过表达一个疣粒野生稻的Ricin_B凝集素基因OmR40c1增强水稻耐盐性

盐胁迫是影响盐/碱稻区水稻产量的主要逆境因素。研究耐盐相关基因,对于培育水稻抗逆品种具有重要意义。OmR40c1是从疣粒野生稻(Oryza meyeriana)中分离获得cDNA序列,与已报道的受脱落酸与盐诱导的OsR40c1的CDS序列完全相同,但在5′端UTR和3′端UTR区域有差别。有关OmR40c1基因功能的研究还未见报道。本研究的主要目的是初步分析OmR40c1在水稻耐盐性中的作用。对栽培稻日本晴(Oryza sativa L.var.Nipponbare)进行脱落酸(abscisic acid,ABA)和盐胁迫处理,OsR40c1基因均上调表达。构建基因定位载体并注射烟草表皮细胞,其结果表明OmR40c1定位于细胞质膜和细胞核上。构建超表达载体并转染日本晴,PCR及qRT-PCR结果均显示OmR40c1在转基因材料中成功表达。OmR40c1可能参与种子萌发。174mmol/L盐胁迫下,野生型种子的发芽率下调一半左右,转OmR40c1基因水稻种子发芽未受影响;转OmR40c1水稻苗期的株高是未经盐处理转基因水稻株高的一半左右;而野生型的株高是未经盐处理野生型水稻株高的1/6左右。盐胁迫下,水稻叶片、根长等都受到不同程度的影响。盐胁迫下,转OmR40c1基因水稻植株内的Na~+浓度是野生型的1.38倍;K~+浓度是野生型的1.25倍。综上,OmR40c1一定程度提高了水稻的耐盐性,且苗期的耐盐性高于成株期。     

日本血吸虫WD40信号蛋白的原核表达

本研究克隆了编码日本血吸虫WD40信号蛋白的cDNA,并分析了WD40在日本血吸虫不同发育时期的转录情况.同时还利用原核表达系统对该蛋白的部分序列进行了表达,并进行了重组蛋白的纯化和多克隆抗体的制备,Western blot分析表明本研究中获得多克隆抗体可与重组表达的蛋白和血吸虫全虫蛋白裂解物具有特异性反应.本试验结果为进一步研究WD40信号分子的功能奠定了初步基础.     

Retarding effect of simvastatin on artery remodeling induced by high-salt and high-fat diet in rats

AIM: To investigate the effect of simvastatin intervention on the changes of blood pressure, serum lipid fluctuation and aortic configuration induced by high-sodium and high-fat diet in rats. METHODS: Sixty adult male SD rats were randomly divided into 5 groups (n=12): control (N)group, high salt (S)group, high fat (F) group, high salt+ high fat (SF) group and high salt+high fat + simvastatin (T) group. After fed for 16 weeks, the rats were subject to determine blood pressures and serum concentrations of triglycerides (TG),total cholesterol(TC) and soluble CD40 ligand (sCD40L). The expression of CD40/CD40L in the root of ascending aorta was detected by immunohistochemical method. The thickness of intima media in the ascending aorta as well as the ratio of lumen area/total vascular area were measured and calculated after HE staining. RESULTS: In S group, F group and SF group, systolic blood pressure was significantly higher than that in N group (P<0.01). Systolic blood pressure in T group were slightly higher than that in N group with statistical significance and significantly lower than that in SF group. The serum concentrations of TG and TC in F group and SF group were significantly higher than those in N group and T group (P<0.01), and no significant difference among S group, N group and T group was observed. In S group, F group and SF group, the serum concentrations of sCD40L were higher than that in N group and T group (P<0.05), meanwhile that in SF group was also higher than that in S group and F group (P<0.05). However, no significant difference of sCD40L concentration between S group and F group as well as N group and T group was observed. The expression of CD40/CD40L in the ascending aorta in S group, F group and SF group was higher than that in N group and T group (P<0.05), and that in SF group was also higher than that in S group and F group (P<0.05).No significant difference of CD40/CD40L expression between S group and F group as well as N group and T group was observed. The thickness of intima media in S group, F group and SF group was significantly thicker than that in N group (P<0.01), and no significant difference of the intima media thickness between T group and N group was observed. The ratio of lumen area/total vascular area in S group, F group and SF group was smaller than that in N group (P<0.05), and no significant difference of the ratio between T group and N group was found. CONCLUSION: Feeding high-fat and high-salt diet leads to blood pressure elevation, induces atherosclerosis, increases serum concentration of sCD40L and enhances the expression of CD40/CD40L in arterial tissues. The combination of the stimuli has stronger effect than a single factor. Statins protect the arterial tissues against atherosclerosis by decreasing the level of serum sCD40L and inhibiting the arterial expression of CD40/CD40L.     

