6-硫-6-烷硫基-12H-氯溴代双苯并[d,g][1,3,2]-二氧磷杂八环的合成

通过双 [2 -羟基 - 3-溴 (氯 ) - 5-氯 (溴 )代苯基 ]甲烷 4 A(4 B)与 P4 S10在三乙胺存在下关环 ,得到中间体 6 -硫 -巯基 - 4 ,8-二溴 (氯 ) - 2 ,10 -二氯 (溴 ) - 12 H -双苯并 [ d,g ][1,3,2 ]-二氧磷杂八环三乙胺盐 5A(5B)。后者与卤代物反应得到了一系列相应的酯 6。测试并讨论了合成的 6的 1H、31P、13 C NMR、MS及抑菌活性。     

Tet-on系统诱导猿猴病毒40T在CHO细胞中的表达

四环素诱导表达系统（Tet-off／Tet-on系统）是比较成熟的真核生物基因诱导表达系统之一,具有高效、无毒、严密开／关功能的特点。猿猴病毒40T（SV40T）是一种病毒癌蛋白,其与肿瘤抑制蛋白p53和Rb结合,并使之失活,从而消除它们抑制细胞生长的功能,使细胞分裂加速,形成肿瘤。利用Tet-on系统首先稳定筛选获得了表达Tet-on系统调节元件rtTA的阳性细胞CHO-pTet-on,再通过稳定筛选又成功得到导入其反应元件的双阳性细胞CHO-pTet-on-pTRE2-SV40T-Hyg,经强力霉素诱导表达了目的基因SV40T,建立了Tet-on基因诱导表达系统的细胞诱导表达研究平台。     

拟南芥At4g05060基因的酵母双杂交诱饵表达载体的构建、表达及自激活检测

为研究At4g05060基因功能,采用PCR扩增拟南芥VAMP(vesicle-associated membrane protein)家族基因At4g05060的ORF序列,定向克隆至酵母双杂交诱饵表达载体pGBKT7,经酶切鉴定并测序后将其转化至酵母Y187细胞;然后采用Western blotting技术分析At4g05060基因在酵母中的表达水平,通过缺陷型培养基培养进行自激活检测。结果表明,成功构建酵母诱饵表达载体pGBKT7-At4g05060,并在酵母细胞正确表达,表达产物对报告基因无自激活作用。     

40％噻虫嗪·溴氰虫酰胺悬浮种衣剂对稻蓟马的防治效果

[目的]筛选防治稻蓟马的有效药剂.[方法]开展了40％噻虫嗪·溴氰虫酰胺FS防治稻蓟马的田间药效试验.[结果]药后15d,40％噻虫嗪·溴氰虫酰胺FS各剂量处理对稻蓟马的防效为98.42％～ 99.20％,药后30 d,40％噻虫嗪·溴氰虫酰胺FS各剂量处理对稻蓟马的防效为93.79％ ～ 99.29,药后40d的杀虫防效为32.83％ ～ 92.89％.[结论]40％噻虫嗪·溴氰虫酰胺FS的适宜用量为96g/100kg.     

贵族式的张扬与沉落——《上海宝贝》与《莎菲女士的日记》比较

莎菲和倪可作为知识女性,她们都曾自觉不自觉地或者说有意无意地追求“贵族文化”生活,向往一种贵族境界、诗意人生。但她们的爱情观念及其实践活动却有很大不同:莎菲冷静却又软弱,坚守爱情价值的张扬;而倪可虽有成就女性的表象,却在实际生活中消解了女性,甚至消解了人性。     

刺槐素对禽源性大肠杆菌的抑菌作用及耐药性消除的研究

该试验研究刺槐素对鸡源性大肠杆菌的抑菌作用和耐药性消除效果。采用药敏纸片判断刺槐素的抑菌效果,对比前后2次MIC的浓度大小判断刺槐素对大肠杆菌的耐药性消除效果。结果:刺槐素抑菌效果:大肠杆菌①的MIC为32μg/mL,大肠杆菌②的MIC为128μg/mL,大肠杆菌③的MIC为64μg/mL,大肠杆菌④的MIC为16μg/mL,大肠杆菌⑤的MIC为32μg/mL。耐药性消除试验结果:大肠杆菌①的MIC由1024μg/mL下降为128μg/mL,大肠杆菌②的MIC由512μg/mL下降为64μg/mL,大肠杆菌③的MIC由2048μg/mL下降为128μg/mL,大肠杆菌④的MIC由256μg/mL下降为32μg/mL,大肠杆菌⑤的MIC由512μg/mL下降为64μg/mL。结论:刺槐素对鸡源性大肠杆菌具有抑制作用,对头孢耐药性有消除作用。     

