全文获取类型
收费全文 | 26217篇 |
免费 | 1108篇 |
国内免费 | 2143篇 |
专业分类
林业 | 2892篇 |
农学 | 1966篇 |
基础科学 | 3737篇 |
3514篇 | |
综合类 | 12585篇 |
农作物 | 759篇 |
水产渔业 | 804篇 |
畜牧兽医 | 1837篇 |
园艺 | 521篇 |
植物保护 | 853篇 |
出版年
2024年 | 121篇 |
2023年 | 329篇 |
2022年 | 527篇 |
2021年 | 650篇 |
2020年 | 658篇 |
2019年 | 753篇 |
2018年 | 478篇 |
2017年 | 764篇 |
2016年 | 1049篇 |
2015年 | 925篇 |
2014年 | 1352篇 |
2013年 | 1288篇 |
2012年 | 2051篇 |
2011年 | 2202篇 |
2010年 | 1702篇 |
2009年 | 1756篇 |
2008年 | 1738篇 |
2007年 | 2028篇 |
2006年 | 1653篇 |
2005年 | 1417篇 |
2004年 | 1007篇 |
2003年 | 864篇 |
2002年 | 657篇 |
2001年 | 571篇 |
2000年 | 443篇 |
1999年 | 378篇 |
1998年 | 337篇 |
1997年 | 281篇 |
1996年 | 263篇 |
1995年 | 237篇 |
1994年 | 200篇 |
1993年 | 185篇 |
1992年 | 147篇 |
1991年 | 90篇 |
1990年 | 128篇 |
1989年 | 81篇 |
1988年 | 90篇 |
1987年 | 36篇 |
1986年 | 13篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 4篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1956年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
采用面向对象编程技术和可视化的界面设计方法,运用SQL Server 2005为后台数据库并结合Visual Studio 2005,采用C#为程序设计语言,研发了肉羊信息管理与育种分析系统。根据肉羊育种实际需求,设计了用户管理、数据管理、育种分析和系统帮助四大模块,以实现肉羊育种资料的科学化管理,提高肉羊选种选配的准确度和工作效率。 相似文献
62.
应用杆状病毒系统高效表达猪繁殖与呼吸综合征病毒GP5基因 总被引:3,自引:0,他引:3
将猪繁殖与呼吸综合征病毒CH_la株囊膜糖蛋白 (GP5 )基因定向亚克隆到杆状病毒转移载体pBlueBacHisB中 ,与杆状病毒线性DNA(Bac_N_BlueTMDNA)共转染昆虫细胞sf9,经过三轮蚀斑纯化后 ,获得重组杆状病毒rBac_GP5。用该重组病毒感染sf9细胞后 ,应用间接免疫荧光试验、SDS_PAGE和Westernblot检测表明 ,GP5基因在杆状病毒系统中获得了高效表达 ,表达产物因糖基化程度差异 ,表观分子量有 2 2、2 5和 30kDa三种总计约占细胞蛋白总量的 2 6 .4%。 相似文献
63.
A.A. Ressang A.L.J. Gielkens J. Quak Miss Nel Mastenbroek 《The Veterinary quarterly》2013,33(1):31-33
Summary An enzyme linked immunosorbent assay (ELISA) and the agar gel immunodiffusion test with bovine leukosis virus glycoprotein as antigen (AGIDT‐BLV gp) were further used to test 633 bovine sera for antibodies to BL V. Both tests detected the same number of sera positive (149) or negative (464) for antibodies. Nine sera were negative in the ELISA but found to be weakly positive (2 sera) or bending the control line (7) in the AGIDT‐BLV gp. On the other hand 11 sera were scored negative in the AGIDT‐BLV gp but were weakly positive (9 sera), positive (1), and strongly positive (I) in the ELISA. Both tests are used routinely in this Institute as they complement each other, specially if sera with low antibody titers are under investigation. It is concluded that ELISA can fully replace radioimmunoassays in the serodiagnosis of enzootic bovine leukosis. 相似文献
64.
R.H. Drewes 《African Journal of Range and Forage Science》2013,30(4):174-178
A host of grassland management systems have been published and recommended. Whereas earlier approaches to grassland management emphasized the systematic resting aspect, the systems published since 1966 have tended to stress the degree of utilization of the grassland sward. In an attempt to accommodate the periodic dry periods that are so typical of the semi‐arid grasslands of western Transvaal, the Potch system of management was evolved. It aims at the development of a fodder reserve, firstly by delaying the commencement of summer grazing, and secondly, by allowing prolonged periods of absence in the grazing rotation. High utilization grazing within the concept of controlled selective grazing is prescribed. This approach was tested and demonstrated over a period of five years in an experiment comprising 240 beef breeding cows. It appeared that generally, summer grazing only commenced after the third week of November, and that the mean period of occupation amounted to 20 days, with a period of absence of 87 days. A conception rate of 85% of all the cows and heifers mated, was achieved. A stocking rate of 0.53 LSU ha?1 could be maintained in an area where the general grazing capacity is 0.33 LSU ha?1. 相似文献
65.
