板蓝根、黄芪对猪繁殖与呼吸综合征病毒的体外抑制作用

为了解中草药黄芪、板蓝根对病毒增殖的抑制作用,对黄芪、板蓝根及二者联合使用在Marc-145的单层细胞上对猪繁殖与呼吸综合征病毒(PRRSV)增殖的抑制作用进行了研究。结果表明,板蓝根能够显著抑制PRRSV体外增殖,对PRRSV的最小直接杀灭浓度为0.195 mg/mL,最小阻断浓度为0.097 mg/mL;黄芪对PRRSV增殖的抑制作用较弱。板蓝根、黄芪联合使用时对PRRSV的抑制作用显著增强,板蓝根对PRRSV的最小直接杀灭浓度降为0.097 mg/mL,最小阻断浓度降为0.049 mg/mL。     

中药添加剂对肉杂鸡血清生化指标的影响

本试验旨在从血清生化指标变化的角度研究中药添加剂促肉杂鸡生产的作用机理。 1 材料与方法 1．1 中药与实验动物 中药添加剂是由黄芪、麦饭石、板蓝根、淫羊藿、麦饭石粉等组成，并分别粉碎过40目筛，取各药粉末按设计比例充分混合即成HMS散剂。肉杂鸡的父系为罗斯308肉鸡，母系为海蓝蛋鸡。     

板蓝根的栽培技术

板蓝根别名菘蓝,是两年生草本植物,一种常用中草药,根和叶均可入药。性大寒,味苦,能清热解毒,凉血,是治疗感冒的常用药。还可以治疗流行性腮腺炎、流行性癔性脑炎、流行性脑脊髓炎、传染性肝炎、白喉等病。板蓝根高35～80cm,上部多分枝,常带白粉,叶互生,基生叶较大有柄,叶片椭圆形,茎生叶长圆形,向上渐小,基部箭形,半抱茎,全缘或微有细齿。     

犬猫对某些中药注射液的过敏反应

<正>家畜对药物过敏在兽医临床上屡见不鲜,多见于合成药物,但天然药物引起过敏则很少见。由于家畜对中草药制剂过敏的特异性试验尚属空白,并且临床上对病畜药物过敏完全无预见能     

板蓝根栽培技术

板蓝根为十字花科植物菘蓝或爵床科植物马蓝的干燥根,具有清热、解毒、凉血功能,为常用中药材。其栽培技术如下。一、选地整地。选择土层深厚、疏松、肥沃、排水良好的壤土或砂质壤土,深耕30厘米,每667平方米施厩肥和草皮灰混合3000千克作基肥,犁耙1次,使土壤与肥料拌匀。松碎后起高20～25厘米,宽1～1.3米的畦。二、繁殖方法。采用种子繁殖。5～6月板蓝根果皮由黄白色变为蓝紫色     

板蓝根合病毒灵治疗蛋鸡传染性支气管炎

鸡传染性支气管炎是由病毒引起的一种急性、高度接触性呼吸道传染病。其特征是病鸡咳嗽、流鼻液、打喷嚏和气管发出啰音;感染鸡生长受阻、耗料增加、产蛋和蛋质下降、死淘率增加,     

杂交玉米间套板蓝根种植技术

通过杂交玉米间套板蓝根示范种植,可让农民增产增收,并有效的解决了旱地分带轮作中副带作物的套种问题。     

养出276元1公斤的鸡

高健,浙江省安吉县人,1999年下岗后觉得中草药养鸡是个卖点.于是就从上海交通大学买回了中草药养鸡的技术专利,养起了药材鸡.     

Study on the Anti-Escherichia coli Activity and Mechanism of Water Extract of Radix isatidis Powder

In order to explore the antibacterial activity and mechanism of the Radix isatidis powder water extract,the effects of the Radix isatidis powder water extract on the morphology and structure of Escherichia coli (E.coli) were tested by scanning electron microscopy and transmission electron microscopy.The effects of the Radix isatidis powder water extract on the conductivity and total leakage rate of E.coli and the content of alkaline phosphatase in the culture medium of the content of protein,DNA and RNA were stained by DAPI to detect the effect of the Radix isatidis powder water extract on the nucleic acid of E.coli,and the effect of the Radix isatidis powder water extract on the metabolism of E.coli in vivo,in vitro,ALT,AST,pyruvic acid and ATP.The results showed that after the Radix isatidis powder water extract acted on E.coli for 10 h,it was observed by SEM that the bacteria appeared to overflow and shrink,the length of the bacteria became shorter obviously,many residues were formed due to breakage,some of them were sunken in the middle and deformed.Under TEM,it was observed that the boundary of the cell wall of E.coli was unclear,the wall membrane was zigzag,deformed and some of the bacteria were broken.The protein content outside the cell was significantly different from that in the blank control group from 8 h (P<0.01), and that in the cell from 4 h (P<0.01). DNA content had no significant difference with blank control group before 12 h (P>0.05), but had significant difference with blank control group from 16 h (P<0.01); RNA content began to decrease at 8 h, and was significantly different from blank control group at 12 h (P<0.05), and was extremely significant from 16 h (P<0.01). There was no significant difference between ALT and AST (P>0.05). The pyruvate content in culture medium and bacteria was higher than that in blank control group, and the difference was very significant from 4 h (P<0.01). The ATP content in the culture medium was significantly different from that in the blank control group (P<0.01), and the ATP content in the cell was significantly different from that in the blank control group from 4 h (P<0.01).In conclusion,the Radix isatidis powder water extract could inhibit the synthesis and metabolism of bacterial genetic material and the content of pyruvate and ATP by destroying the integrity of cell wall and cell membrane.