甘蓝型油菜花茎高效再生体系研究

研究了甘蓝型油菜花茎外植体高效诱导不定芽的再生体系,结果表明:以含有2,4-D(0.5￣1.0mg/L)的培养基对外植体进行预培养,以及在分化培养基中加入AgNO3(2￣6mg/L),可显著降低外植体褐化坏死的频率,提高了不定芽的发生频率及其再生能力;6BA(2.5mg/L)与NAA(0.1mg/L)配合使用有利于提高不定芽发生频率;再生的不定芽90%可长根。     

保护地番茄2.4-D药害症状识别与防治

番茄在冬春日光节能温室、早春大棚等保护地栽培生产过程中，为防止落花、落蕾，一般均要用2．4－D蘸花。如果蘸花不当，便会出现2．4－D药害。该药害不仅造成减产，最主要还造成尖顶果和畸形果，影响果实的商品价值。     

2-D数字阀用低惯量旋转电磁铁及其矩角特性研究

针对传统2-D数字阀用电-机械转换器转动惯量大、动态响应低的不足,研制了一种空心杯转子结构的低惯量旋转电磁铁.通过磁路解析讨论了电磁铁结构和运行参数对输出力矩的影响;基于Ansoft/Maxwell 3-D平台对其矩角特性进行了有限元模拟.试验结果和仿真结果基本一致,该低惯量旋转电磁铁具有较大的静力矩,其矩角特性近似于正弦波形,可用作高频2-D数字阀的电-机械转换器.     

基于三维激光扫描技术的粮食储量监测系统的设计与试验

为实现仓储粮储量在线实时监测,该研究开发了一种基于三维激光扫描技术的粮食储量在线监测系统。采用自主研制的倒置式粮仓专用型三维激光扫描仪对储粮进行扫描,通过上位机通讯、采集点云数据并控制扫描仪的工作过程,应用粮食体积计算软件实时计算储粮体积和数量,从而解决了仓储粮储量快速高精度监测的问题。使用该系统在中储粮某直属库进行系统验证试验,结果表明,测量得到的粮食体积满足最大误差不超过1%的技术指标,且经过多次试验检验,系统具有较好的稳定性、测量精度高、操作简便等优点,能够满足仓储粮储量监测的要求。该研究为实现仓储粮储量的快速实时在线监测提供了有效的方法。     

紫花苜蓿品种'德宝'不同外植体愈伤组织诱导研究

以紫花苜蓿'德宝'的下胚轴、叶片、子叶和叶柄为外植体,研究了不同浓度(1.5、2.0、2.5 mg/L)2,4-D对各外植体愈伤组织诱导的影响.结果表明:4种不同外植体的愈伤组织发生能力存在显著差异,下胚轴的愈伤组织诱导率最高,达98.5％,显著高于叶片(P＜0.05),其他各外植体之间差异不显著.不同浓度2,4-D下各外植体愈伤组织发生也存在显著差异,浓度1.5 mg/L和2.0 mg/L的2,4-D MS培养基对愈伤组织的诱导效果优于浓度2.5 mg/L的2,4-D MS培养基(P＜0.05),且有利于愈伤组织的胚性化.另外,2,4-D浓度在1.5～2.5 mg/L范围内,低浓度利于叶片和子叶愈伤组织发生,二者在1.5mg/L浓度下的愈伤率可达100％,显著高于2.5 mg/L浓度处理,而叶柄和下胚轴的愈伤率在各浓度下差异不显著.     

海滨木槿叶片蛋白质双向电泳体系的建立

为建立一套适合海滨木槿叶片蛋白质组学分析的双向电泳体系,以海滨木槿无性系叶片为材料,分别采用裂解液法、三氯乙酸一丙酮沉淀法、改良酚抽法分离纯化蛋白质,选用24empH3—10的IPG胶条,并对不同上样量、等电聚焦程序、平衡时间等电泳条件进行优化。结果表明：采用改良酚抽法提取蛋白质,上样量为每IPG胶条1000μg,总聚焦功率90kV·h,平衡时间15min,胶体考马斯亮蓝染色,可得到蛋白质点数目多、分辨率高的双向电泳图谱。重复性试验结果显示,该体系具有很好的稳定性及可重复性,可满足海滨木槿蛋白质组学研究的要求。     

2,4‐D 对大花月季硬枝扦插影响的研究

以红色大花月季为试材,研究了2,4‐D 对红色大花月季硬枝扦插影响.结果表明:红色大花月季扦插适宜的2,4‐D 浓度为25 mg?L‐1,浸泡的适宜时间为15 min ,此时愈伤组织和根系的生长量及成活率最高.     

18% 2，4 滴微乳剂防除薇甘菊技术的研究

在广东省深圳市薇甘菊为害严重的地区设置样地,用18%2,4-滴微乳剂不同浓度进行除治试验,结果表明:400～800倍液为有效浓度,1 000～1 200倍液为有效低浓度。不同生境样地防除试验结果表明:在山坡林地带可选用400～600倍液,在丘陵植被地带和果树周边地带可选用500～800倍液,在水塘、水库、水源周边地带以及菜地、花卉、药材和植物保护区周边地带可选用800倍液以下的有效低浓度。     

水环境中2,4-D光降解、O_3/H_2O_2降解的研究进展

综述了水环境中2,4-D光降解、O_3/H_2O_2降解的理论依据。主要对水溶液中2,4-D的降解所涉及的影响因素(包括辐射强度、吸光物质、pH、温度)对降解产生的影响作用和机理作了介绍,并进行了展望。     

Analysis of sinking death using video images of the swimming performance of Pacific Bluefin tuna (Thunnus orientalis) larvae

In this study, we aimed to clarify the mechanism of sinking death during the larval stage of Pacific Bluefin tuna Thunnus orientalis by investigating the effects of swimming performance on sinking death, using a behavioral approach. Swimming performance was examined 3–9 days after hatching (DAH) under day and night light conditions in cuboid experimental tanks. Swimming behavior variables such as swimming speed and swimming angle were measured under both light conditions. Larvae in the daytime experiment and larvae with inflated swim bladders at night were distributed on the surface layer of the water column. In contrast, larvae with uninflated swim bladders at night were frequently observed swimming vertically or sinking to the bottom of the tank. Larvae with inflated swim bladders at night were always distributed beneath the surface until the next morning (survival rates were 100 %). However, larvae with uninflated swim bladders at night swam upward repeatedly and later sank to the bottom of the tank (survival rates were 60 % and 38 % at 5 and 9 DAH, respectively). Larvae with uninflated swim bladders were not always able to maintain their swimming depth by swimming until the next morning. Additionally, their swimming speed and vertical swimming frequency (ratio) depended on the illumination and swim bladder conditions. Our findings show that larvae with uninflated swim bladders at night were associated with a higher risk of sinking death. The swimming energy capacity of Pacific Bluefin tuna larvae, which indicates the total amount of the energy that enables individuals to swim throughout the night without feeding, was found to be linked to sinking death.