广西相思树病害的调查与防治

对广西6种外来相思(马占相思、直干相思、纹荚相思、厚荚相思、杂交相思和黑木相思)的病害进行调查,发现主要病害有4种,次要病害3种,并提出防治方法。相思炭疽病病原为胶孢炭疽菌(Col-letotrichum gloeosporioides),在马占相思、直干相思、纹荚相思、厚荚相思和杂交相思的叶状柄上只产生病原菌的无性阶段,而在厚荚相思叶状柄上可同时出现无性阶段和有性阶段;相思白粉病病原为白粉菌(Oidi-umsp.),为害马占相思、厚荚相思和杂交相思的叶状柄和嫩枝,其中杂交相思白粉病发生普遍而严重,发病期多在3~5月;黑木相思叶状柄尖枯病病原为异色拟盘多毛孢(Pestalotiopsis versicolor),一年四季均可发病;马占相思心腐病,主要发生在树干基部,造成木材的大量损失,病原尚未得到确定。     

Nodulation and estimation of symbiotic nitrogen fixation by herbaceous and shrub legumes in Guinea savanna in Nigeria

Twelve herbaceous and shrub legume species were grown in pot and field experiments in five sites representing three agroecological zones in moist savanna in Nigeria. The objectives were to: (1) assess natural nodulation of the legumes and characterize their indigenous rhizobia, (2) determine their need for rhizobia inoculation and (3) estimate the amount of N₂ fixed by each of these legumes. At 4 weeks after planting (WAP), Crotolaria verrucosa was not nodulated at any of the sites while Centrosema pascuorum had the highest number of nodules in all sites. At 8 WAP, all legumes were nodulated, with Mucuna pruriens having the least number of nodules and Stylosanthes hamata the highest. The number of nodules, however, was inversely correlated to the mass of nodules. Significant differences in nodulation of the legume species grown in the field also occurred between and within sites. Mucuna pruriens and Lablab purpureus produced more shoot and nodule biomass than the other legumes in all sites. Growth of most of these legumes responded to fertilizer application, except for C. verrucosa and Aeschynomene histrix. Except for C. verrucosa, average proportion of N₂ fixed was about 80% and this was reduced by about 20% with N fertilizer application. The majority of rhizobia isolates (60%) were slow growing, belonging to the Bradyrhizobia spp. group. Selected rhizobia isolates evaluated on Cajanus cajan, C. pascuorum, M. pruriens and Psophocarpus palustris varied from ineffective to highly effective in Leonard jar conditions. However, only growth of M. pruriens responded to inoculation in potted soils, whereas it was lower than that obtained with N fertilizer application. This indicated the need to screen more rhizobia in order to improve N₂ fixation and growth of legume species such as M. pruriens when it is introduced in soils deficient in N.     

Rhizobium sp. exopolysaccharide production,nodulation, and acetylene reduction activity (N2-fixation) in pigeon pea (Cajanus cajan L.)

Summary There was no correlation between the quantity of exopolysaccharide produced and acetylene reduction activity by Rhizobium spp. or by Bradyrhizobium spp. (Cajanus). The exopolysaccharide-defective mutants of Rhizobium sp. strain P 116 either failed to nodulate or showed a decrease in effectiveness. The deficiency in exopolysaccharide production was corrected by the addition of purified exopolysaccharide from the parent strain, or from Bradyrhizobium sp. strain P 149 or S24 isolated from pigeonpea (Cajanus cajan) and mungbean (Vigna radiata), respectively. However, the nodules so formed were not fully effective compared to those formed by the parent strain.     

Effects of herbicides on three vesicular-arbuscular fungi associated with winter wheat (Riticum aestivum L.)

