旱区覆膜滴灌棉田N 2 O排放对化肥减量有机替代的响应

覆膜滴灌条件下,采用静态箱-气相色谱法研究了不同施肥策略:CK(不施肥)、CF(N 300 kg/hm~2;P2O590 kg/hm~2;K2O 60 kg/hm~2)、60%CF+OF(普通有机肥6 000 kg/hm~2)、60%CF+BF(生物有机肥6 000 kg/hm~2)对棉田土壤N_2O排放的影响,旨在明确滴灌棉田连续不同施肥策略下土壤N_2O的排放特征。结果表明,棉花生育期N_2O排放通量表现为施肥处理大于不施肥处理,滴灌施肥后第3/4天N_2O排放通量顺序为CF60%CF+OF60%CF+BFCK,而滴灌后第7/8天N_2O排放通量则表现为有机肥处理高于化肥处理,滴灌施肥结束后表现与之相同;生育期的N_2O排放总量以100%化肥处理(CF)最高,与其相比,60%CF+OF和60%CF+BF处理分别降低3.75%和8.37%,N_2O排放系数则分别降低1.39%和73.8%;相关及通径分析均表明,与土壤NH+4-N相比,NO-3-N与N_2O排放的关系更密切。     

CO 2与土壤水分交互作用的番茄光合速率预测模型

为了实现不同土壤水分管理下的CO 2气肥精细控制,建立了番茄作物不同生长阶段的光合速率预测模型。实验设置了4个CO 2浓度与3个土壤水分条件的交互处理,利用无线传感器网络长期实时监测温室内环境信息,采用LI-6400XT型光合速率仪定时采集作物净光合速率信息;并用BP神经网络分别建立了番茄苗期、花期和果期的光合速率预测模型。预测模型的验证结果表明,对于苗期预测模型,预测值与实测值之间的决定系数 R 2为0.925;花期预测模型的决定系数 R 2为0.920,果期预测模型的决定系数 R 2为0.958;番茄各生长期的光合速率预测模型均具有较高的预测精度。在不同土壤水分条件下改变CO 2浓度,得到的CO 2浓度与光合速率预测曲线与实测值相近,可反映实际土壤水分管理下的CO 2浓度最优值,对指导不同土壤水分条件下CO 2气肥的精细调控具有重要意义。     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

绿藤长茄新品种“慈茄2号”选育初探

在慈溪市白沙路街道现代农业示范园区,以韩家路黑藤茄为母本,以天元长茄为父本,杂交而产生变异杂合体,再通过系统选育方法选育成了绿藤长茄新品种——慈茄2号。其表现中熟,定植到采收40 d左右,自开花到商品嫩果采收18 d左右,全生育期130 d;茎、叶、果皮均为绿色,果实长条形,呈弯曲状;长势旺盛,耐热、耐寒、耐涝,连续结果能力强,产量高,品质好。     

CO2加富及亏缺灌溉对菜豆中期与后期生长的影响

为了探明菜豆中期与后期生长对CO₂加富与亏缺灌溉的生理响应,试验设两个CO₂水平:正常大气浓度和倍增CO₂浓度,两个灌溉水平:自然灌溉12次和亏缺灌溉6次,研究CO₂加富及亏缺灌溉对菜豆生长的影响。结果表明,CO₂加富显著提高菜豆生长中期株高与干质量,净光合速率(Pn)与胞间CO₂浓度(Ci)分别显著降低15.48%、37.67%,气孔导度(Gs)显著提高95.83%,菜豆生长后期的叶绿素a/b(Chla/b)显著提高12.29%,水分利用效率(WUE)显著提高46.51%,Ci显著降低12.87%。CO₂加富显著提高亏缺灌溉下生长中期菜豆的株高与干质量,但显著降低根冠比,显著提高叶绿素含量以及Gs;CO₂加富下生长后期的核酮糖-1,5-二磷酸羧化酶(Rubisco)及果糖-1,6-二磷酸酶(FBPase)活性分别显著提高78.05%与88.69%,CO₂加富使亏缺灌溉下碳酸酐酶(CA)与FBPase活性分别显著提高83.73%与64.84%。综上所述,CO₂加富提高菜豆生长中期对亏缺灌溉的适应性,对生长后期影响作用减小。     

The Exploitation of Nematode-Responsive Plant Genes in Novel Nematode Control Methods

