30～50 kg猪血清LA含量(P<0.05),且显著提高了50 kg以上体重阶段猪血清GSH-Px活力(P<0.05)。综合试验结果,添加0.03%藤茶提取物可促进30～75 kg猪生长代谢,在一定程度改善50～100 kg猪的血糖代谢,且添加藤茶提取物效果优于添加等量植物精油复合物。     

Effects of Ampelopsis Grossedentata Flavonoids on Serum Biochemical Indexes and Immune Function of Piglets

This experiment was conducted to study the effects of ampelopsis grossedentata flavonoids on serum biochemical indexes and immune function of piglets.24 healthy piglets were selected and divided into 4 groups for 40 days feeding experiment.Control group was fed with basal diet and treatment groups were fed with 0.25%, 0.5% and 1% AGF adding to basal diet, respectively.At the end of feeding experiment, two piglets from each group were selected and blood samples were collected to analyze the physiological and biochemical indexes of serum.The results showed that AGF could significantly improve the content of ALB in serum (P<0.01), and decrease the serum BUN content in piglets, 0.5% and 1% groups had extremely significant difference with control group (P<0.01);TG content in treatment groups were decreased and 0.25% and 0.5% groups had significant difference with control group (P<0.05).AST content in serum of treatment groups significantly reduced comparing with the control group (P<0.05);ALP content of treatment groups were increased and 0.5% group was extremely significantly different from control group (P<0.01);AGF could extremely significantly improve serum IgM content (P<0.01), extremely significantly increase the IgG content of 0.5% and 1% groups (P<0.01), and improve serum complement C3 and C4 contents of 0.25% and 0.5% groups.In conclusion, AGF could enhance the piglet metabolism ability and immune function.     

藤茶HPLC指纹图谱

为了提供藤茶药材质量标准的依据,本文采用HPLC法建立了藤茶药材指纹图谱.色谱条件为迪马C 18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水(0.1％)磷酸,梯度洗脱,流速1.0mL/min,检测波长291 nmm.采用中华人民共和国药典委员会出版的中药色谱指纹图谱相似度评价系统软件(版本:2004A)进...     

藤茶总黄酮对仔猪血清生化指标及免疫功能的影响

试验旨在研究藤茶总黄酮(AGF)对仔猪血清生化指标及免疫功能的影响。选取24头健康的仔猪随机分成4组,每组6头,对照组饲喂基础日粮,试验组分别饲喂添加0.25%、0.5%、1%AGF的基础日粮,试验期为40d。饲养试验结束后,每组抽取2头仔猪血液进行血清生理生化指标测定。结果显示,仔猪日粮中添加AGF能够极显著提高血清白蛋白(ALB)的含量(P<0.01);降低仔猪血清尿素氮(BUN)含量,其中0.5%、1%组与对照组相比差异极显著(P<0.01);各试验组甘油三酯(TG)的含量均有所降低,其中0.25%和1%组与对照组相比差异显著(P<0.05);各试验组仔猪血清中的谷草转氨酶(AST)含量显著低于对照组(P<0.05),碱性磷酸酶(ALP)含量均有所提高,其中0.5%组与对照组相比差异极显著(P<0.01);日粮中添加AGF能够极显著的提高仔猪血清免疫球蛋白M(IgM)的含量(P<0.01),0.5%和1%组血清中免疫球蛋白G(IgG)的含量极显著高于对照组(P<0.01);0.25%和0.5%组一定程度上提高仔猪血清补体C3、C4的含量。综上所述,AGF能够增强仔猪机体代谢能力及免疫功能。     

绿凤藤茶叶片中氨基酸含量及组成分析评价

[目的]评价藤茶营养价值,为藤茶进一步开发和利用提供科学依据。[方法]以藤茶复叶和幼茎为原料,采用氨基酸自动分析仪对样品各种氨基酸的含量进行检测,并对其组成进行比较与分析。[结果]结果表明,藤茶中总氨基酸含量较高,各种人体必需氨基酸种类齐全,且比例均衡,比值系数分(SRC)为77.83。[结论]在藤茶的各种人体必需氨基酸中,第一限制氨基酸为蛋氨酸+胱氨酸。     

不同采收时期及不同部位绿凤藤茶中多糖含量比较与分析

[目的]了解恩施地区绿凤藤茶的最佳采收时期及采摘部位。[方法]采用分光光度法测定不同采收时期及不同部位绿凤藤茶中多糖的含量。[结果]结果表明,不同采摘时间及不同采摘部位的绿凤藤茶中多糖含量具有明显的差别,不同时间采摘的藤茶以5、8、9和10月多糖含量较高,不同采摘部位的藤茶以15~25 d的展叶中多糖的含量较高。[结论]建议在大部分绿凤藤茶叶片生长15~25d时进行采摘。     

藤茶提取物对小鼠生长性能、腹泻率及抗氧化性能的影响

试验旨在研究藤茶提取物对小鼠生长性能、腹泻率和抗氧化性能的影响。将90只小鼠随机分为空白组、对照组和试验Ⅰ、Ⅱ、Ⅲ组,空白组小鼠饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.02、0.04、0.06 mg/g体重的藤茶提取物,对照组在基础日粮中添加0.02 mg/g体重的硫酸抗敌素。在试验第26天给小鼠灌服番泻叶,观察各日粮处理组小鼠腹泻情况。结果显示,各试验组和对照组小鼠试验末体重均高于空白组,且对照组显著高于空白组（P<0.05）;添加藤茶提取物有提高小鼠生长性能和肝脏重的趋势,但与空白组相比差异不显著（P>0.05）;试验Ⅱ、Ⅲ组和对照组小鼠腹泻率显著低于空白组和试验Ⅰ组（P<0.05）;与空白组相比,对照组谷胱甘肽过氧化物酶（GSH-Px）活性显著提高（P<0.05）,试验组也有提高趋势,但均未达显著水平（P>0.05）;对照组的过氧化氢酶（CAT）活性显著高于试验Ⅱ组（P<0.05）,各试验组之间超氧化物歧化酶（SOD）活性无显著差异（P>0.05）。以上结果表明,日粮中添加藤茶提取物能够能提高小鼠的生长性能及抗氧化性能,降低小鼠的腹泻率,可作为抗生素的替代品进一步研发。     

枝条年龄和生长调节剂对藤茶扦插繁殖的影响

探索不同枝条年龄和生长调节剂对藤茶(Ampelopsis grossedentata)扦插的影响,找出最适合扦插的枝条年龄以及生长调节剂种类和浓度。结果表明,藤茶一年生枝条各项生长指标都高于二、三年生枝条,大量生产时应选用一年生枝条作插穗;用20 mg/kg吲哚丁酸处理藤茶一年生枝条,其不定根长度、发芽数、高生长、生物量、成活率表现最优,用40 mg/kg吲哚丁酸处理藤茶一年生枝条,不定根上发出的侧根数最多。从总体上分析,生产中用20 mg/kg吲哚丁酸处理藤茶一年生健壮枝条效果最理想。