香蕉ACC氧化酶基因(MAO3)的克隆及其表达特性分析

 根据同源扩增得到香蕉(Musa acuminata) ACC氧化酶基因(MAO3) 的核心部分, 再通过3'和5'RACE扩增上、下游序列以及Genome-Walker的方法得到启动子部分, 共获得3 718 bp长度的序列。将所得结果进行聚类分析, 发现香蕉中ACC氧化酶的氨基酸序列非常保守, 各序列间的同一性高达99% ,与单子叶植物和双子叶植物中ACC氧化酶的氨基酸序列的同源性在66.7%～71.8%之间。组织原位杂交试验表明MAO3基因的表达具有组织特异性, 初步认为是在韧皮部筛管组织中特异表达。运用实时荧光定量PCR技术, 实时监测到了MAO3基因和香蕉乙烯受体基因ERS2受机械伤诱导的定量变化。     

大青叶中4（3H）喹唑酮体外抗H1N1型流感病毒的作用研究

目的：探讨大青叶中4（3H）喹唑酮体外抗H1N1型流感病毒的作用。方法：用MTT染色法测定大青叶中4（3H）喹唑酮通过不同加药方式对H1N1型流感病毒的体外抑制作用。结果：大青叶中4（3H）喹唑酮对H1N1型流感病毒具有明显的阻断作用和对细胞内病毒有抑制作用。结论：大青叶中4（3H）喹唑酮具有明显的体外阻止H1N1型流感病毒吸附到靶细胞和抑制H1N1型流感病毒增殖的作用。     

Genetic Variability in Phosphorus Acquisition and Utilization Efficiency from Sparingly Soluble P-Sources by Brassica Cultivars under P-Stress Environment

Plants display an array of classical strategies to maximize phosphate (Pi) acquisition from sparingly soluble P sources. Acclimation to Pi-stress via elegant Pi-starvation induced (PSI) adjustments would reduce our current overreliance on expensive, polluting and non-renewable Pi-fertilizers. Nevertheless, differences in the ability of various species to solubilize sparingly soluble P-sources have been often evidenced; inter-cultivar variations are scarcely documented. Brassica is known as an effective, non-mycorrhizal user of sparingly soluble P-sources. Various growth parameters and biomass accumulation by genetically diverse Brassica cultivars were determined in four experiments using hydroponics and quartz sand culture media. Role of PSI root mediated pH changes, organic anions (OAs) exudation and altered root architecture in mobilization and acquisition of sparingly soluble P-forms [Jordan rock-P (RP) at 2 g l⁻¹ and Ca₃(PO₄)₂ (TCP) at 0.2 g l⁻¹ respectively] was investigated. Cultivars showed considerable genetic variations in biomass accumulation, various growth parameters and root–shoot ratio. Concentration and total uptake of P, specific absorption rate of P, P-transport rate and P-utilization efficiency (PUE) were also significantly (P < 0.001) different for various cultivars and their dry matter was significantly correlated with P-uptake [r = 0.94** (significant at 1% level)]. P-tolerant cultivars showed substantial decrease in solution media pH because of H⁺ efflux and exuded more carboxylates than low P-sensitive cultivars under P-starvation. P-uptake by cultivars increased linearly with decreasing pH. The amount and types of OAs exuded from the roots of P-starved plants differed from those of plants grown under P-sufficient environment. In split pot study, with TCP and RP supplied spatially separated from other nutrients, efficient cultivars were still able to mobilize RP and TCP more efficiently than inefficient cultivars. In rhizobox study, the elongation rates of primary roots decreased but the elongation rates of the branched zones of primary roots and the length of lateral roots increased under P-starvation. Tested cultivars showed genetic diversity in accessing, mobilization, acquisition and utilization of Pi from sparingly soluble P forms. An arrange marriage of plant traits can explain cultivar’s access to different forms of sparingly soluble P, and in addition to altered lateral root topology and enhanced P-uptake and PUE, enhanced H⁺ efflux and OAs exudation are key factors in Pi scavenging from extra cellular sparingly soluble P-forms.     