Gradients of ozone at a forest site and over a field cropconsequences for the AOT40 concept of critical level

Ozone concentrations were measured at a wind-exposed edge of a 60 year-old 15–20 m tall Norway spruce forest in south-west Sweden and simultaneously over a barley field 5 km away for 27 days, At the forest site, measurements were performed at 3 and 13 m height 15 m in front of the forest edge, at 3 m height 15 m into the forest, and at 3 and 13 m height 45 m into the forest. Measurements at 3 m were made with three replicate tubes separated by 10 m. Differences between replicates were small. At 13 m height, the concentration (24-hr-average) 45 m into the forest was 95% of that in front of the forest edge. The average concentration at 3 m height did not vary strongly with the distance into the forest, but was 86% of that at 13 m in front of the forest edge. For AOT40 (Accumulated Exposure Over Threshold 40 ppb ozone), the differences between different positions were larger. At the 13 m level the AOT40 (day and night) was 88% of that in front of the forest 45 m into the forest. The AOT40 at 3 m was 71% of that at 13 m outside the forest. At the crop site, the ozone concentration at 1.1 m (0.1 m above the canopy), was 78% of that at 9 m (06.00–22.00). The AOT40 at 1.1 m above the ground, however, was only 50% of that at 9 m, indicating that serious errors can arise if ozone monitoring data are used uncorrnected in dose-response relationships based on measurements performed at plant height. The ozone concentration for the whole period differed very little between 9 m height at the crop site and 13 m height at the forest site outside the forest during daytime conditions (06.00–22.00). Night-time (22.00–06.00) values were only 21% at the crop site of those at the forest site due to the stronger night inversion development in the agricultural environment compared to the wind exposed forest edge. The results suggest that variations in topography and vegetation are important to consider when combining ozone monitoring data with dose-response functions.     

优质玉米杂交种丹玉40号的选育及应用

丹玉40号(丹3040)是丹东农业科学院以自选系丹SE30为母本,自选系丹SE79-1为父本组配成的玉米杂交种。该品种属晚熟杂交种,需≥10℃有效积温,积温2 381.4℃左右。1993～2000年在各级各类试验中表现高产、稳产、抗病、抗倒、适应性强。一般比各地主栽品种增产8％以上。适宜在吉林南部、辽宁大部及华北春玉米区种植。种植密度以45 000株/hm~2为宜,比空和清种均可。     

Antioxidant effect of dietary tocopherol and ascorbic acid on growth and survival of Litopenaeus vannamei postlarvae

The nutritional effect of vitamin E in dietsfor Litopenaeus vannamei postlarve (PL19)was investigated. Four formulated diets withdifferent combinations of -tocopherylacetate (-TA), ascorbic acid (AA) andhighly unsaturated fatty acids (HUFA) weretested, using four replicates.No significant differences in survival wereobserved among treatments after 34 days offeeding. However, shrimp fed with a dietcontaining 2% fish oil (low n-3 HUFA content),200 mg.kg^–1 -TA and100 mg.kg^–1 AA (diet H/E/C) showedsignificantly better growth than those fed adiet supplemented with 5% fish oil (high n-3HUFA content), 200 mg.kg^–1 -TA and100 mg.kg^–1 AA (diet H+/E/C). Shrimp fedwith a diet containing 5% fish oil,900 mg.kg^–1 -TA and100 mg.kg^–1 AA (diet H+/E+/C) showed a significantly higher tissue level of n-6 PUFAthan postlarvae fed diet H+/E/C. No definiteconclusion could be drawn about a possibleinteraction between -TA and AA, since acomparison of the diet containing 5% fish oil,200 mg.kg^–1 -TA and700 mg.kg^–1 AA (H+/E+/C+) and the dietH+/E/C did not show any significant differencesin any of the measured parameters. Theantioxidative status of the shrimp tissue(measured by means of the thiobarbituric acid(TBA) assay and expressed as nM malonaldehyde(MA) per gramme dry weight) was equal for alltreatments. Nevertheless, there was a slightlylower MA value with the diet H+/E/C+,indicating that AA may be an effectiveantioxidant in the aqueous phase and at thewater/lipid interface of the tissue. The tissuelevels of -T and AA were highlydependent on the amounts in diets and nocorrelation between -T and AAincorporation could be observed.