Research Progress Towards the Liquid Chip Technology in Detection of Animal Infectious Diseases

Liquid chip technology is a novel biomolecular detection technology which integrates laser technology,flow cytometry,digital signal processing and traditional chemical technology.It is widely used in various immunological analysis and nucleic acid detection.Single and mutiplex analysis are supported,with protein and nucleic acid targets detected in a variety of detection methods in high throughput manner.It has advantages of high throughput,easy operation,wide range of application,good repeatability,high specificity,less sample needed,high sensitivity and stablility,and low cost.Therefore,they are gradually replacing the traditional detection and quantitative pathogen methods,such as Real-time fluorescent quantitative nucleic acid amplification detection system (qPCR),enzyme-linked immunosorbent assay (ELISA) and other detection methods.Animal infectious diseases seriously endanger the development of the breeding industry,futhermore,some zoonoses such as highly pathogenic avian influenza also pose a serious threat to human health.An efficient and sensitive diagnostic system will help to screen a large number of samples during the outbreak of infectious diseases and prevent the spread of infection.The development of liquid chip technology provides a new platform for high-throughput detection and disease prevention.In this review,the principle and advantages of liquid chip technology are briefly described.The research progress of liquid chip technology in the detection of animal infectious diseases,including pigs diseases,poultry diseases,rabbit diseases,dog diseases,rodent diseases and other animal infectious diseases are summarized.We believe that in the future,this technology will become an important analytical and testing tool in clinical diagnosis,basic research,new drug development,judicial identification,food health supervision,biological weapons prevention and other fields.The development of this technology will greatly promote the research and development of life science.     

室内甲醛捕捉剂

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Studies on root-produced cytokinins of inter-subspecific hybrid rice

Ｒｏｏｔｅｘｕｄａｔｅｉｓａｍａｊｏｒｉｎｄｅｘｔｈａｔｒｅｆｌｅｃｔｓｒｏｏｔｓｙｓｔｅｍａｃ ｔｉｖｉｔｙ．Ｔｏｓｔｕｄｙｔｈｅｃｈａｎｇｅｓｏｆｒｏｏｔｓｙｓｔｅｍａｃｔｉｖｉｔｙａｆｔｅｒｉｎｉｔｉａｌｈｅａｄｉｎｇｓｔａｇｅ，ｗｅｅｓｔａｂｌｉｓｈｅｄａＨＰＬＣｍｅｔｈｏｄｆｏｒａｎａｌｙｚｉｎｇｒｏｏｔ＿ｐｒｏｄｕｃｅｄｃｙｔｏｋｉｎｉｎｓｏｆｒｉｃｅｅｘｕｄａｔｅｓ，ａｎｄｕｓｅｄｔｈｉｓｍｅｔｈｏｄｔｏｍｅａｓｕｒｅｔｈｅｃｏｍｐｏｎｅｎｔｓｏｆｔｈｅｃｙｔｏｋｉｎｉｎｓ．Ｉｎｔ…     

新囊素口服液的检测方法

新囊素口服液的检测方法祁彦战石徐志华王丽１王权２吉林省兽药饲料监察所长春１３００６２；１长春市朝阳区农业局；２吉林省植保站新囊素口服液〔１〕是一种清热解毒燥湿利水的复方中药合剂，用于防治鸡新城疫、鸡传染性法氏囊炎及肠道疾病。在临床应用中取得了效果。为...     

固相萃取——高效液相色谱法分离红花中的黄酮类物质

目的 研究红花中的黄酮类物质。方法 乙醇浸泡提取，固相萃取分离，液相色谱法进行纯度检测，结果 采用的红花中有两种黄酮类物质。结论 洗脱液V（H2O）：V（C4H5OH）=3：7，能很好的分离红花中的两种黄酮类物质。     

用高效液相色谱法同时测定复方水杨酸钠注射液各成分含量

利用高效液相色谱法同时测定复方水杨酸钠注射液三种成分的含量。方法简单、快速、灵敏、准确。线性较好，ｒ值均达到０．９９９以上，回收率在９８．０～１００．０％之间，取得满意的结果     

高压液相色谱法在花色素苷分析中的应用

花色素苷是存在于许多植物红，蓝，紫色，系的母体色素，这些色素在食品品质，品性及植物生物学的化学分类标记中起着极为重要的作用，早期的研究者采用纸色谱的方法对蓊色素苷进行了研究。     

嘧草硫醚10％水剂的高效液相色谱分析

采用反相高效液相色谱外标法，以乙腈-醋酸溶液（pH3）作为流动相，用C。s柱和紫外检测器（254nm），对嘧草硫醚10％Jg剂定量分析。结果表明，方法的标准差为0．07；变异系数为0．69％；平均回收率为99．81％。线性相关系数为0．9993。     

葡萄园内套种“大蒜—夏白菜—普通白菜”

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