The molecular interactions between plants and sedentary nematodes are undergoing intense study, not only for reasons of fundamental research but also for the potential benefits to agriculture. The present technology allows the transformation of an increasing number of crop plants, providing new ways to introduce resistance against plant-parasitic nematodes. The ability of sedentary nematodes to induce specialized feeding sites in plant roots is one of the most fascinating aspects of this host–parasite interaction. Molecular approaches have been initiated to identify and characterize plant genes altered in expression after infection by sedentary nematodes. The results obtained indicate that many genes indeed become up-regulated upon nematode infection. Surprisingly, several so-called constitutive promoters that are normally used to achieve high expression in plant cells are completely ‘silenced’ in the feeding sites within days after nematode infection. Generally, there are two options available for the genetic engineering of nematode resistance: the synthesis of anti-nematode proteins or the localized production of a cytotoxic protein that interferes with the development of feeding cells. Nematode-induced promoters are very useful for the production by plants of sufficiently high levels of anti-nematode proteins at feeding sites. Alternatively, interfering with feeding-cell development is somewhat similar to the hypersensitive response evoked by nematodes in a naturally resistant plant. Here, destruction of specific plant cells can be achieved by the localized expression of a cytotoxin such as barnase, a potent ribonuclease. This approach, however, calls for a highly specific ‘non-leaky’ promoter, which is active only in the feeding cells. Another possibility is to use a two-component system, where the leakiness of the promoter in other tissues is counterbalanced by the constitutive expression of a neutralizing gene.     

Synthesis and Anthelmintic Activity of 7-Hydroxy- 5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thiocarboxanilides

7-Hydroxy-5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thio-carboxanilides represent a novel series of anthelmintic compounds, with broad-spectrum activity against important parasitic nematodes in sheep and dogs. In particular, an improved efficacy against Trichostrongylus colubriformis in sheep over the related 3-carbamoyl-4-hydroxycoumarins has been noted. New synthetic routes to the key intermediate, 7-hydroxythieno[3,2-b]pyran-5-one, have been developed.     

Binding Sites for Ca2+-Channel Effectors and Ryanodine in Periplaneta americana—Possible Targets for New Insecticides

The calcium channel and the ‘calcium release channel’ of muscle membrane of the cockroach Periplaneta americana have been characterized. Biological assays with calcium channel blockers and ryanodine on different insects and acari revealed pronounced insecticidal effects with ryanodine, but not with calcium channel blockers, at concentrations between 0·1 and 300 μg ml⁻¹. Skeletal muscle membranes derived either from the tubular network or from the sarcoplasmatic reticulum of P. americana were characterized with respect to the binding of the dihydropyridine (DHP) [³H]isradipine (PN 200-110), the phenyl-alkylamine [³H]verapamil and the alkaloid [³H]ryanodine. Preliminary binding studies with the benzothiazepine [³H]diltiazem suggest a low-affinity binding site with a IC₅₀ value of 3·3 μM . All binding sites tested were sensitive to treatment with proteinase K. Optimal conditions for binding of the radioligand ryanodine revealed the highest specific binding at pH 8 and at calcium chloride concentrations between 100 and 500 μM . EGTA at 10 μM abolished 95% of the ryanodine binding. Binding studies with calcium channel binding sites revealed a pronounced effect of low Ca²⁺ concentrations on specific isradipine binding, whereas verapamil and diltiazem binding were only reduced by the presence of 200 μM EGTA. With respect to high Ca²⁺ concentrations, specific binding of diltiazem, isradipine and verapamil was reduced by 73, 40 and 20%, respectively, at 5 mM Ca²⁺. Radioligand binding experiments showed high-affinity binding sites for ryanodine and isradipine. K_D values of 0·95 nM (B_max=550 fmol mg⁻¹ protein) and 0·75 nM (B_max=213 fmol mg⁻¹ protein) were determined respectively. A lower-affinity binding site was identified in binding studies with verapamil (K_D=7·4 nM and B_max=27 fmol mg⁻¹ protein). [³H]isradipine displacement studies with several dihydropyridines revealed the following ranking of affinity: nitrendipine>isradipine>Bay K8664≪nicardipine. Displacement of [³H]verapamil binding by effectors of the phenylalkylamine binding site showed that bepridil and S(-)verapamil had the highest affinities of the compounds tested followed by (±)verapamil, nor-methylverapamil and R(+)verapamil.     

基于IPV6协议重构校园网的研究

为适应我国对第二代教育与科研网的建设,对互联网的IPV6协议进行了深入探讨,并在分析校园网的实际需求基础上提出了相应的解决方案,为校园网的重构提出了指导性的方法。