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131.
齿轮三维模型的参数化设计技术 总被引:1,自引:0,他引:1
齿轮是汽车、拖拉机中常用的传动零件,在三维设计中,需要对渐开线方程进行转换,由转换后的方程生成齿形,其整个设计过程非常繁琐。本文详述了方程的转换过程及齿轮的参数化设计方法,可以通过输入不同的齿轮参数,即可获得相应的齿轮,从而大大节约设计时间。该方法还可应用于所有系列化的通用件和标准件三维设计中。 相似文献
132.
采用三维弹塑性非线性有限元法,对云龙河拱坝施工过程的实际浇筑、封拱和水库蓄水过程的坝体应力及变形进行了仿真分析,同时分析了施工过程的坝肩层间错层带的变形。计算结果表明施工过程的坝体变形较小,坝体有限元等效应力满足规范要求,坝肩层间错层带错动变形很小,说明大坝结构设计合理,坝肩无错动危险。计算成果为工程项目的实施提供了技术依据,同时对类似工程分析具有借鉴作用。 相似文献
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134.
LAI Ying-yu GAO Xiao-yan ZHOU Hui-shan LI Xin WANG Zhao-yu PENG De-wei RAO Fang DENG Chun-yu 《园艺学报》2021,37(1):18-26
AIM To investigate the role of p300 in aging-related atrial fibrosis in human atrial fibroblasts (HAFs) and its potential mechanism. METHODS HAFs were obtained from human left atrial tissue, and the senescence model was established by cell passage. Senescence-associated β-galactosidase (SA-β-Gal) staining was used to detect the cell senescence, and Western blot was used to determine the protein levels of p300, p53, Smad3 and other senescence and fibrosis associated proteins in HAFs. RESULTS Compared to passage 3 HAFs, the proportion of senescent cells, and the protein levels of p300, p53, p-Smad3 and other senescence and fibrosis associated proteins were increased in HAFs at passage 7 and 11 (P <0.05). After treated with curcumin (a p300 inhibitor) or transfection with p300 small-hairpin (sh) RNA plasmid, the protein levels of p300, and the senescence and fibrosis associated proteins were decreased in HAFs at passage 7(P <0.05). Up-regulation of p300 by transfection with p300 over-expression plasmid increased the protein levels of p53, Smad3 and MMP-2 in HAFs at passage 3 (P <0.05). CONCLUSION p300/p53/Smad3 signaling pathway plays an important role in aging-related atrial fibrosis in HAFs. 相似文献
135.
AIM To observe the effect of naringenin on cardiac injury in ischemia/reperfusion (I/R) rats, and to explore whether the role of naringenin is involved in PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways. METHODS SD rats (n =48) were randomly divided into sham operation (sham) group, model (I/R) group, naringenin treatment (NAR) group and naringenin+LY294002 (NL) group. Myocardial I/R injury model was prepared by ligation of left anterior descending coronary artery of rats for 30 min followed by reperfusion for 120 min. After reperfusion, the serum levels of cardiac troponin I (cTnI) was measured by ELISA. HE staining, TTC staining and TUNEL staining were used to detect the myocardial histopathological changes, myocardial infarction area and myocardial cell apoptotic rate. The mRNA levels of endoplasmic reticulum stress-related indicators glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP) and caspase-12 were detected by RT-qPCR. The protein levels of cleaved caspase-3, GRP78, CHOP, caspase-12, p-PI3K and p-AKT were determined by Western blot. RESULTS Compared with I/R group, the serum content of cTnI, myocardial pathological damage, myocardial infarction area and myocardial cell apoptotic rate were significantly reduced (P <0.05), the protein levels of cleaved caspase-3, GRP78, CHOP and caspase-12 were decreased (P <0.05), and the protein levels of p-PI3K and p-AKT were increased in NAR group (P <0.05). LY294002 attenuated the protective effect of naringenin to some extent. CONCLUSION Naringenin reduces myocardial I/R injury in rats possibly by activating PI3K/AKT signaling pathway and subsequently regulating endoplasmic reticulum stress and its related apoptotic pathways. 相似文献
136.
1990年和1991年对127个已定型的春大豆株系的抗大豆花叶病毒病的抗性及产量进行了测定.其中高抗材料有51个,中抗材料19个.在高抗材料中,有7个株系比对照增产10%以上,是很有希望的新品系,有15个材料病指在1.0以下,但产量表现不突出,可作为抗源材料,根据不同地区同一品种抗性差异较大这一结果,抗性自然鉴定圃宜设在大豆病毒病重病区. 相似文献
137.
Godelieve Gheysen Walter Van der Eycken Nathalie Barthels Mansour Karimi Marc Van Montagu 《Pest management science》1996,47(1):95-101
The molecular interactions between plants and sedentary nematodes are undergoing intense study, not only for reasons of fundamental research but also for the potential benefits to agriculture. The present technology allows the transformation of an increasing number of crop plants, providing new ways to introduce resistance against plant-parasitic nematodes. The ability of sedentary nematodes to induce specialized feeding sites in plant roots is one of the most fascinating aspects of this host–parasite interaction. Molecular approaches have been initiated to identify and characterize plant genes altered in expression after infection by sedentary nematodes. The results obtained indicate that many genes indeed become up-regulated upon nematode infection. Surprisingly, several so-called constitutive promoters that are normally used to achieve high expression in plant cells are completely ‘silenced’ in the feeding sites within days after nematode infection. Generally, there are two options available for the genetic engineering of nematode resistance: the synthesis of anti-nematode proteins or the localized production of a cytotoxic protein that interferes with the development of feeding cells. Nematode-induced promoters are very useful for the production by plants of sufficiently high levels of anti-nematode proteins at feeding sites. Alternatively, interfering with feeding-cell development is somewhat similar to the hypersensitive response evoked by nematodes in a naturally resistant plant. Here, destruction of specific plant cells can be achieved by the localized expression of a cytotoxin such as barnase, a potent ribonuclease. This approach, however, calls for a highly specific ‘non-leaky’ promoter, which is active only in the feeding cells. Another possibility is to use a two-component system, where the leakiness of the promoter in other tissues is counterbalanced by the constitutive expression of a neutralizing gene. 相似文献
138.
Andrew Plant Achim Harder Norbert Mencke Heinz-Jürgen Bertram 《Pest management science》1996,48(4):351-358
7-Hydroxy-5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thio-carboxanilides represent a novel series of anthelmintic compounds, with broad-spectrum activity against important parasitic nematodes in sheep and dogs. In particular, an improved efficacy against Trichostrongylus colubriformis in sheep over the related 3-carbamoyl-4-hydroxycoumarins has been noted. New synthetic routes to the key intermediate, 7-hydroxythieno[3,2-b]pyran-5-one, have been developed. 相似文献
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140.