Study on Liver-protective Effect of Chinese Herbal Compound Probiotics on Acute Liver Injury Layers

This experiment was conducted to investigate the liver-protective effect of Chinese herbal compound probiotics (CHCP) on acute liver injury layers.One hundred and eight 1 day old hens were divided into 4 groups with 3 replicates in each group and 9 layers per replicate in trial 1.The layers in model groups Ⅰ to Ⅲ were gavaged with 10% (V/V) soybean oil solution of carbon tetrachloride (SCCl₄) according to 1,2 and 4 mL/(kg·BW) at 14,28 and 35 d,respectively.The layers in control group were gavaged with 2 mL/(kg·BW) soybean oil.In trial 2,sixty 1 d layers were divided into 5 groups:Control group (soybean oil),model control group(SCCl₄)and low-dose,middle-dose and high-dose CHCP group (SCCl₄+1‰ CHCP,SCCl₄+2‰ CHCP and SCCl₄+4‰ CHCP respectively).CHCP were used by drinking water since 7 days.SCCl₄ were gavaged according to 2 mL/(kg·BW) at 14 and 28 d.The results showed as follows:The model of layers liver damage could be built by intragastric administration of 2 mL/(kg·BW) 10%(V/V) SCCl₄ at 14,28 d respectively,with the signs of hepatic steatosis,severe vacuolar degeneration,nuclear condensation and necrosis.Compared to the model control group,the serum AST levels in low,medium and high dose CHCP groups were decreased by 4.35% (P > 0.05),7.57% (P > 0.05) and 9.79% (P < 0.05),the serum ALT levels in medium and high dose CHCP groups were decreased by 34.92% (P < 0.01),36.51% (P < 0.01),the serum total bilirubin content in medium and high dose CHCP groups were decreased by 25.49% (P < 0.01),27.45% (P < 0.01).The liver cell congestion was reduced to varying degrees in different dose CHCP groups,and the liver cell had no vacuoles,arranged in neat rows,abundant cytoplasm and uniform in different dose CHCP groups.In conclusion,2‰,4‰ CHCP could reduce hepatocyte necrosis,decrease the serum activities of ALT,AST and total bilirubin levels,and had protective effect on hepatic injury induced by SCCl₄.     

高铜日粮对肉鸡肝脏Trx2蛋白表达的影响

将200羽1日龄Cobb商品代肉鸡随机分为4组,各组日粮中铜含量分别为:对照组(Ⅰ组)11 mg/kg、试验Ⅱ组110 mg/kg、试验Ⅲ组330 mg/kg、试验Ⅳ组550 mg/kg。饲养至10、30、50 d时每组各取5只鸡用于采集肝脏样品,提取肝脏总RNA用RT-PCR法测定Trx2基因m RNA在肝脏中的表达量,用蛋白质印迹法检测Trx2蛋白表达量。结果显示:日粮铜含量为11 mg/kg、110 mg/kg和330 mg/kg时,随着试验时间的延长,肝脏Trx2基因表达升高,但差异不显著(P0.05),而铜含量为550 mg/kg时,Trx2基因表达量降低且50 d时差异显著(P0.05);随着试验时间的延长,各试验组的Trx2蛋白表达量均升高,且330 mg/kg和550 mg/kg组在50 d时差异显著(P0.05)。试验表明,饲喂高铜日粮可导致线粒体型硫氧还蛋白2(Trx2)mRNA在肝脏中的表达量先升高后降低,蛋白表达量升高。     

猪喘气病的鉴别诊断和防治

猪喘气病也叫猪地方流行性肺炎(SEP)或猪支原体肺炎(MPS),是由猪肺炎支原体(Mhp)引起的一种传染性肺炎。不同品种、年龄、性别的猪均能感染,以哺乳仔猪和幼猪最易感,其次是妊娠后期的母猪,育肥猪和成年猪多呈慢性和隐性感染。主要症状为咳嗽和气喘,病变特征是两肺的尖叶、心叶、中间叶和膈叶呈对称性肉样或虾肉样实变。近年来发病有上升趋势,主要原因是随着集约化饲养的增多,群体之间传播概率增大。尽管此病诊断治疗不困难,但是此病易与猪肺疫流感等病混淆造成误诊,临床治疗不及时往往造成很大损失。本文主要讨论了猪气喘病的临床症状及病理剖检变化、鉴别诊断,并提出有效防治措施。     

重金属Cu~(2+)对四角蛤蜊的急性毒性及肝脏组织结构的影响

在水温(12±0.5)℃、盐度27‰的环境下,研究了重金属Cu~(2+)对四角蛤蜊的急性毒性效应和肝脏组织结构的影响。结果表明,Cu~(2+)对四角蛤蜊24、48、96hLC50分别为13.4、7.34和4.32mg/L;安全浓度分别为0.13、0.07和0.04mg/L。通过对Cu~(2+)暴露在不同浓度96h的四角蛤蜊肝脏组织切片观察发现,Cu~(2+)对四角蛤蜊的肝脏组织结构有明显影响,肝脏的损伤程度随着Cu~(2+)浓度的增加而加深,2.59mg/L为四角蛤蜊肝脏解毒的阈值。     

鸡心包积液综合征的防治

2010年11月,吉林省某养鸡户肉鸡突然出现死亡。病死鸡解剖后发现心包积液非常严重,肝脏肿大坏死。经过初步诊断确定为鸡心包积液综合征,该病又称鸡心包积水-肝炎综合征(HHS),最早于1987年发生在巴基斯坦卡拉奇的安格拉地区,因此又称"安格拉病",在一年内迅速     

新生羔羊肝脓肿的诊断与治疗

新生羔羊肝脓肿是由致病菌经脐带感染而引起的一种疾病，多发生于7日龄后羔羊，病理变化以肝脏形成豌豆大脓肿为特征。本病发病急，病程短，若诊治延迟失误，常引起病羔死亡。有关新生羔羊肝脓肿的报道鲜见，笔者在长期的羔羊疾病诊疗中，曾收治过51例，治愈40例，治愈率为78％，现将诊治情况介绍如下。     

护好手足不皲裂

<正>冬季,容易发生手足皲裂,且一旦发生治愈较慢,因此要做好预防,加以注意。首先,要注意保暖,外出时一定要戴好手套,鞋一定要保暖。其次,皮肤比较干燥的人,可以用一些油脂性的护肤品涂抹手足。第三,尽量少接触凉水,减少冷水洗手的次数,少用碱性强的肥皂、药皂洗手,容易发生手足皲裂的,洗完手脚后涂抹油脂类护肤品。另外,在饮食上,适量摄入富含蛋白质的     

猪渐进性萎缩性鼻炎——猪场不容无视的问题

<正>猪渐进性萎缩性鼻炎(PAR)是一种遍布全球的传染性疾病。根据猪病兽医顾问Carlos Casanovas Granell的描述,该病可产生严重的经济影响,在很大程度上降低受感染动物的生长率。本文的目的是评估一个养殖场受到渐进性萎缩性鼻炎(PAR)感染所造成的额外费用,并确定繁育母猪通过疫苗接种措施预防的可行性。一、简介自从1830年起,人们已经认识到猪萎缩性鼻炎属于一种传染性疾病,并在全球内广泛地蔓延。本病主要是由产毒素多杀性巴氏杆菌和支气管败血波氏杆菌引起的,