Wood, nematodes, and the nematode-trapping fungus Arthrobotrys oligospora

Researchers have proposed that Arthrobotrys oligospora and related fungi trap soil nematodes to obtain nitrogen and thereby compete saprophytically for carbon and energy in nitrogen-poor environments, including litter and wood. The current study tested two hypotheses concerning this model. The first was that wood decomposition would be enhanced if both large numbers of nematodes (a potential nitrogen supply) and A. oligospora (a cellulolytic organism that can use that N supply) were present. The second was that A. oligospora trapping activity would increase if large numbers of nematodes were added to soil containing abundant carbon (a wood dowel or chip). Although the first hypothesis was supported by an in vitro experiment on agar (A. oligospora degraded much more wood when nematodes were present), neither hypothesis was supported by an experiment in vials containing field soil. In soil, wood decomposition was unaffected by the addition of A. oligospora or large numbers of nematodes. Whereas A. oligospora trapped virtually all nematodes added to agar cultures, it trapped few or no nematodes added to soil. Given that the fungal isolate was obtained from the same soil and that the fungus increased to large numbers (>1×10³ propagules g⁻¹ soil), the failure of A. oligospora to trap nematodes in soil is difficult to explain. Soil nitrate levels, however, were high (71 mg kg⁻¹), and it is possible that with lower nitrate levels, trapping in soil might be stimulated by wood and nematodes.     

Seed Yield and Yield Contributing Characters as Influenced by N Supply in Rapeseed-mustard

Brasisca Juncea , cv. Pusa Bold, and B. campestris , cv. Pusa Kalyani, were raised under field conditions with varying levels of N supply from 0–120 kg ha^-1. The production profile of branches and pods thereon was measured, per unit area basis, throughout the crop ontogeny. At maturity, data on the yield contributing characters, viz. pod dry weight, pod number, seed number per pod, 1000 seed weight, seed wall ratio and seed yield in different order branches, was recorded.
The branching pattern and the number of pods produced on different order branches, in the two species, was favourably modified by the increasing levels of N supply. Primary and secondary branches contributed to the seed yield to an extent of 80 % of the total yield. Nitrogen treatment had no significant effect on 1000 seed weight. B. juncea exhibited significantly higher yield over B. campestris. N supply up to 120 kg ha ^-1 linearly increased seed yield in both the species. However, it exerted a negative effect too partitioning of assimilates from pod wall to seed. The study indicated that rapeseed-mustard, grown under short winter-season environment with adequate soil moisture, has the potential for higher N-fertilizer optima exceeding 120 kg ha ^-1.     

The indirect immunofluorescence assay using cardiac tissue from chickens, quails and ducks for identification of influenza A virus during an outbreak of highly pathogenic avian influenza virus (H5N1): a rapid and simple screening tool for limited resource settings

Here we describe the diagnostic utility of the indirect immunofluorescence assay (IFA) during a recent outbreak of highly pathogenic avian influenza (HPAI) subtype H5N1 virus in southern Thailand and demonstrate the usefulness of the cardiac tissue from infected chickens, quail, and ducks for diagnosis. The most reliable sample for IFA diagnosis of influenza A virus was cardiac tissue (83.0%; 44/53) which when divided by species (chicken, quail and duck cardiac tissues) gave respective positivity rates of 88% (22/25), 88.9% (16/18) and 60.0% (6/10). Cardiac tissue also gave the highest IFA intensity for the three species. We believe that the IFA method has wide applicability in developing countries or remote settings where clinically similar avian diseases with high morbidity and mortality such as Newcastle disease and fowl cholera are common and could be rapidly excluded thereby conserving valuable reference laboratory capacity for true HPAI outbreaks.     

