鱼干和鱼粉饲喂生长猪的对比效果

本试验用54头“杜长大”生长猪研究了生鱼干粉、熟鱼干粉和低价鱼粉取代日粮中常规鱼粉的饲喂效果。结果表明，生鱼干组日增重比常规鱼粉组低9.78％（P〈0.01），料肉比高5.28％；低价鱼粉组比常规鱼粉组日增重低4.6％（P〈0.05），料肉比高6.34％；熟鱼干组比常规鱼粉组日增重低2.49％（P〉0.05），料肉比高1.06％。各处理组经济效益高低顺序为：熟鱼干组〉常规鱼粉组〉低价鱼粉组〉生鱼干细。     

Establishment of Duplex Loop-mediated Isothermal Amplification Method for Simultaneous Detection of Shellfish Infected with Perkinsus and Bonamia

In order to establish a duplex loop-mediated isothermal amplification (LAMP) method for the detection of shellfish parasitic infection with Perkinsus and Bonamia, primers were designed according to the conserved region of ITS and 18S rRNA gene sequences of Perkinsus and Bonamia deposited in GenBank using the online Primer Explorer 4.0.Firstly, the respective single LAMP detection assay of two parasites were developed, and then the reaction conditions were optimized by combining the two reaction systems into duplex LAMP method.The specificity and sensitivity of duplex LAMP method were examined and the confidence was evaluated by digesting the amplicons with respective endonuclease.The results showed that the established duplex LAMP method was specific enough to distinguish the two parasites from other parasites, and the detection limit of Perkinsus was 10 copies/μL plasmid DNA, the detection limit of Bonamia was 100 copies/μL plasmid DNA.The endonuclease digestion of duplex LAMP products certified the confidence of the established duplex LAMP method.The detection result of 20 Ruditapes philippinarum samples collected randomly from the East China sea and Yellow sea and 10 Ostrea edulis samples imported from USA showed that the duplex LAMP method could be used as a rapid detection method for infection with Perkinsus and Bonamia.     

干旱胁迫对琴叶榕生长和生理特性的影响

以1a生组培琴叶榕盆栽苗为试材,控水模拟不同土壤水分状况,探讨不同程度干旱胁迫对琴叶榕叶片生理特性的影响。结果表明:随着干旱胁迫程度的提高,琴叶榕株高、净光合速率、蒸腾速率和叶绿素含量均呈现下降趋势;脯氨酸含量、丙二醛(MDA)含量都随着干旱胁迫程度的加强而显著升高;POD活性随干旱胁迫的加强而升高;SOD活性在轻度和中度干旱下上升,在严重干旱胁迫时反而下降。