Evaluation of a new strategy to modulate skeletal development in Thoroughbred performance horses by imposing track-based exercise during growth 总被引:2,自引:0,他引:2
Rogers CW Firth EC McIlwraith CW Barneveld A Goodship AE Kawcak CE Smith RK van Weeren PR 《Equine veterinary journal》2008,40(2):111-118
REASON FOR PERFORMING STUDY: No data exist on the intensity of exercise required or on possible harmful effects of increasing exercise in foals over the natural level when free at pasture. OBJECTIVES: To investigate whether an increase in workload over free pasture exercise in the period from directly after birth to the start of training is tolerated by Thoroughbred (TB) foals without increasing injury rate or producing other undesired side effects. METHODS: Thirty-three TB foals were allocated to one of 2 exercise groups directly after birth. One group (PASTEX) was raised on pasture and the other (CONDEX) kept under identical circumstances, but was additionally subjected to an exercise protocol of gradually increasing intensity. Foals were monitored periodically and scored for the presence of clinical signs related to the musculoskeletal system (joint effusion, pain at flexion, occurrence of physeal swelling), and radiographs taken at the end of the conditioning phase. Also, behavioural studies were performed to detect any changes in behaviour related to the exercise programme. Cortisol levels were measured in both groups, to assess the level of stress. RESULTS: Workload in the CONDEX group was significantly higher than in the PASTEX group (approximately 30%). Conditioning increased the likelihood for joint effusion in the antebrachiocarpal joint, but reduced tarsocrural effusion and physeal swelling at the lateral distal radius, the third metacarpal bone (medial aspect) and lateral and medial aspects of the third metatarsal bone. CONCLUSIONS: The 30% increase in workload did not affect the animals' welfare, effects of conditioning exercise on clinical musculoskeletal health were few and there were no adverse effects. POTENTIAL RELEVANCE: This study supports the feasibility of imposing early conditioning exercise in horses and is a benchmark for its effects on the development of equine musculoskeletal tissues. 相似文献
66.
本研究通过对陇西南塬区农业生产系统的结构分析,确认该系统的特征为产业结构不合理(以种植业为主),种植业以粮食生产为主,复种指数低(111%);养殖业中草食家畜比例低,饲养技术落后,良种率低;林果业效益低下;人口密度高,劳动力过剩,生产能源短缺等。通过系统分析提出了调控方针,该系统整体效益提高的关键是发展以草食家畜为核心的养殖业,种植业应及时调整结构,提高经济作物和饲料作物的比例,提高复种指数,林果 相似文献
67.
68.
紫花苜蓿品种在金沙江干热河谷地区的生产性能 总被引:1,自引:1,他引:1
基于灰色系统理论,对金沙江干热河谷地区引种的14个紫花苜蓿Medicago sativa品种的鲜草产量、干草产量、株高、分蘖数、茎叶比、蛋白质含量主要生产性能指标进行灰色关联度分析,根据权重比较得出各项生产性能指标的权重顺序为:蛋白质含量>鲜草产量>茎叶比>株高>干草产量>分蘖数;依据各项指标权重构建了紫花苜蓿综合评价模型,并应用综合评价模型对各品种生产性能进行综合评价,结果表明:猎人河(74-55)在综合评价模型中评价值最高,综合生产性能最好,在金沙江干热河谷地区特殊的生态条件下,是最适宜推广种植的优良品种。 相似文献
69.
肉种鸡鸡舍夏季通风降温试验 总被引:1,自引:0,他引:1
在两栋长145m,跨度5.4m的双坡式有窗封闭舍内,采用三层笼养方式分别饲养700只235日龄的艾维菌肉种鸡,将其中采用轴流式风机加湿帘纵向通风系统的一栋鸡舍作为试验组,而将另一栋采用窗户自然通风的鸡舍作为对照组,于夏季进行为期15d的试验,结果表明试验组与对照组的舍内温度在1d内明显不同,且舍外温度越高,试验组的舍内降温效果越好,相应地,试验组也减轻了肉种鸡的夏季热应激。 相似文献
70.
本研究旨在探明鸭源新城疫病毒(NDV)通过人工感染后在种鸭生殖系统中分布。应用本实验室分离的一株经鸭胚传递的NDV SDFCH株,对42只无NDV感染的28周龄樱桃谷种鸭进行人工感染试验,试验分母鸭接种强毒组,公鸭接种强毒组和对照组。强毒接种4 d后,每隔2 d每组各迫杀2只,采集母鸭卵巢、卵泡、输卵管组织,公鸭采集睾丸、输精管进行病毒分离鉴定,并利用RT-PCR方法进行病毒F基因的扩增与同源性分析。同时,以NDV F蛋白的单克隆抗体(MAb)为一抗,建立间接免疫荧光检测方法检测输卵管组织中NDV及病毒在输卵管的分布情况。结果表明采用RT-PCR方法能够在种鸭生殖系统各个组织中检测并重新分离到NDV;输卵管子宫部和蛋白分泌部纤毛柱状上皮的纤毛细胞和分泌细胞内有阳性荧光信号存在。结果表明人工感染的种鸭卵巢、卵泡、睾丸、输精管以及输卵管子宫部和蛋白分泌部纤毛柱状上皮的纤毛细胞和分泌细胞中均有NDV分布,病毒能否随蛋白分泌、精液的排泄进入种蛋传递给雏鸭还有待于进一步研究。 相似文献