Summary We studied the effects of field application rates of four herbicides (Avenge, Ceridor, Dicurane, and Harrier) on spore germination and infection of wheat roots by three species of Glomus grown under conditions of low P availability. Low concentrations of Ceridor (bifenox, mecoprop) and Harrier (mecoprop, ioxynil, clopyralid) inhibited spore germination while higher concentrations were stimulatory. Avenge (difenzoquat methyl sulphate) prevented spore germination completely, while Dicurane (chlortoluron) had no effect. The herbicide applications had no significant effect on the infection rates of any of the three fungi except Harrier on G. geosporum. The herbicide treatments did, however, affect plant growth and ear yields. Ceridor and Harrier increased ear yields, while Dicurane showed marked phytotoxic effects.     

Reduction of bacterial growth by a vesicular-arbuscular mycorrhizal fungus in the rhizosphere of cucumber (Cucumis sativus L.)

Summary Cucumber was grown in a partially sterilized sand-soil mixture with the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum or left uninoculated. Fresh soil extract was places in polyvinyl chloride tubes without propagules of mycorrhizal fungi. Root tips and root segments with adhering soil, bulk soil, and soil from unplanted tubes were sampled after 4 weeks. Samples were labelled with [³H]-thymidine and bacteria in different size classes were measured after staining by acridine orange. The presence of VAM decreased the rate of bacterial DNA synthesis, decreased the bacterial biomass, and changed the spatial pattern of bacterial growth compared to non-mycorrhizal cucumbers. The [³H]-thymidine incorporation was significantly higher on root tips in the top of tubes, and on root segments and bulk soil in the center of tubes on non-mycorrhizal plants compared to mycorrhizal plants. At the bottom of the tubes, the [³H]-thymidine incorporation was significantly higher on root tips of mycorrhizal plants. Correspondingly, the bacterial biovolumes of rods with dimension 0.28–0.40×1.1–1.6 m, from the bulk soil in the center of tubes and from root segments in the center and top of tubes, and of cocci with a diameter of 0.55–0.78 m in the bulk soil in the center of tubes, were significantly reduced by VAM fungi. The extremely high bacterial biomass (1–7 mg C g^-1 dry weight soil) was significant reduced by mycorrhizal colonization on root segments and in bulk soil. The incorporation of [³H]-thymidine was around one order of magnitude lower compared to other rhizosphere measurements, probably because pseudomonads that did not incorporate [³H]-thymidine dominated the bacterial population. The VAM probably decreased the amount of plant root-derived organic matter available for bacterial growth, and increased bacterial spatial variability by competition. Thus VAM plants seem to be better adapted to compete with the saprophytic soil microflora for common nutrients, e.g., N and P, compared to non-mycorrhizal plants.     

Ecology of soil microflora and mycorrhizal symbionts in degraded forests at two altitudes

Summary Microbial populations were estimated in four different forest stands at different regenerational stages, two each at higher and lower altitudes. The fungal and bacterial populations showed marked seasonal variations at both altitudes. Quantitatively, the bacterial population was higher than the fungal population. Although 25 fungal species were isolated at the lower altitude, only 15 were obtained at the higher altitude. Penicillium chrysogenum and Trichoderma viride were dominant at the lower and higher altitudes, respectively. In the more degraded forest stand at the lower altitude both the fungal and the bacterial population showed a significant positive correlation with organic C (r=0.658 and 0.735, respectively), whereas in the less degraded forest stand there was a significant correlation only between the fungal population and organic C (r=0.835). At the higher altitude, however, a highly significant correlation (P<0.05) was observed between the fungal population, soil moisture and organic C in both the forest stands. Disturbance to the soil and vegetation adversely affected the microbial population, and also affected endogonaceous spores. At the lower altitude, plants in the more degraded forest stand were more mycotrophic compared to those in the less degraded stand. The level of mycorrhizal infection showed a highly positive correlation with soil moisture, organic C, total N, and available P. The spore population, however, was correlated negatively with these parameters. Three different endogonaceous genera, Glomus, Gigaspora, and Acaulospora, were identified during the course of investigation. Glomus, however, was dominant.     

