《农机化研究》投稿须知

<正>为防止学术不端行为和进一步提高期刊文章质量,自2011年起,本刊特对所投稿件做如下要求,望作者周知。1、文章背景要求为基金项目,并给出资助背景名称和项目号;若非基金项目,需经本刊编委初审并推荐。2、文章最短篇幅为4版(双栏,每行22字,46行,宋体5号,Word2003),要求有引     

农业远程温湿度监测装置的设计—基于NRF24LE1和GPRS

农产品的质量与其生长环境和保存环境密切相关,而农业对象又具有分散偏僻的特点,因此研究便捷、低功耗、精确的远程温湿度监测装置具有现实意义。此装置由传感节点和中继节点组成,传感节点选用NRF24 LE1低功耗器件,负责感知环境的温湿度,通过2.4 GHz无线短距传输上传至中继节点;终端PC利用GPRS远距离与中继节点通信,实时周期性收集中继节点汇聚的传感数据。传感节点与中继节点间采用防碰撞延时发送机制,实现传感节点的低功耗和数据有效传输。中继节点与终端PC通过GPRS网络建立UDP连接,使系统可以管理多个中继节点,可扩展性好。     

《农机化研究》投稿须知

<正>为防止学术不端行为和进一步提高期刊文章质量,自2011年起,本刊特对所投稿件做如下要求,望作者周知。1、文章背景要求为基金项目,并给出资助背景名称和项目号;若非基金项目,需经本刊编委初审并推荐。2、文章最短篇幅为4版(双栏,每行22字,46行,宋体5号,Word2003),要求有引     

Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.     

Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

Study on Length Polymorphism of KAP1 Family Genes in Yak (Bos gurnniens)

This study was aimed to understand the characteristics of length polymorphism with repeat sequence of keratin associated protein 1 (KAP1) family genes in yak. KAP1 family genes of yak and cattle were sequenced, and compared with sheep KAP1 family gene sequences. The results showed that cattle KAP1 family genes were located in chromosome 19, according to location of sheep KAP1 family genes in the chromosome and similarity with cattle KAP1 family genes, renaming the cattle KAP1 family (according to the gene location of chromosome) B2D, B2A, KAP1-1 and B2C genes into KAP1-4, KAP1-1, KAP1-2 and KAP1-3 gene, respectively. KAP1 family genes in the 3'and 5' flank were highly conserved, the difference between family genes mainly in the the repeat sequence region, which yak KAP1 to KAP4 genes were found 30 bp length polymorphism. There were B(CCQTS)A₁(CCQPT) repeat sequence and a new repeat sequence C(SIQTS). The results indicated that the repeat sequence was the key of the polymorphism of KAP1 family genes, which might be relate to combination with keratin protein.     

广西·桂林大果枇杷增产价扬

正本刊讯4月中旬,桂林市地产大果枇杷早熟品种(如早钟六号、解放钟)如期成熟开园上市,其市场批发价12~17元/kg,零售价16~20元/kg,至4月下旬,销售价格略有上扬。据调查获悉,2015—2016年产季桂林市大果枇杷产销主要特点:一是由于上产季桂林市大果枇杷园     

维生素B_1对饲料发酵效果影响的研究

为了弄清维生素B_1促进饲料发酵的机理及对饲料营养成分的影响,对照组饲喂基础日粮,试验组在基础日粮添加维生素B_1,均为固态发酵。结果表明:添加维生素B_1能够加快发酵进程,饲料p H和干物质含量显著降低,还原糖含量显著提高(P0.05)。饲料发酵72 h,添加维生素B_1组饲料pH降低了1.31%(P0.05),干物质降低了0.70%(P0.01),饲料还原糖提高1.34%(P0.05);维生素B_1增加了有机酸含量,改善饲料适口性,提高营养价值。     

SREBP-1c基因及其对猪肉质性状影响的研究进展

该文系统描述了固醇调节元件结合蛋白-1c(SREBP-1c)的生物学特性以及其在不同动物中的研究现状、在猪肉质方面的研究进展等,旨在进一步挖掘SREBP-1c基因作为肉质候选基因的前景性,基因与肉质性状的相关性,为肉质育种提供新的思路和方法。     

强健性秋用家蚕品种鄂蚕3号

鄂蚕3号是湖北省农业科学院经济作物研究所育成的适合长江中下游蚕区饲养的强健性斑纹双限性秋用家蚕品种。介绍了家蚕品种鄂蚕3号原种和一代杂交种的特性、饲养技术要点及注意事项。实验室鉴定成绩和农村鉴定成绩表明:该品种强健好养,壮蚕食桑旺盛,体色青白,蚕体粗壮,熟蚕上蔟齐一,茧形大,茧形椭圆形,茧色白,缩皱中等,茧层厚,抗高温多湿的能力较强。