Renal amyloidosis in cattle with inflammatory diseases

BACKGROUND: The association of inflammatory diseases such as traumatic reticuloperitonitis (TRP), mastitis, metritis, and pododermatitis with renal amyloidosis in cattle is poorly described. HYPOTHESIS: Serum amyloid A (SAA) levels are elevated during inflammatory diseases, and renal amyloidosis is formed as a complication. ANIMALS: This study was conducted with 82 crossbred cattle with mastitis (n = 18 cows), metritis (n = 11 cows), TRP (n = 30 cows), and pododermatitis (n = 23 : 15 cows and 8 beef cattle). Ten clinically healthy cows served as controls. Methods: Hematological, urinary, and blood parameters, including SAA, were measured by an automated procedure provided with trade kits. Determination of amyloidal structures was made by histopathological examination of renal biopsy specimens. RESULTS: At the end of this trial, amyloidosis was detected in 5 cows displaying typical nephrotic syndrome, with hypoproteinemia and proteinuria in combination with polyuria and weight loss. Furthermore, it was observed that cows with renal amyloidosis had significantly higher (P < .01) total leukocyte counts, serum and urine enzyme activities, and urea and creatinine concentrations, with lower serum total protein concentrations, when compared with animals without renal amyloidosis. CONCLUSIONS AND CLINICAL IMPORTANCE: The incidence of AA amyloidosis in cattle in this study suggests that cattle with mastitis, metritis, and pododermatitis have a high prevalence of systemic amyloidosis in response to inflammation.     

O型口蹄疫病毒P1-2A3C基因在家蚕杆状病毒表达系统中的表达

口蹄疫是一种严重危害畜牧业生产的烈性传染病。为了促进O型口蹄疫病毒(FMDV)基因工程活载体疫苗的研制,选取O型FMDV编码序列中的衣壳蛋白前体P1-2A基因和蛋白酶3C基因,插入家蚕杆状病毒转移载体pVL1393中,构建重组载体pVL-P1-2A3C,并与线性化病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞,获得重组病毒Bm-P1-2A3C。将重组病毒感染家蚕5龄幼虫,以双抗体夹心ELISA法和间接血凝方法检测血淋巴中的表达产物:目的蛋白在感染病毒后120 h的蚕血淋巴中表达量最高,抗原表达呈阳性的最大稀释倍数为1∶128。结果显示O型FMDV的P1-2A3C基因已在家蚕体内获得表达。     

蓝狐与银狐褪黑激素受体1A基因的克隆及序列分析

根据其它物种褪黑激素受体（melatonin receptor, MTNR）1A基因核酸序列的同源性设计引物,PCR扩增蓝狐和银狐MTNR1A基因的546 bp DNA序列,GenBank登录号分别为EU170442和EU170443。蓝狐和银狐MTNR1A基因DNA序列同源性为99.1%,编码的氨基酸同源性为99.4%,与人、鼠及绵羊MTNR1A基因同源性为84%~86%,与鸡MTNR1A基因同源性为74%。     

Characterization of an intravenous lipopolysaccharide inflammation model in calves with respect to the acute-phase response

Our objective was to develop a lipopolysaccharide (LPS) inflammation model in calves to evaluate the acute-phase response with respect to the release of pro-inflammatory cytokines and acute-phase proteins, fever development and sickness behaviour. Fourteen 4-week-old male Holstein Friesian calves were included and randomly assigned to a negative control group (n = 3) and an LPS-challenged group (n = 11). The latter received an intravenous bolus injection of 0.5 μg of LPS/kg body weight. Blood collection and clinical scoring were performed at 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, 8, 12, 18, 24, 28, 32, 48, 54 and 72 h post LPS administration (p.a.). In the LPS group, the following clinical signs were observed successively: tachypnoea (on average 18 min p.a.), decubitus (29 min p.a.), general depression (1.75 h p.a.), fever (5 h p.a.) and tachycardia (5 h p.a.). Subsequent to the recovery from respiratory distress, general depression was prominent, which deteriorated when fever increased. One animal did not survive LPS administration, whereas the other animals recovered on average within 6.1 h p.a. Moreover, the challenge significantly increased plasma concentrations of tumour necrosis factor-α, interleukin 6, serum amyloid A and haptoglobin, with peaking levels at 1, 3.5, 24 and 18 h p.a., respectively. The present LPS model was practical and reproducible, caused obvious clinical signs related to endotoxemia and a marked change in the studied inflammatory mediators, making it a suitable model to study the immunomodulatory properties of drugs in future research.     

