全文获取类型
收费全文 | 18393篇 |
免费 | 1049篇 |
国内免费 | 1710篇 |
专业分类
林业 | 1062篇 |
农学 | 1714篇 |
基础科学 | 218篇 |
1013篇 | |
综合类 | 6221篇 |
农作物 | 1496篇 |
水产渔业 | 695篇 |
畜牧兽医 | 4551篇 |
园艺 | 3045篇 |
植物保护 | 1137篇 |
出版年
2024年 | 79篇 |
2023年 | 304篇 |
2022年 | 568篇 |
2021年 | 680篇 |
2020年 | 711篇 |
2019年 | 869篇 |
2018年 | 629篇 |
2017年 | 798篇 |
2016年 | 1107篇 |
2015年 | 1022篇 |
2014年 | 1111篇 |
2013年 | 1072篇 |
2012年 | 1457篇 |
2011年 | 1532篇 |
2010年 | 1233篇 |
2009年 | 1217篇 |
2008年 | 1087篇 |
2007年 | 1180篇 |
2006年 | 908篇 |
2005年 | 769篇 |
2004年 | 527篇 |
2003年 | 453篇 |
2002年 | 300篇 |
2001年 | 266篇 |
2000年 | 227篇 |
1999年 | 178篇 |
1998年 | 129篇 |
1997年 | 107篇 |
1996年 | 104篇 |
1995年 | 64篇 |
1994年 | 64篇 |
1993年 | 71篇 |
1992年 | 49篇 |
1991年 | 36篇 |
1990年 | 30篇 |
1989年 | 36篇 |
1988年 | 25篇 |
1987年 | 22篇 |
1986年 | 25篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 19篇 |
1980年 | 10篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1962年 | 7篇 |
1956年 | 14篇 |
1955年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 390 毫秒
61.
62.
63.
XU Rui ZHANG Yun ZHANG Mei GE Zhi-ming CHEN Wen-qiang LI Xiu-chang CAI Heng ZHU Hui 《园艺学报》2003,19(5):642-644
AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp/dtmax) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp/dtmax) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp/dtmax and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp/dtmax and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction. 相似文献
64.
AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO2-/NO3- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO2-/NO3- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway. 相似文献
65.
66.
AIM: To study whether Sca-1+ cells from fetal liver can be induced to differentiate into neuronal cells in vitro. METHODS:Sca-1+cells from 14 5-days-old murine fetal liver were isolated with a magnetic cell sorting kit, and were cultured in Dulbecco s modif ied Eagle s medium(DMEM)/F12 supplemented with 10%fetal bovine serum(FBS), and passaged at a rat io of 1 3 when cells reached more than 80%confluence.The 5 passage cells were induced by 10-3mol/Lβ-mercaptoethanol(β-ME)and 5×10-7 mol/L all-trans-retinoic acid(RA)for 24 hours, and then incubated in serum-free medium for 5 hours to 5 days.The characteristics of treated cel s were assayed by immunocytochemistry staining analysis at 5 hours, or 5 days.RESULTS: Cells treated with β-ME and RA exhibited neuronal phenotype and expressed neuron-specific protein such as neuron-specific nuclear protein (NeuN), neuronfilament-M, and neuron-specific tubulin-1 (TuJ-1) but not tau, MAP-2, or the astrocyte-specific marker glial fibrillary acidic protein (GFAP).CONCLUSION: Sca-1+ cells from fetal liver, of which most are regarded as hematopoietic stem cells, could differentiate into early immature neuronal cells in vitro. These findings suggest that Sca-1+ cells from fetal liver may be an alternative source in cell therapy and gene therapy of neural dysfunction. 相似文献
67.
AIM:To explore transdifferentiation potential of Sca-1+ cells from murine fetal liver. METHODS:2×103 of Sca-1+ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from 60 Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA+/CD45-F4/80- and NueN+/CD45-F4/80-, respectively. CONCLUSION:The evidence is provided for Sca-1+ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells. 相似文献
68.
AIM:To investigate therapeutic effects of recombinant human growth hormone(rhGH) on rat sepsis and its possible mechanisms.METHODS:Mean arterial pressure (MAP), levels of plasma TNFα, IL-1β and endotoxin, leukocyte count and survival rate within 1 week were determined after E. coli injection among control group, sepsis group and sepsis+rhGH group.RESULTS:(1)rhGH diminished the decrease of MAP, reduced plasma endotoxin and TNFα levels and increased neutrophil ratio in total leukocytes in sepsis rat. rhGH increased survival rate within 1 week on sepsis rat. (2)No changes were found in IL-1β level among the three groups.CONCLUSION:rhGH showed desirable beneficial effects on rat sepsis, which may attribute to: improving circulatory function;maintaining intestinal mucosa barrier, attenuating bacteria/endotoxin translocation and inhibiting the production and release of TNFα. 相似文献
69.
AIM:To explore the serum levels of certain adhesion molecules and its significance in acute coronary syndrome(ACS). METHODS:The subjects included 40 patients with acute myocardial infarction(AMI) and 40 patients with unstable angina pectoris (UAP). Among the 80 patients, 60 patients accepted a follow-up 4 months. At the same time we selected 40 controls from people who attended a routine health check in the university. Serum levels of E-selectin, sICAM-1, sVCAM-1 were measured by ELISA.RESULTS:Serum levels of E-selectin, sICAM-1, sVCAM-1 were significantly higher in the ACS group(AMI or UAP) than in the control group. Four months later, the levels of E-selectin, sICAM-1 became significantly lower in the follow-up group than in the ACS group, while sVCAM-1 showed no significant difference. CONCLUSION:Serum levels of E-selectin, sICAM-1 may have certain diagnostic value for ACS, and can be a useful marker reflecting the stability of the disease. 相似文献
70.