稻瘟菌激活蛋白对植物生长及其生理活性的影响

本研究发现,稻瘟菌激活蛋白可明显提高丝瓜、番茄等发芽率,促进种子发芽整齐,同时对促进植物幼苗生长、健壮也有明显的作用。处理水稻后,控制稻瘟菌效果达50.68%;抗旱综合指数也从55提高到92。经激活蛋白处理后,植物的纤维素酶和醇脱氢酶活性增强,过氧化氢和脯氨酸的含量提高,这些酶和活性物质在促进植物生长抗逆方面能发挥重要作用。     

新型激活蛋白对棉花种子发芽及幼苗生长的影响

试验结果表明,用2~3μg/mL的新型激活蛋白浸泡棉种6h,能显著提高种子的发芽势和发芽率,提高幼苗根重、根长及根系活力,促进幼苗茎杆增粗、植株长高,还可以降低组织中MDA的含量,增强幼苗的抗逆性。     

利用重叠延伸PCR技术进行定点突变研究(英文)

[目的]建立一种高效、便捷、经济的DNA定点突变方法。[方法]以重组人组织型纤溶酶原激活剂rPA(Reteplase)基因为模板,采用重叠延伸PCR技术对3个位点进行定点突变,将突变基因片段克隆到克隆载体pEASY-Blunt上,并通过测序验证突变结果。[结果]测序结果表明3个位点的突变结果与预期完全一致,即第10位引入单个碱基A、第137位碱基C突变为G以及第686位碱基G突变为A,通过重叠延伸PCR技术一次引入3个突变碱基,100%的实现目的位点的定点突变。[结论]该研究成功实现目的位点的定点突变,为rPA基因的进一步克隆和功能研究奠定了基础。同时也表明重叠延伸PCR技术是一种高效、便捷、经济的DNA定点突变方法。     

种子活力剂对杂交水稻种子活力影响的初步研究

种子活力剂处理杂交水稻种子,特别是处理低活力的陈种子,不仅能提高杂交水稻种子活力与发芽率、发芽势;而且对提高田间成秧率与秧苗素质都有明显的效果。     

赤霉素加增效剂对珍汕97A生长发育的影响

赤霉素（ＧＡ３）加均效剂对调节珍汕９７Ａ的生育有明显的生物学效应：能加快株高增长速度、促进抽租、提早花期和花时、解除包颈、改善授粉态势、增加柱头外露率和结实率及千粒重、提高制种产量。配加增效刑可使ＧＡ３的用量适应降低，有利于减少生产成本。     

木本泥炭对红黄壤性水田土壤有机质提升和细菌群落组成的影响

通过工程措施开发新垦耕地是增加耕地面积的重要方法,这些新垦耕地的主要问题为土壤有机质(SOM)低下,而农田SOM培育是一个漫长的过程。本研究以新垦红黄壤性水田为研究对象,通过施用腐熟秸秆及与土壤腐殖质结构类似的木本泥炭,明确其快速提升SOM效应。此外,为了促进秸秆养分的快速转化,本研究进一步比较分析了3种商用激发剂的激发秸秆分解潜力,并采用16s高通量测序技术阐明其微生物机制。试验包含6个处理:对照(CK)、施用腐熟秸秆(S)、施用腐熟秸秆+木本泥炭(SP)、以及在SP基础上添加激发剂Ⅰ、Ⅱ、Ⅲ(SPJ1、SPJ2、SPJ3)。结果表明,SP、SPJ1、SPJ2、SPJ3中的土壤有机质(SOM)、易氧化碳(EOC)、可溶性有机碳(DOC)分别比S或CK处理高12.1%～20.6%、29.9%～48.9%、50.8%～75.5%,微生物生物量碳(MBC)则为它们的10.5倍～16.1倍,导致MBC/MBN增加了5.1倍～12.4倍;土壤有效磷(Ava P)和团聚度在SPJ1、SPJ2、SPJ3间类似,并比SP、S、CK处理显著提高44.7%～63.6%、26.4%～43.6%,表明木本泥炭以及本试验所选择的3种激发剂均能显著改善土壤理化性状。细菌多样性和系统发育多样性指数在激发剂施用土壤中均处于较高水平,并分别与DOC和EOC显著正相关。PcoA与Anosim分析发现3种激发剂施用土壤的细菌群落结构类似并与其他处理差异显著,该差异主要与Ava P、DOC、EOC、MBN有关。上述结果表明,通过在新垦水田土壤上施用腐熟秸秆和木本泥炭,可快速提升SOM含量,而在此基础上添加商用激发剂具有激发养分转化功能的微生物的潜力,从而改善土壤理化性质和提升作物产量,并且本研究选择的3种激发剂均有类似效应。     