菜地土壤N_2O排放及其氮素反硝化损失

采用培养试验方法,对南京郊区 3对菜地土和水稻土的 N2O排放和氮素反硝化损失进行了研究.不加乙炔培养测定土壤 N2O排放,加乙炔( 10% V/V)培养测定反硝化损失.菜地土为相同类型水稻土改种为蔬菜约 20年的土壤.结果表明,在培养 0～ 1 d期间 ,菜地土本身 N2O排放通量 (5.15～ 218.37 ng N· g-1soil· h-1)均高于相同类型的水稻土 (2.50～ 3.94 ng N· g-1soil· h-1). 3对供试土壤中, 2个菜地土培养 21 d排放的 N2O总量与反硝化损失总量均显著高于相同类型的水稻土( P<0.05). 3对供试土壤施尿素后反硝化损失均未显著增加.施肥和不施肥处理,土壤 N2O排放累积量和反硝化损失累积量随时间 t的变化均符合修正的 Elovich方程 y=bln(t)+ a.     

湖泊沉积物对N和P的吸附特性及影响因素研究

以太湖沉积物为悬浮颗粒物(SPM)材料，进行了SPM对磷酸盐(PO4-P)、氨氮(NH4-N)、硝酸盐氮(NO3-N)的吸附特性及其影响因素的试验研究。结果显示，SPM对PO4-P和NH4-N的吸附分别在5h和2h达到稳态，之后基本处于一种动态平衡。SPM对NO3-N的吸附量很小甚至出现负吸附。pH对SPM吸磷作用影响显著，随着pH值的上升，吸附量下降。盐度、[NO3^-]和[NHg]对PO4-P的吸附有抑制作用，而[Ca^2 ]对PO4-P的吸附影响分为2部分，在低浓度下抑制，在高浓度下有促进；[Ca^2 ]和盐度对NH4-N的吸附都有抑制作用。     

土壤腐殖质氮结构的X-光电能谱研究

X－ray photoelectron spectroscopy(XPS) was applied to examine the N structures of soil humic substances and some of their analogues.It was found that for soil humic substances XPS method gave similar results as those obtained by ^15N CPMAS NMR (cross-polarization magic-angle spinning nuclear magnetic resonance) method.70%-86% of total N in soil humic substances was in the form of amide,and 6%-13% was presented as ammes,with the remaining part as heterocyclic N.There was no difference in the distribution of the forms of N between the humic substances from soils formed over hundreds or thousands of years and the newly formed ones.For fulvic acid from weathered coal and benzoquinone-(NH4)2SO4 polymer the XPS results deviated significantly from the ^15N CPMAS NMR data.     

用^137Cs法探讨苏南坡地的土壤侵蚀

与黄土和红壤地区相比 ,学术界对苏南丘陵区土壤侵蚀的关注要少得多 ,也许是这里的土壤侵蚀的绝对数量不大 ,然而 ,苏南地区的土壤侵蚀引起的生态影响却不容忽视 ,如因土壤侵蚀造成的养分流失对水体的影响及土层减薄都远超过黄土区。为了探讨苏南土壤侵蚀的生态影响 ,首先是要了解土壤侵蚀的状况 ,在这方面 13 7Cs示踪法是一种非常有用的工具 ,可是要使用此法 ,必须要解决两个问题 ,一是确定研究区的背景值 ,二是建立估算土壤侵蚀的模型。经过较长时间的研究 ,确定苏南地区的 13 7Cs背景值为 2 2 0 0 Bq/m2 ;并根据基本物理含义 ,建立了耕地与非耕地的土壤侵蚀模型 ,用这 2个模型并借用其他学者提供的土壤沉积模型 ,对宜兴竹园及茶园两个坡地的土壤侵蚀与沉积作了估算。在此基础上 ,又尝试着简单探讨了土壤侵蚀引起的土层及养分流失的生态影响     