AgNO_3对香石竹切花保鲜效应的研究

研究了不同浓度的AgNO3对香石竹切花的保鲜效应。所用的基本保鲜液为20g/L蔗糖+200mg/L 8-羟基喹啉(8-HQ)+100mg/L柠檬酸,并以此为对照,分别加入不同浓度的AgNO3,观察切花的形态结构,并测量切花鲜重、花径及电导率等。结果表明:各种浓度AgNO3处理均能不同程度影响切花观赏品质,延缓切花衰老进程,其中以30mg/LAgNO3处理效果最佳。     

贺兰山主峰哈拉乌沟流域三水转化关系探讨

贺兰山主峰哈拉乌沟流域位于阿拉善左旗巴音浩特镇东侧,沟内1977年为旗镇建截潜流供水水源地。根据1979年~1987年实际观测沟域降水,地表水和潜流资料,研究提出三水转化参数,可为内蒙古半干旱山丘区开发利用沟谷潜水进行水资源评价参考。     

基于“3S”的甘肃省苜蓿病害诊断专家系统的知识获取与表示

知识获取和知识表示是构建专家系统的核心环节。在知识获取方面采用了基于“3S”（地理信息系统GIS、遥感RS、全球定位系统GPS）的半自动获取方式，这一方式结合了人工获取和自动获取的优势，提高了苜蓿Medicago spp.病害诊断的准确性。同时，应用“3S”技术把传统的关系数据库与具有空间属性的矢量数据关联起来，生成具空间属性的新知识库，增强了苜蓿病害诊断的可靠性，更加有利于苜蓿病害知识库的及时更新。在知识表示方面，则采用传统的产生式(If-Then)表示法，表示方式自然、简洁，易于广大基层农业人员所理解和接受，增强了苜蓿病害诊断专家系统实用性，有利于苜蓿病害诊断专家系统的推广应用，进而促进苜蓿产业的快速发展。     

关于使用 Pro/Engineer wildfire软件设计三维机械零件的几点技巧在教学中的探讨

本文对使用Pro/Engineer Wildfire 软件设计三维机械零件在教学中遇到的若干问题作了深入细致的分析,并提出了相应的解决技巧.     

赤霉素对发育的大豆种子中同化物卸出和积累的影响

用微注射法将不同浓度的赤霉素 (GA3 )引入开花后 30 d的大豆种子幼胚的 2片子叶中间的质外体空间 ,同时给叶片饲喂 14 CO2 ,以种皮和子叶中放射性强度来比较分析 GA3 对同化物卸出的影响。分析了种皮和子叶中的酸性转化酶活性 ,发现 GA3 1× 10 -5mol.L-1使种皮中酸性转化酶活性提高而子叶中酶活性降低 ,从而促进种皮同化物的卸出和向子叶的分配。实验结果还表明 ,引入 GA3 影响了种子内源脱落酸 (ABA)水平 ,而且子叶中的 ABA含量变化和同化物积累相关 ,GA3 1× 10 -5mol.L-1使子叶中 ABA水平上升 ,伴随总糖、蔗糖和蛋白质的积累增加 ,GA3 1× 10 -4 mol.L-1却使 ABA水平下降 ,而且物质积累也减少     

Comparison of sampling and isolation procedures for recovery of Yersinia enterocolitica serotype O:3 from the oral cavity of slaughter pigs

Yersinia enterocolitica serotype 0:3/biotype 4 was isolated from the oral cavity of altogether 32 (68.1 %) of 47 freshly eviscerated slaughter pigs. Most efficient recovery was achieved by cultivation of tissue samples from both tongue and tonsils of the same individual. The isolation rate so obtained was significantly higher than that obtained by separate examination of either tonsil swabs or tongue swabs. However, the isolation frequency achieved by combined swabbing of the 2 sites was not significantly different. In general, tonsils were more productive for the recovery of 0:3 strains than were tongues, and tissue samples yielded higher isolation rates than did swabs. Three-week cold enrichment in a low selective medium proved essential for optimal recovery. However, the highest number of isolates was obtained using a combination of methods, including direct plating and selective enrichment in a modified Rappaport broth in addition to cold enrichment.