北京西瓜产业发展40年来回顾及展望

西瓜作为北京市的主要农作物,是农民增收致富的重要产业.1980年至今北京西瓜产业经历了提高总产保供应、优质化多样化、全面提升和绿色高质量发展四个阶段.笔者回顾了40年来北京西瓜产业发展的四个阶段,总结了发展中存在的问题,提出了发展对策,并对北京西瓜产业发展进行了展望.     

苏博美利奴羊胚胎期WNT2基因的组织表达及其生物信息学预测分析

【目的】检测WNT2基因在苏博美利奴羊胚胎期135 d不同组织中的表达水平,了解WNT2基因的分子结构和进化特征,从分子水平及进化特征上研究苏博美利奴羊的组织器官与WNT2基因表达之间的内在联系,为筛选细毛羊毛囊发育相关候选基因提供理论依据。【方法】采用qRT-PCR法研究苏博美利奴羊毛囊成熟时期WNT2基因在不同组织中的表达,并使用PROMO软件预测WNT2基因启动子区域序列的转录因子,并用Cytoscape_v3.5.1软件可视化,使用MEGA 7.0软件分析WNT2基因启动子区域序列的核苷酸组成成分及密码子的偏好性,并且利用绵羊、山羊、牛、人等9个物种的WNT2基因的DNA序列构建系统进化树。【结果】WNT2基因在皮肤组织中的表达量极显著高于心脏、肝脏、脾脏、肺脏、肾脏及肌肉组织。在绵羊WNT2基因启动子区域序列共预测出101个相关转录因子,4种碱基呈现均匀分布,CAG与UCU是使用相对较多的密码子,对WNT2基因的DNA序列系统进化树分析发现山羊和绵羊之间的进化关系比与其他哺乳动物进行比较时更为接近。【结论】建立了胚胎期135 d的苏博美利奴羊的不同组织中WNT2基因表达量的RT-PCR方法,并使用生物信息学软件对其分子结构和进化特征进行分析。     

紫色番茄SlWD40-like基因克隆与表达分析

WD-重复蛋白(WD-repeat protein)是一类含有WD40基序的蛋白,在植物响应非生物逆境胁迫的过程中具有重要调控作用。为揭示紫色番茄中WD40重复蛋白的功能,从富含花青素紫色番茄品种‘砚紫1号’中克隆 SlWD40-like基因,并对该基因在不同组织部位和不同胁迫条件下的表达模式进行分析。结果表明, SlWD40-like基因编码1个含有460个氨基酸的蛋白质。蛋白序列分析显示,SlWD40-like蛋白含有6个WD40基序,是典型的WD40重复蛋白。预测分析发现,该蛋白为亲水性蛋白,其二级结构以无规则卷曲为主;该蛋白序列在物种间具有高度的保守性,系统发生进化树分析表明紫番茄SlWD40-like氨基酸序列与潘那利番茄蛋白亲缘关系最近,其次是胡萝卜。应用qRT-PCR分析 SlWD40-like基因组织特异表达发现,该基因在叶和紫色果实中表达量最高。qRT-PCR分析表明, SlWD40-like基因受到盐胁迫、干旱胁迫脱落酸(ABA)和茉莉酸甲酯(MeJA)诱导表达,推测该基因可能参与紫色番茄逆境胁迫应答。该研究为后续进一步分析紫色番茄 SlWD40-like基因功能奠定了基础。     

