枸杞多糖对双酚A暴露小鼠生精细胞Caspase-3、Bcl-2和Bax蛋白表达的影响

研究枸杞多糖(LBP)对双酚A(BPA)暴露小鼠睾丸生精细胞中Caspase-3、Bcl-2和Bax凋亡蛋白表达的影响.将50只成年雄性昆明小鼠随机分为A、B、C、D、E共5组,每组10只.除正常对照组(A组)注射等量橄榄油外,其余4组小鼠分别腹腔注射20 mg·kg 1的BPA,连续7d,建立生精损伤模型.同时C、D、E组小鼠分别灌服7d不同剂量的LBP(50、100、200 mg·kg-1),正常对照组(A组)和模型组(B组)小鼠灌服等量生理盐水.制备组织切片观察睾丸组织病理学变化,免疫组化法测定睾丸组织Caspase-3、Bax和Bcl-2凋亡蛋白的表达.结果显示,BPA可极显著增加睾丸生精细胞Caspase-3和Bax的阳性细胞数量(P＜0.01),降低Bcl-2的表达(P＜0.05).补充不同剂量LBP后,Caspase-3的阳性表达均极显著低于模型组(P＜0.01).200 mg·kg-1 LBP组生精细胞Bax的阳性细胞数量极显著低于模型组(P＜0.01);Bcl-2的表达随LBP剂量的增加而提高,其中200 mg·kg1LBP组阳性表达极显著高于模型组(P＜0.01),Bcl-2/Bax比值也随着LBP剂量的增加而上升.结果表明,枸杞多糖通过调节凋亡相关基因的表达,抑制生精细胞凋亡,从而缓解双酚A引起的雄性生殖损伤.     

Two anatomic resources of canine pelvic limb muscles based on CT and MRI

Advances in magnetic resonance (MR) imaging and three-dimensional (3D) modeling software provide the tools necessary to create sophisticated, interactive anatomic resources that can assist in the interpretation of MR images of extremities, and learning the structure and function of limb musculature. Modeling provides advantages over dissection or consultation of print atlases because of the associated speed, flexibility, 3D nature, and elimination of superimposed arrows and labels. Our goals were to create a diagnostic atlas of pelvic limb muscles that will facilitate interpretation of MR images of patients with muscle injury and to create a 3D model of the canine pelvic limb musculature to facilitate anatomic learning. To create these resources, we used structural segmentation of MR images, a process that groups image pixels into anatomically meaningful regions. The Diagnostic Atlas is an interactive, multiplanar, web-based MR atlas of the canine pelvic limb musculature that was created by manually segmenting clinically analogous MR sequences. Higher resolution volumetric MR and computed tomography (CT) data were segmented into separately labeled volumes of data and then transformed into a multilayered 3D computer model. The 3D Model serves as a resource for students of gross anatomy, encouraging integrative learning with its highly interactive and selective display capabilities. For clinicians, the 3D Model also serves to bridge the gap between topographic and tomographic anatomy, displaying both formats alongside, or even superimposed over each other. Both projects are hosted on an open-access website, http://3dvetanatomy.ncsu.edu/     

Effects of tris(1,3-dichloro-2-propyl) phosphate on epididymal sperm parameters in adult male rats

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP.     

Closing wedge ostectomy and medial distal tibia plate application for treatment of severe bilateral angular limb deformities in the hindlimbs of an 8-month-old foal

This case report describes the evaluation, surgical approach and outcome of an 8-month-old foal with severe angular limb deformities of both metatarsophalangeal joints (MTPJ). Radiography and computed tomography (CT) were used to diagnose a windswept deformity of the hindlimbs with associated bilateral physitis. The orthopaedic surgery was planned and rehearsed using three-dimensional (3D) bone models. A bilateral closing wedge ostectomy was performed on the distal MtIII, and 3.5 mm LCP^® medial distal tibia plates were used bilaterally for osteosynthesis. Even though convalescence was prolonged and complicated by partial unilateral implant failure and osteoarthritis (OA), reduction of the deformities was successful and resulted in a sound foal.     

油酸和硬脂酸对奶牛乳腺上皮细胞中脂肪酸结合蛋白3表达和脂滴分泌的影响

为确定外源添加长链脂肪酸油酸和硬脂酸对奶牛乳腺上皮细胞中脂肪酸结合蛋白3（FABP3）表达的影响,本试验采用荧光定量RT-PCR检测乳脂合成相关基因的表达情况,确定油酸和硬脂酸的最佳浓度和培养时间分别为75 μmol/L 12 h和100 μmol/L 36 h。采用Western blotting和免疫荧光法检测添加油酸和硬脂酸后FABP3表达和脂滴分泌的情况,为进一步揭示FABP3在乳脂合成信号转导通路中的作用提供理论依据。     

三维CAD技术在机械设计中的应用

现代计算机在技术和性能上越发成熟,再加之网络的普及和信息的智能化处理技术的提高,三维CAD技术本身也正逐步成熟,因此,正确地把握CAD技术的发展趋势对我国机械工程中的设计工作具有深远的意义.     

猪血清中天然LFA－3存在的探讨

测定了正常猪血清对ＳＲＢＣ的溶血作用。并根据猪血清对ＳＲＢＣ的破坏作用经ＳＲＢＣ吸收和加入ＳＲＢＣＬＦＡ－３后即显著降低或消失，提出了猪血清破坏ＲＢＣ的作用与血清中游离ＣＤ＿２的存在有关的论断。此外，又根据反复解离和经ＳＲＢＣ吸收后的猪血清具有阻止正常猪血清对ＳＲＢＣ的破坏作用和猪ＰＢＭＣＥ玫瑰花环的形成，认为猪血清中可能存在天然的ＬＦＡ－３，进而对血清游离ＣＤ＿２在淋巴细胞激活中的功能提出了“游离ＣＤ＿２调控途径”的设想。     

Effects of Ω-3 (n-3) Fatty Acid Supplementation on Insulin Sensitivity in Horses

The objective of this study was to examine the effects of dietary ω-3 fatty acid supplementation on insulin sensitivity (SI) in horses. Twenty-one mares were blocked by age, body weight (BW), and body condition score (BCS) and randomly assigned to one of three dietary treatments. Treatments consisted of (1) 38 g of n-3 fatty acids via fish and algae supplement and diet (MARINE), (2) 38 g of n-3 fatty acids via a flaxseed meal from the supplement and diet (FLAX), and (3) control (CON) no supplemental fatty acid. Treatments were supplemented for 90 days. Frequent sampling intravenous glucose tolerance tests were performed on days 0, 30, 60, and 90. Blood samples were analyzed for glucose and insulin. The minimal model was applied for the glucose and insulin curves using MinMod Millennium. SI increased 39% (P < .007) across all treatment groups. Acute insulin response to glucose decreased 22% (P < .006) between days 30 and 60 and increased (P = .040) again at day 90. Disposition index (combined SI and β pancreatic response) increased (P = .03) by 53% in the MARINE- and 48% in the FLAX-supplemented horses and did not change with time in the CON group. In insulin-resistant mares, MARINE- and FLAX-treated horses had an increase in SI (P = .09). It would be interesting to test this supplement in a larger group of insulin-resistant horses. If proven effective, supplementation with ω-3 fatty acids would help to reduce problems associated with insulin resistance in horses.