The severity of field pea diseases depending on sowing rate and variety

ABSTRACT

Six varieties of field pea (Pisum sativum L.) were sown at two sowing rates over two years to assess the impact on disease occurrence in a Baltic climate. The varieties used were Bruno (Latvia), Capella (Sweden), Clara (Sweden), Kirke (Estonia), Onward (Greece), and Vitra (Latvia). The two sowing rates were 120 seeds per m² and 144 seeds per m². The increase in sowing rate by 20% did not influence field pea diseases. Variety influenced the incidence of disease in field pea. The varieties most damaged by pod spot (Ascochyta blight caused by Ascochyta spp.) were Vitra and Onward and least damaged were Bruno and Clara. The variety most damaged by downy mildew (caused by Peronospora viciae) was Vitra and least damaged was Bruno. The variety most damaged by white mould (or Sclerotinia rot caused by Sclerotinia sclerotiorum (Lib.) de Bary) was Onward. The varieties that had the most disease damage were Vitra and Onward, while least susceptible were Clara and Bruno. Therefore, Clara and Bruno are recommended for field pea production in a Baltic climate.     

越南和平水电站库区不同农林复合模式的环境效益比较

为了找出最适宜于越南中部山区、预期综合环境效益最佳的、更利于生态平衡的农林复合模式,该研究以越南和平水电站库区4种主要农林复合模式：马占相思树（Acacia mangium）-玉米（Zea mays）、马占相思树-木薯（Manihot esculenta crantz）、赤桉树（Eucalyptus camandulensis）-旱稻（Oraza sativa）和马占相思树-木薯-玉米为研究对象,采用盖度、土壤理化性质、土壤蓄水能力、水土保持能力等指标进行环境效益的比较,最后得出的结论为：在4种农林复合模式中,马占相思树-木薯-玉米复合模式具有最高的水土保持效益和综合环境效益;赤桉树-旱稻复合模式的水土保持效益和综合环境效益均最低;实践中应该增加前者的种植面积,限制和减小后者的种植面积。在马占相思树-木薯-玉米复合模式的基础上加以改进,提出了预期综合环境效益更好的马占相思树-木薯-玉米-肉桂（Cinnamomum cassia）复合模式,为农林复合系统在和平水电站库区的应用提供更好的选择,同时也为世界上有类似气候环境条件的地区农林复合模式的采用提供参考。     

Evaluation of nitrogen fixation by different strains of theAzolla-Anabaena symbiosis in the presence of a high level of ammonium

Summary The proportion of N derived from N₂ fixation for 99 strains ofAzolla spp. (comprising all known species) in the presence of ammonium (40 mg/1) was assessed using a¹⁵N-dilution technique. The percentage of N derived from air varied from 29.5% to 79.9%. Although the N concentration ofAzolla spp. was not correlated with fertilizer N, it correlated fairly well with N₂ fixation. Regression analysis suggests that the N yield ofAzolla spp. is more dependent on N₂ fixation than on ammonium assimilation. The high correlation between N yield and isotopically determined, fixed N₂ indicates that the N yield could be used as a parameter in the selection ofAzolla spp. strains that are capable of maintaining high N₂ fixation in the presence of a high level of ammonium.     

辐照木霉菌株的生物学效应研究

本文研究了6 0 Coγ射线辐照木霉 (Trichodermaspp)菌株的诱变效果。结果表明 :在γ射线的作用下 ,木霉分生孢子萌发率显著降低 ,孢子萌发时间加长 ,部分萌发孢子菌丝生长畸形 ;随着照射剂量的增加 ,木霉孢子存活率急剧下降 ,剂量效应曲线符合一次击中模型S =e-λD;诱变效应方程为 :Y =-0 940e1 2 9x,r=0 953 3 (r0 0 1=0 765)。依各菌株对生长速度、产孢量、开始产孢时间、菌落颜色等变异情况 ,初筛得到变异菌株 1 3 0株。