Biodiversity of Diploid D-Genome Aegilops Tauschii Coss. in Iran Measured Using Microsatellites

Microsatellite markers were used to analyse the biodiversity of 57 accessions of different subspecies and varieties of wild Aegilops tauschii (2n = 2x = 14; D genome) collected across the major areas where it grows in Iran. Levels of diversity were high, with numbers of alleles averaging 7.3 (ranging up to 12) and polymorphism information contents averaging 0.6591. One accession was notably more similar to two of the D genome in hexaploid wheats (Triticum aestivum) used as outgroups. Within the Ae. tauschii accessions, no markers were characteristic for taxa or geographical origin, suggesting high gene flow between the subspecies and varieties, although some groupings, which could be related to geographical origin, were evident. This survey demonstrates the high diversity present in wild goatgrass in Iran, and indicates that there is value in sampling for useful genes for wheat breeding.     

The Inheritance of Morphological and Biochemical Traits Introgressed into Common Wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) from <Emphasis Type="Italic">Aegilops speltoides</Emphasis> Tausch

The genetic control of morphological characters and gliadin composition was studied in two bread wheat lines with introgressed segments from Aegilops speltoides Tausch. It was found that the transferred trait of leaf hairiness is controlled by one dominant gene, non-allelic to the known gene, Hl1. It was localized in 7B chromosome. Whole plant non-glaucousness is under the control of an inhibitor gene, allelic to the gene W1^I of wheat located on chromosome 2B. This gene was found to be epistatic to the gene controlling spike waxlessness. The introgressed gene for spike glume color was found to be allelic to the Rg1 gene located on 1BS of common wheat, but it was linked with another allele of the gliadin locus Gli-B1.     

Resistance to stripe rust in Triticum turgidum,T. tauschii and their synthetic hexaploids

Resistance to stripe rust (caused by Puccinia striiformis Westend.) of 34 Triticum turgidum L. var.durum, 278 T. tauschii, and 267 synthetic hexaploid wheats (T. turgidum x T. tauschii) was evaluated at the seedling stage in the greenhouse and at the adult-plant stage at two field locations. Mexican pathotype 14E14 was used in all studies. Seedling resistance, expressed as low infection type, was present in all three species. One hundred and twenty-eight (46%) accessions of T. tauschii, 8 (23%) of T. turgidum and 31 (12%) of synthetic hexaploid wheats were highly resistant as seedlings. In the field tests, resistance was evaluated by estimating area under disease progress curve (AUDPC). Synthetic hexaploid wheats showed a wide range of variability for disease responses in both greenhouse and field tests, indicating the presence of a number of genes for resistance. In general, genotypes with seedling resistance were also found to be resistant as adult plants. Genotypes, which were susceptible or intermediate as seedlings but resistant as adult plants, were present in both T. turgidum and the synthetic hexaploids. Resistances from either T. turgidum or T. tauschii or both were identified in the synthetic hexaploids in this study. These new sources of resistance could be incorporated into cultivated hexaploid wheats to increase the existing gene pool of resistance to stripe rust.     

The use of wheat/goatgrass introgression lines for the detection of gene(s) determining resistance to septoria tritici blotch (Mycosphaerella graminicola)

At the IPK Gatersleben a series of 85 bread wheat (T. aestivum)/goatgrass (Aegilops tauschii) introgression lines was developed recently. Based on the knowledge that chromosome 7D of this particular Ae. tauschii is a donor of resistance to septoria tritici blotch (Mycosphaerella graminicola), a sub-set of thirteen chromosome 7D introgression lines was investigated along with the susceptible recipient variety ‘Chinese Spring’ (CS) and the resistant donor line ‘CS (Syn 7D)’. The material was inoculated with two Argentinian isolates of the pathogen (IPO 92067 and IPO 93014) at both the seedlings (two leaf) and adult (tillering) stages at two locations over 2 years (2003, 2004). The resistance was effective against both isolates and at both developmental stages, and the resistance locus maps to the centromeric region of chromosome arm 7DS. On the basis of its relationship with the microsatellite marker Xgwm44, it is likely that the gene involved is Stb5. Stb5 is therefore apparently effective against M. graminicola isolates originating from both Europe and South America.     

Genetic mapping of the Acph1 locus in Aegilops tauschii

The Acph1 locus of Aegilops tauschii encodes a new electrophoretically ‘fast’ acid phosphatase, whose allelic variation could well be involved in intraspecies differentiation. Genetic mapping via microsatellite (simple sequence repeat) analysis revealed that Acph1 is tightly linked with the marker Xgwm157 near the centromere region of chromosome 2.     

Cytoplasmic homology between Aegilops mutica Boiss. and Ae. ovata L.