基于土壤侵蚀定位土芯元素示踪法和GIS技术红壤小流域坡地土壤肥力的综合评价

利用定位土芯稀土元素(Eu)示踪法及中子活化技术,对蒋家塘小流域坡地土壤侵蚀空间分 布规律进行了研究,并利用模糊数学的方法,建立了影响蒋家塘小流域侵蚀土壤肥力因素单因子评价模型,利用层次分析法确定各评价因子的权重,并用加权法得到 每个评价单元的土壤肥力综合指数,根据其数值的大小,将土壤肥力分为高(>0.45)、中( 0.25～0.45)和低(<0.25)3级.借助地理信息系统软件ARCVIEW3.1对蒋家塘小流域坡地土壤 侵蚀状况和土壤肥力状况进行了分析,结果表明地形部位较高,坡度较大,植被较少或裸 露的坡顶及上坡部位,土壤侵蚀量较大,侵蚀程度严重;而在地形部位较缓,植被覆盖度较 大的坡段土壤侵蚀量较小,侵蚀较轻.植被覆盖度较小,顺坡种植的坡段,土壤侵蚀量较大 ,土壤侵蚀严重;而植被覆盖度较大,等高梯田种植的坡段土壤侵蚀量较小,土壤侵蚀较轻 ;下坡部位土壤往往发生沉积.在整个小流域中,侵蚀程度严重(>100 t/hm2*a)的坡地面积比例为15.67%,侵蚀程度中等(10～100 t/hm2*a)的坡地比例为22.13%,侵蚀程度较轻(1～10 t/hm2*a)坡地比例为5.60%,无侵蚀(<1 t/hm2*a)的坡地比例为34 .23%.土壤肥力较高的坡地面积比例为22.00%,中等的为34.75%,低下的为20.85%.蒋家塘 小流域坡地土壤肥力水平多数处于中下等水平,占总流域面积的55.60%,占坡地面积71 .62%. 水土流失是造成土壤肥力降低,土壤贫瘠化的一个最重要的原因,侵蚀严重的地方,土壤肥 力低下,侵蚀较轻或沉积的地方,土壤肥力较高,土壤侵蚀模数与土壤肥力指数之间的相关指数为0.801(n=33),达极显著水平.     

氮磷肥对黑土玉米农田生态系统土壤微生物量碳、氮的影响

通过田间氮磷肥配施试验研究了氮磷配施对黑土玉米农田生态系统玉米不同生育时期微生物量碳、氮的影响。微生物量随玉米不同生育期的动态变化表明,氮磷肥对微生物量碳和微生物量氮的动态影响并不同步,微生物量碳和微生物量氮变化最显著的时期均是授粉期,但此时微生物量碳是最低的谷值,而微生物量氮是最高的峰值。不同氮磷配比对微生物量碳影响的回归分析表明,氮肥是影响微生物量碳的主导因素,无论是适量施用还是过量施用都是氮肥对微生物量碳的影响较大。不同氮磷配比对微生物量氮影响的回归分析表明,过量氮肥的施用减少了土壤微生物量氮的含量。磷肥无论高量和低量均能增加微生物量氮的含量,但随着施用量的增加对微生物量氮的正效应减小。氮磷配合施用可增加土壤的微生物量氮,由此可见无论单施氮肥还是单施磷肥,过量施用对微生物量氮的增加都是不利的,只有氮磷配合施用才是增加土壤微生物量氮的有效途径。     

不同生育时期根际土壤渍水逆境对冬小麦N、P、K素营养的影响

运用盆栽模拟土壤渍水逆境试验，研究不同生育时期根际土壤渍水逆境对不同小麦品种N、P、K素吸收、运转和分配的影响。结果表明，根际土壤渍水逆境对不同小麦品种N、P、K素吸收的影响有异;不同生育时期根际土壤渍水逆境显著影响根系对N、P素的吸收、运转与分配，以孕穗期渍水逆境影响最大，其次为灌浆期和拔节期，而不同生育时期根际土壤渍水逆境对K素的吸收影响较小。孕穗期以前浈水逆境主要影响小麦根系对N，P，K案的吸收．对N、P、K素在小麦体内的运输和分配影响较小；灌浆期渍水逆境不仅影响根系对N、P、K素的吸收．同时也影响N、P素在地上部各器官中的运转和分配，但对K素在小麦体内的运转和分配影响较小。因此，基肥中施足P肥和K肥，拔节孕穗期重施速效N肥，灌浆期叶面喷施KH2PO4对于培育壮秆大穗，减轻小麦溃害，提高受渍小麦籽粒产量具有非常重要的实际意义。