大花蕙兰授粉后子房cDNA差异表达片段序列分析

 应用mRNA差异显示(DDRT-PCR) 方法比较分析了大花蕙兰(Cymbidium hybridium ) 授粉后2 d与未授粉子房的基因表达差异。结果分离到了7个在授粉后子房中特异表达的cDNA片段, 分别命名为CDD-313, CDD-272, CDD-265, CDD-243, CDD-193, CDD-218,CDD-470。在GenBank中进行同源性比较发现前4个没有同源序列与之匹配; 后3个片段推导的氨基酸序列分别与ABC型transporter, GTPase和40S核糖体蛋白质S3 (RPS3) 有高度同源性。反向Northern杂交进一步证实它们存在, 并在授粉子房中高度表达。其中, 最为有意义的是CDD-470, 其推定的氨基酸与参与细胞生长分化的植物源多功能蛋白RPS3高度同源性, 进而推测其与RPS3有相似的功能, 可能与兰花子房发育有关, 这为解释花发育分子机制提供了新资料。     

A vaccine formulation combining rhoptry proteins NcROP40 and NcROP2 improves pup survival in a pregnant mouse model of neosporosis

Currently there are no effective vaccines for the control of bovine neosporosis. During the last years several subunit vaccines based on immunodominant antigens and other proteins involved in adhesion, invasion and intracellular proliferation of Neospora caninum have been evaluated as targets for vaccine development in experimental mouse infection models. Among them, the rhoptry antigen NcROP2 and the immunodominant NcGRA7 protein have been assessed with varying results. Recent studies have shown that another rhoptry component, NcROP40, and NcNTPase, a putative dense granule antigen, exhibit higher expression levels in tachyzoites of virulent N. caninum isolates, suggesting that these could be potential vaccine candidates to limit the effects of infection. In the present work, the safety and efficacy of these recombinant antigens formulated in Quil-A adjuvant as monovalent vaccines or pair-wise combinations (rNcROP40 + rNcROP2 and rNcGRA7 + rNcNTPase) were evaluated in a pregnant mouse model of neosporosis. All the vaccine formulations elicited a specific immune response against their respective native proteins after immunization. Mice vaccinated with rNcROP40 and rNcROP2 alone or in combination produced the highest levels of IFN-γ and exhibited low parasite burdens and low IgG antibody levels after the challenge. In addition, most of the vaccine formulations were able to increase the median survival time in the offspring. However, pup survival only ensued in the groups vaccinated with rNcROP40 + rNcROP2 (16.2%) and rNcROP2 (6.3%). Interestingly, vertical transmission was not observed in those survivor pups immunized with rNcROP40 + rNcROP2, as shown by PCR analyses. These results show a partial protection against N. caninum infection after vaccination with rNcROP40 + rNcROP2, suggesting a synergistic effect of the two recombinant rhoptry antigens.     

40%毒死蜱·噻嗪酮乳油EC防治稻飞虱的效果

田间试验结果表明,40%毒死蜱·噻嗪酮乳油90mL处理对水稻稻飞虱有良好的防治效果,药后15d,防效仍为90.8%,且对水稻生长安全。     

水稻库/源比对叶片光合作用、同化物运输和分配及叶片衰老的影响

两系杂交稻Ｎ３１ＳＳ／Ｐ４０水培稻株不同库／源比值株剑叶光合速率在灌浆结实前期去叶处理明显高于对照,去１／２花则降低光合速率;灌浆中,后期处理间差异较小。改变库／源比后１－７去,去叶处理剑叶的ＲｕＮＢＰ羧化酶活性,光合磷酸化和Ｈｉｌｌ反庆活性,蔗糖磷酸合成酶活性,叶片中无机磷含量,被同化碳在醇溶部分分配比例及光合同化物从剑叶输出的速率均明显高于对照,而叶片中蔗糖和淀粉含量低于对照。