Summary Triticum aestivum L. em Thell. (2n=42; AABBDD), and T. durum Desf. (2n=28; AABB) genomes were substituted into the cytoplasms of Aegilops mutica Boiss. (2n=14; M_tM_t), Ae. heldreichii Holzm. (2n=14; MM), Ae. uniaristata Vis. (2n=14; M^uM^u), and Ae. ovata L. (2n=28; C^uC^uM^oM^o), to identify the M-genome diploid cytoplasm donor of Ae. ovata. Substitution of the T. durum genome into Ae. uniaristata cytoplasm resulted in a large proportion of shriveled inviable seeds. A few plump viable seeds were obtained all of which produced male-sterile plants having one univalent or telocentric chromosome from Ae. uniaristata. The T. aestivum plants having Ae. uniaristata or Ae. mutica cytoplasms were fertile. However, Ae. mutica was similar to Ae. ovata in the induction of delayed maturity and tall robust growth habit to the T. durum and T. aestivum plants. Cytoplasms of the other C- and M-genome diploids Ae. umbellulata Zhuk. (2n=14; C^uC^u) and, Ae. heldreichii (2n=14; MM) earlier had been shown to differ from that of Ae. ovata. Therefore, Ae. mutica is the most likely cytoplasm and M-genome donor to Ae. ovata.     

Transfer of powdery mildew resistance from Aegilops variabilis into bread wheat

Winter hexaploid wheat (Triticum aestivum L.) was crossed with Aegilops variabilis to transfer resistance to powdery mildew into wheat. Following two backcrosses to wheat and from 5 to 9 generations of selfing, several disomic addition and substitution lines of hexaploid wheat resistant to the mildew pathogen were isolated. A pair of short satellited chromosomes was always observed in the resistant lines. Further evidence utilizing as markers for homoeologous group 1 HMW glutenin subunits and DNA hybridization with probe pGBX 3076 showed that an alien substitution involved this homoeologous group.     

Fertility restoration and nor suppression caused by Aegilops mutica chromosomes in alloplasmic hybrids and lines

By crossing Aegilops mutica with Triticum dicoccum as a bridge species and backcrossing with common wheat as a recurrent pollen parent, male sterile alloplasmic line(s) were produced. In progeny of the crosses, a self fertile plant with 42 chromosomes was selected and named R 20. From this plant several lines that possessed Rf (fertility restoring) genes and/or powdery mildew resistant genes were obtained. Apparently, the system of sterility-fertility of pollen can be applied for hybrid wheat production. In addition, the disease resistance may be used in breeding. The male fertile lines possessed one or more Ae. mutica sat-chromosome(s), which show the ability to suppress the nucleolar organizing regions of chromosomes 1B and 6B of common wheat. The relation between the sat-chromosomes and male fertility restoration is not yet clear. This revised version was published online in July 2006 with corrections to the Cover Date.     

Genetics of two mechanisms of resistance to Meloidogyne naasi (FRANKLIN) in an Aegilops variabilis Eig. accession

Summary Accession No. 1 of Aegilops variabilis has complete resistance to the root knot nematode, Meloidogyne naasi. F2 segregation in a cross between this and two susceptible accessions showed that one dominant gene named Rkn-mn1 prevented development of galls on the roots and consequently of female nematodes. The study of the numbers of females in galls on the F2 plants allowed detection of a recessive gene, Rkn-mnAv, suppressing development of J2 larvae into females. The presence of Rkn-mnAv also resulted in a decrease of the level of galling. Rkn-mn1 has already been introduced into wheat. The interest in transferring also Rkn-mnAv is discussed in relation to extending durability of the nematode resistance.     

An EMS-induced wheat mutant restoring fertility against photoperiod-sensitive cytoplasmic male sterility

Triticum aestivum cv. 'Norin 26' with Aegilops crassa cytoplasm shows photoperiod-sensitive cytoplasmic male sterility (PCMS). This alloplasmic line is almost completely male-sterile under long-day conditions (≥ 15h), but highly male-fertile under short-day conditions (≤ 14.5h). To obtain male—fertile mutants against PCMS, seeds of the alloplasmic line were treated with ethyl methane sulfonate (EMS). The M₃ generation was evaluated for PCMS expression, and one fertility-restoring (FR-mutant) line showing high male fertility under the long-day conditions was selected. Reciprocal F, hybrids between the FR-mutant and the alloplasmic 'Norin 26' showed male sterility under the long-day conditions, and continuous segregation with respect to the degree of fertility restoration occurred in their F₂ generations. These results indicate that multiple recessive mutations with minor effects, induced in the nuclear genome, are involved in the fertility restoration. In fact, no restriction-fragment-length polymorphisms of mitochondrial DNA between the FR-mutant and the alloplasmic 'Norin 26' are found.