Regulation of ectopic trypsin and proinflammatory cytokine expression by NF-κB and AP-1 in influenza A virus induced myocarditis

AIM: To investigate the regulatory effects of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) on the expression of ectopic trypsin and proinflammatory cytokines in influenza A virus (IAV)-induced myocarditis. METHODS: Male BALB/c mice of 8 weeks old (n=40) were randomly divided into 4 groups: normal control group (NC), infection control group (IC), NF-κB inhibitor group (NI) and AP-1 inhibitor group (AI). The mice in NC group and IC group were instilled intranasally with 15 μL saline and 40 plaque forming units (PFU) IAV, respectively. The mice in NI group and AI group were infected intranasally with 40 PFU IAV and injected intraperitoneally with 10 mg/kg NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) or 2.5 mg/kg AP-1 inhibitor nordihydroguaiaretic acid (NDGA) once daily. The mice were euthanized at day 9 after instillation, and the hearts were removed for pathological and biochemical analysis. RESULTS: IAV infection induced significant up-regulation of ectopic trypsin, and proinflammatory cytokines interleukin 6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) in the myocardium, and triggered acute myocarditis. PDTC significantly inhibited NF-κB activation and up-regulation of ectopic trypsin and proinflammatory cytokines, and effectively suppressed IAV replication and myocardial inflammatory response (P<0.01). NDGA effectively inhibited AP-1 activity (P<0.01) and mildly suppressed up-regulation of proinflammatory cytokines (P<0.05), but had no effects on the expression of ectopic trypsin, IAV replication and the extent of myocarditis (P>0.05). CONCLUSION: IAV infection induces up-regulation of ectopic trypsin and proinflammatory cytokines in myocardium predominantly by the activation of NF-κB. AP-1 signaling pathway might be only partially involved in the regulation of proinflammatory cytokines.     

不同理化处理对钾长石中钾的促释效果研究

研究了不同活化剂、活化水的理化处理手段对钾长石中钾的促释效果。结果表明:活化水、不同烘干温度、不同活化剂及其用量对钾长石的活化效果均有明显影响;活化水较普通水的促释效果提高12%~21%,NaCl等活化剂与钾长石中的钾发生离子替换作用,促进钾释放,增幅达41%~76%,而活化剂QN和linN不同用量及不同烘干温度条件下,能够大大促进钾释放,增幅达2.8~4.8倍;促释技术为难溶性钾矿物的开发利用提供了一条新的利用途径。     

Inhibitory effects of Zuo-Jin-Wan and its alkaloidal ingredients on activator protein 1, nuclear factor-κB, and cellular transformation in HepG2 cells

Zuo-Jin-Wan (ZJW) has been used to treat hepatocellular carcinoma in Asia. This study was to determine whether ZJW and its components blocked activator protein 1 (AP-1) and nuclear factor-κB (NF-κB) activities as well as tumor promotion in hepatoblastoma HepG2 cells. ZJW and its components, Coptis chinensis and Evodia rutaecarpa, inhibited AP-1 and NF-κB activities, and suppressed anchorage-independent growth of HepG2 cells. The major alkaloidal ingredients, berberine and evodiamine, inhibited AP-1 activities and/or NF-κB activation, and further suppressed hepatocellular transformation. In conclusion, ZJW and its constituents, berberine and evodiamine, suppressed tumor promotion primarily through AP-1 and/or NF-κB pathways in HepG2 cells.