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141.
OBJECTIVE: The aim of this study was to characterize the clinical and morphologic features of neuronal ceroid lipofuscinosis (NCL) in the Polish Owczarek Nizinny (PON) breed of dog. ANIMALS: Nine Swedish PON dogs of both sexes were included in the study. PROCEDURE: All dogs underwent a detailed clinical evaluation, with emphasis on ophthalmic exams. Histopathology and electron microscopy were performed on the eyes, brain and various internal organs. Immunohistochemical staining for detection of sphingolipid activator proteins (SAPs) and mitochondrial ATP synthase (SCMAS) was performed on the eyes and brain. RESULTS: The dogs showed behavioral abnormalities, motor disturbances and visual impairment or blindness. Pupillary responses were abnormal while fundus changes varied from normal to severe retinal atrophy. Electroretinography (ERG) showed variable changes, from slight alterations in the process of dark adaptation to severely reduced or nonrecordable ERG a- and b-wave amplitudes. Histopathology revealed intracytoplasmic storage bodies within neurons of the brain and in retinal cells, especially the retinal pigment epithelium (RPE). Round to oval granular type of inclusion bodies, known as granular osmiophilic dense deposits (GRODS), were found in neuronal cells in the brain and in the retina. Immunohistochemistry identified the storage material in the brain and retina as consisting of SAPs. CONCLUSION: The presently described NCL disease in PON dogs shows similarities to previously recorded cases in the Miniature Schnauzer. The closest human equivalent to this disease is infantile NCL (CLN1), in which the major stored proteins are SAPs and the ultrastructure of the inclusion bodies of neuronal cells is granular. 相似文献
142.
This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved. 相似文献
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甘肃省“121”雨水集流工程经济 总被引:1,自引:0,他引:1
李莉 《中国农村水利水电》2007,(9):50-52
甘肃中东部地区地处黄土高原,气候干旱,水资源缺乏,农村人畜饮水困难。“121”雨水集流工程的实施,有效地解决了这些地区的人畜饮水困难。为了客观评价“121”雨水集流工程的经济效益和作用,利用建设项目后评价理论与方法,对工程的经济效益进行了科学评价,国民经济评价结果可行,其结果可用于指导全省雨水利用工程的规划、设计,加强雨水利用工作的决策、管理。 相似文献
145.
以成年油木奈树为材料,对其初始坐果进行了研究。结果表明,盛花后2周内,油木奈出现大量的落花落果;幼果(子房)低水平的GA1 3与油木奈的落花落果高峰关系密切,而内源细胞分裂素ZRs和[diH]ZRs以及ABA与幼果(子房)的生长呈显著的正相关;初始坐果期,油木奈的雌配子体发育缓慢,且有相当数量的胚囊中途退化、败育,无法正常受精,从而导致其大量的落花落果。文中就油木奈的初始坐果机制进行了讨论。 相似文献
146.
通过对蒙古沙棘亚种优良品种与中国沙棘亚种优良类型F1代雄株的变异特性分析,建立了选择优 良单株的标准,为叶用型沙棘选择育种提供了科学依据。 相似文献
147.
AIM To analyze the regulatory effect of quercetin (QUE) on PTEN-induced putative kinase 1 (PINK1)/parkin mitochondrial autophagy pathway, and to explore the mechanism of quercetin in relieving cerebral ischemia/reperfusion (I/R) injury. METHODS Sixty SD male rats were randomly divided into sham operation group, model group (I/R group), QUE group,3-methyladenine (3-MA) group and QUE+3-MA group. Administration started in each group 3 days before modeling, once a day, at 30 min after the last administration,except sham group, the other groups used 4-vessel blockage method to establish the whole brain I/R model. On the day after modeling, the neural function was evaluated by neuropathy disability score (NDS). The volume of cerebral infarction was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The morphological changes of mitochondria in hippocampus were observed by transmission electron microscopy. The contents of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in hippocampus were measured by ELISA. The activity of superoxide dismutase (SOD) and contents of malondialdehyde (MDA) in hippocampus were detected by xanthine oxidase method, thiobarbituric acid condensation method. Western blot was used to detect the proteinex pression of PINK1, parkin and LC3-II in brain tissue. RESULTS Compared with sham group, the hippocampus of the rats in I/R group and QUE+3-MA group showed swelling of mitochondria, destruction or disappearance of internal crista and other pathological damage,also the volume of cerebral infarction, the contents of IL-6, TNF-α and MDA, the protein expression levels of PINK1, parkin and LC3-II were increased (P <0.05), while NDS score and activity of SOD were decreased (P <0.05). Compared with I/R group and QUE+3-MA group, the pathological damage degree of hippocampus in QUE group was reduced, the volume of cerebral infarction, the contents of IL-6, TNF-α and MDA were decreased (P <0.05), the proteinexpression levels of PINK1, parkin and LC3-II, and NDS score and activity of SOD were increased (P <0.05).The above indexes in 3-MA group were opposite to QUE group. No significant difference in the above indexes between I/R group and QUE+3-MA group was observed (P >0.05). CONCLUSION Quercetin activates mitochondrial autophagy and reduces cerebral I/R by regulating the expression of PINK1/parkin pathway proteins. 相似文献
148.
149.
HUANG Zeng-yan HUANG Man-ping PENG Chun-li LIU Yong-yuan LI Chun-yan LIANG Dong-hui 《园艺学报》2021,37(1):34-40
AIM To observe effects of emotional stimulation on expression of stromal cell-derived factor-1(SDF-1) and CXC chemokine receptor 4 (CXCR4) in plasma, platelets, aortas, hippocampus and bone marrow of apolipoprotein E gene knockout (ApoE -/-) mice, and to reveal the possible mechanism of the aggravated atherosclerotic plaque vulnerability by emotional stimulation. METHODS Thirty 8-week-old male ApoE -/- mice were randomly divided into normal control group, high fat group, and emotional stimulation group. Ten 8-week-old inbred C57BL/6J mice served as blank control group. After 12 weeks of intervention, the serum levels of SDF-1 and CXCR4 were investigated by ELISA. The protein levels of SDF-1 and CXCR4 in platelets, aortas, hippocampus and bone marrow were determined by Western blot. The pathological damage of aortas was observed by oil red O staining. RESULTS Compared with blank control group, normal control group and high fat group, the mice subjected to emotional stimulation showed more serious atherosclerosis in aortas detected by oil red O staining, and increased levels of SDF-1 and CXCR4 in the plasma and aortas were also observed (P <0.05). The results of Western blot showed that the protein levels of SDF-1 and CXCR4 in platelets, aortas and hippocampus were increased in the mice subjected emotional stimulation, but the expression of SDF-1 and CXCR4 in the bone marrow was decreased (P <0.05). CONCLUSION Imbalance of SDF-1/CXCR4 may be the key target by which emotional stimulation accelerates the progression of atherosclerosis. 相似文献
150.