滨藜灌木在优质羊肉形成过程中的作用

主要分析由耐盐植物滨藜灌木加工而成的饲料对羊肉品质的影响。滨藜可以提高胴体中瘦肉的比例．同时可增加肌肉中VE的含量．保持肉色稳定。概述了滨藜在羊肉生产中的最佳利用方式,通过综合考虑放牧条件和动物消化能力两方面因素,改变肉质组成和提高VE含量。滨藜的利用难点是能量值较低、增重效果差,单独饲喂满足不了肉羊生产需要,所以必须在饲料中补充高能牧草或精料。强调了耐盐滨藜灌木在羊肉生产中的作用,由于山羊具有采食滨藜灌木枝芽的特性,所以其增重较明显．同时又可提高瘦肉率和VE含量。     

维生素A调节动物一氧化氮的生成及其相关机制

动物机体由于旺盛的新陈代谢或在受到外界有害因素刺激的情况下会产生大量一氧化氮(NO),进而损伤细胞中的蛋白质、类脂膜和DNA,引发细胞炎症并阻断细胞信号通路,使机体产生氧化应激。维生素A可以有效地调控NO的生成,提高机体抗氧化水平并清除自由基,预防细胞炎症及氧化应激的发生。本文主要综述了维生素A对动物NO生成的调节作用及其相关机理的研究进展,对今后深入研究其调节机理、科学补充维生素A及提高机体的抗氧化功能具有一定参考价值。     

甘草查尔酮A对副猪嗜血杆菌的体内外抑制作用

探讨甘草查尔酮A对副猪嗜血杆菌体内外生长的影响。将副猪嗜血杆菌于分别含10、20、50、100、200μg/mL甘草查尔酮A的TSA液体培养基中培养,采用分光光度法分析副猪嗜血杆菌生长和生物被膜形成的变化;以1、10、100mg/kg甘草查尔酮A经口灌胃小鼠,分别在3d、5d和7d称重,并计算脏器指数,Hiseq高通量测序技术分析肠道菌群等生长性能,Bliss法测定副猪嗜血杆菌对小鼠半数致死量(LD50);检测甘草查尔酮A对小鼠清除0.01×LD50副猪嗜血杆菌感染和抵抗100×LD50副猪嗜血杆菌感染的影响。结果表明,各浓度甘草查尔酮A对副猪嗜血杆菌的生长速率和生物被膜形成的影响差异均无显著差异。所有剂量组小鼠给予甘草查尔酮A处理后,一般临床症状、体重和脏器指数无统计学意义。灌喂甘草查尔酮A有增加小鼠肠道菌群丰富度的趋势,但降低拟杆菌门丰度而增加厚壁菌门丰度。小鼠清除0.01×LD50副猪嗜血杆菌感染的能力呈剂量依赖性,而100×LD50的副猪嗜血杆菌感染小鼠后随着甘草查尔酮A浓度增加可呈剂量依赖延迟小鼠死亡时间。表明甘草查尔酮A对副猪嗜血杆菌体外生长无显著的抑制作用,同时对小鼠生长性能亦无明显影响,但可增加肠道菌群丰富度而提高小鼠抵抗副猪嗜血杆菌感染的能力。     

某牛场奶牛A型多杀性巴氏杆菌的分离鉴定

湖北某奶牛场牛群爆发体温升高、呼吸困难、流涎及颈胸皮下气肿等症状的疾病,部分发病牛衰竭死亡,为确诊牛场牛群发病原因并提出防控方案,本试验采集死亡牛的心脏、肝脏、肺脏及气管组织进行病原菌的分离纯化及PCR鉴定,并对鉴定的病原菌进行生化特性鉴定、致病性试验和药物敏感性分析;同时提取患牛血清RNA,开展牛流行热病毒的RT-PCR鉴定。结果显示,病料在类胸膜肺炎固体培养基上不生长,生化特性鉴定显示能形成靛基质、不发酵肝糖和肌醇;牛流行热病毒RT-PCR扩增阴性;所分离的病原菌经16S rRNA及牛A型多杀性巴氏杆菌特异性引物PCR扩增阳性;致病性试验显示该病原可致死小鼠,且能从死亡小鼠体内分离到感染菌;药敏试验结果显示该病菌对头孢哌酮、左氧氟沙星敏感,其他20种临床常见药物表现耐药。综上所述,该牛场患病牛确诊为牛A型多杀性巴氏杆菌感染,建议根据药敏试验结果选择敏感药物,对患病牛进行隔离治疗,疑似患病牛隔离观察,同时加强通风,及时清理污物并消毒,改善饲养管理,避免拥挤、寒冷及长途运输等应激因素。     

海藻多糖对肉鸡生长性能影响的研究

在肉鸡饲粮中添加1.5 %海藻多糖 ,对山东地方特色保种白羽鸡进行饲养试验 ,用聚丙烯酰胺凝胶电泳(SDS)PAGE技术分析血液中脂酶(EST)、乳酸脱氢酶(LDH)、蛋白质的多态。结果表明 ,处理组90Ku蛋白亚基组分表达程度显著增强。EST同工酶快区Es -1位点 ,由多等位基因控制 ,酶带着色程度显著减弱 ,其活性下降42.7 %。LDH同工酶出现3条酶谱带 ,酶带着色的程度明显增强 ,其活性增加3倍。此外 ,添加组的饲料利用率、增重及生长性能明显提高 ,提示海藻多糖直接或间接调控基因表达水平 ,与生长性状呈正相关。     

过表达NR4A1促进山羊皮下脂肪细胞分化

旨在克隆山羊N R4A1基因的CDS区序列,明确其组织和细胞表达模式,以及探究过表达N R4A1基因对山羊皮下脂肪细胞分化的影响.本试验利用双酶切法构建山羊过表达载体pcDNA3.1-NR4A1.以1周岁简州大耳羊(n=5)为试验动物.利用RT-PCR方法和实时荧光定量PCR(real-time quantitativ...