公鸡精液和肛拭子样品禽白血病抗原检测对比研究

本研究对2014—2015年某地方品种5个品系12个批次共1 512只公鸡的精液和肛拭子样品进行了禽白血病p27抗原ELISA检测和对比分析,结果发现二者的一致性平均为83%左右。当精液和肛拭子抗原阳性率越高时,二者的一致性越差。经过两个世代的净化,无论是精液还是肛拭子p27抗原平均阳性率都下降了4个百分点左右。因此,需要采取精液结合肛拭子ELISA检测的方法,共同检测淘汰阳性个体。对公鸡进行单一检测方法难以满足净化需要。     

如何提高二元母猪的受胎率

<正>随着陕西镇坪县生猪产业的迅速发展,生猪品种也日趋良种化,特别是基础母猪,目前基本上为外二元母猪,相对于土种(本地品种)猪而言,在繁殖生理特征上有较大差别。主要表现为初情期推迟,发情特征轻微难以确定适宜的配种时间。生产上表现为配种不易受胎或情期受胎率低,产子数少等问题,影响了养猪的经济效益和     

提高母牛冻精配种繁殖能力的方法

要想提高母牛冷冻精液配种的繁殖率,达到多配、多怀、多产、多活应该抓好以下几个主要环节。1提高母牛受配率（1）提高母牛膘情,促进母年发情,从而提高母牛的受配率。     

影响猪人工授精效果的主要原因及改进措施

随着畜牧科学技术进步,生猪人工授精配种从二十世纪七十年代开始推广应用于我国养猪生产中,在养猪场和农村养猪户中已逐步被接受和推广应用,并且大部分效果明显,如情期受胎率高、产仔数多、母猪生殖道疾病少等。该技术的推广应用,对加速生猪良种推广和经济杂交改良步伐,推进我国生猪生产快速健康发展做出了巨大的贡献,但在当前广大农村应用生猪人工授精配种中还存在一些生产技术问题,在一定程度上影响了该技术     

四种猪精液稀释粉的比较试验

试验通过对比四种不同精液稀释粉对6头公猪精液稀释后保存效果,发现精液保存天数活力＞0.6)最长的是Modena 为8.67d,MⅢ为5.67 d,ART.NR为5.33d,朱哥宝为4.33d.Modena稀释粉显著好于MⅢ(P＜0.05),极显著好于其他二种稀释粉保存效果(P＜0.01)；MⅢ对精液的保存效果又显著好于ART.NR,朱哥宝二种稀释粉.ART.NR、朱哥宝二者对精液的保存效果没有显著差别.     

不同类型獭兔的饲养管理

1种公兔 要提供充足、优良的蛋白质日粮和补充块、茎饲料,则种公兔性欲旺盛,精液品质好,配种受胎率高。留种公兔从3月龄开始应实行每只兔1个笼,以防早配、滥配。严格控制初配月龄。在正常饲养管理条件下,青年公兔的初配月龄应控制在8~9月龄,     

热应激对种公犬精液品质的影响

热应激通常是指在环境温度超过动物的适温区上限的影响下,因体内产热与散热失衡而导致的一系列生理与行为机能上的负面表现。 一、热应激对公犬精液品质的影响 （一）热应激影响公犬精液品质的机理 热应激使公犬采食量显著下降,营养物质供应不足,体内酸碱平衡调节受到影响,机体组织细胞机能和新陈代谢发生变化。种公犬在高温环境下阴囊和睾丸组织的温度升高,睾丸机能减退。     

日粮中添加复方中草药对巴马香公猪精液品质及免疫功能的影响

试验选用巴马香公猪为研究对象,旨在研究复方中草药在提高公猪精液品质和免疫功能方面的作用。将12只巴马香公猪随机分为4组,每组3只,分别为对照组和3个给药组（低、中、高剂量给药组）。对照组仅饲喂基础日粮,低、中、高剂量组在基础日粮中分别添加1%、2%和3%的复方中草药。分别在给药后第0、7、14和21天采集精液,检测精液量、精子活力和精子畸形率|空腹前腔静脉采血,测定IgA、IgG和IgM含量。结果表明,日粮中添加复方中草药可提高巴马香公猪精液品质和免疫功能。其中,以添加中剂量复方中草药效果最好。 [关键词]复方中草药|巴马香公猪|精液品质|免疫功能     

Effect of Se and VE Supplement on Semen Quality,Antioxidant Enzyme Activities and Heat Shock Protein Expression of Goat in High Temperature Season

This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality, antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4 groups, fed with basal diet(control group), basal diet+0.5 mg/kg Se(Se group), basal diet+100 mg/kg VE(VE group), and basal diet+0.5 mg/kg Se+100 mg/kg VE(Se+VE group), respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality, antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group, the ejaculate volume was not significantly affected by Se or VE supplement(P>0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P<0.05), and the abnormal rate was decreased extremely significantly(P<0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P<0.01), SOD(P<0.05), CAT(P<0.05) and T-AOC(P<0.01), and lower MDA concentration in seminal plasma(P<0.05).The relative expression levels of HSP70 and HSP90 mRNA in supplement groups were decreased extremely significantly(P<0.01).However, there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion, the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density, sperm motility, the antioxidant enzyme activities, and decreasing the abnormal rate in hot season of Hainan.Finally, Se and VE supplement had good effects on relieving the environment heat stress.