断奶猪圆环病毒与猪瘟病毒混合感染的诊治

辽宁省普兰店市沙包镇,王某养猪户,饲养断奶存栏猪380头,其中于2011年6月下旬,在外地种猪繁殖场购进断奶猪,购进6天后便开始发病,已死亡7头,此时,场主聘请动物卫生监督所的兽医师到现场观察,剖检病变,实验室诊断,确诊为猪圆环病毒PCV2于猪瘟病毒混合感染。现将诊治情况介绍如下:     

几种易混淆猪病的鉴、别诊断及防制

1流行特点 猪瘟是由猪瘟病毒引起的高度接触性传染病。其特征是高热稽留和细小血管广泛性点状出血病猪病猪出现败血症或者繁殖障碍。发病率和死亡率高。不分品种、性别和年龄均易感，各季均可发生。     

一例猪蓝耳病与猪瘟混合感染的诊治

本文以实际病例为例,分析了猪蓝耳病与猪瘟混合感染的临床表现、实验室诊断和防治措施,并对发病育肥猪场的患有猪蓝耳和猪瘟混感的猪进行回顾性分析,研究该病症的临床表现和实验室诊断,并得出结论——猪瘟的漏防和免疫失败是猪发生猪蓝耳病和猪瘟混感病的重要原因之一,对猪做好疫苗工作,能够有效的降低该病的发生率,发病后需要对猪只采取科学的防控措施,具有较好的诊治效果.     

规模化猪场猪瘟发病新特征及预防

猪瘟又称猪霍乱、烂肠瘟，是由猪瘟病毒引起的一种高度接触性传染病，被定为国际检疫对象，是危害我国养猪行业的最主要的传染病之一。随着我国养殖规模化、集约化的变化，猪瘟也表现出非典型化、新生仔猪的先天性感染、混合感染严重、免疫失败等新的发病形式。为加强对猪瘟的防控，笔者着重论述了规模化猪场猪瘟的发病新特征，并提出了有效的预防措施。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

猪瘟免疫失败的原因和防制对策

猪瘟（HogCholera．HC）是猪的一种高度接触传染性病毒病。根据临床过程分为急性、慢性、迟发型三种。急性猪瘟是由强毒力猪瘟病毒引起，发病率和死亡率都很高。慢性猪瘟和亚临床型猪瘟是低毒力猪瘟病毒株感染引起，统称为非典型猪瘟，临床症状和病理变化与急性猪瘟相比，表现为多样性，而且特征很不明显，病程一般较长，易与其他疾病混淆，依靠常规方法很难确诊，     

非洲猪瘟的诊断方法及防控措施

近年来,非洲猪瘟疫情在我国东北地区出现,受到了广大养殖户的广泛关注,怎样才能及时的诊断出非洲猪瘟、如何才能做好防控工作,成为必须要研究的重要课题之一。阐述了非洲猪瘟的诊断及疾病历史,而后提出了有效的防控措施。     

某规模化猪场抗体检测分析及防控对策

探讨了规模化猪场如何开展抗体检测,并对抗体水平检测结果进行分析提出相应的防控措施。     

非洲猪瘟病毒实时荧光定量PCR检测方法的建立

为了研究检测非洲猪瘟病毒的方法,试验采用GenBank公布的非洲猪瘟病毒(ASFV)P72基因序列设计了1对特异性引物和探针,并使用含有选定检测序列的重组质粒标准品绘制标准曲线,建立了非洲猪瘟病毒实时荧光定量PCR检测方法.结果表明:该方法的敏感性可达100个拷贝值,具有良好的敏感性和重复性.     

一起猪瘟疫情的处理

2019年6月,广西某校的学生实习养猪基地发生1头母猪突然死亡,通过开展现场临床检查和病理剖解,以及采集病料送实验室检测,综合确诊为猪瘟。确诊疫病后,立即对发病猪采取消毒、深埋、隔离等无害化处理,扑灭疫情。