转MsLEA4-4基因拟南芥株系的耐盐性分析

为了深入研究紫花苜蓿（Medicago sativa）MsLEA4-4基因的抗逆性，本试验对前期获得的转MsLEA4-4基因的拟南芥（Arabidopsis thaliana）株系进行不同浓度盐胁迫处理，以验证MsLEA4-4基因在拟南芥植株体内的耐盐性。试验结果表明：盐胁迫后转基因株系的发芽率达到55%，比对照植株（Control plant，CK）高81%；转基因株系的鲜重、叶绿素含量均高于CK（P<0.05）；盐胁迫后转基因株系的存活率比CK提高75.0%~81.3%；盐胁迫明显抑制根系生长，但转基因植株的侧根数量比CK多4~5根（P<0.05）；盐胁迫后转基因株系体内的可溶性糖含量（P<0.05）和超氧化物歧化酶（P<0.01），过氧化氢酶（P<0.01），过氧化物酶（P<0.05）活性均高于CK，脯氨酸（P<0.01）和丙二醛（P<0.05）含量均低于CK。综上可得，MsLEA4-4基因参与了拟南芥体内的耐盐性调控，提高了转基因拟南芥对盐胁迫的耐受性。本研究为进一步探索MsLEA4-4在转基因紫花苜蓿盐胁迫中的功能及培育耐盐性紫花苜蓿品种提供依据。     

Preparation of Atherosclerosis Model Using Guangxi Bama Mini-pig

The atherosclerosis (AS) model was prepared in Guangxi Bama mini-pig,and the atherosclerosis index (AI) was preliminarily identified in occurrence of AS to provide the basis for the preparation of related models.20 Guangxi Bama mini-pigs were randomly divided into control group and experimental group which were fed with high fat and cholesterol diet to prepare the AS model.Blood biochemical indexes were detected in the process of model preparation and the correlation between AI and the results of vascular slices was analyzed to initially draw up AI of AS.The results of vascular slices showed that the incidence rates of AS of Guangxi Bama mini-pigs in experimental group and control group were 20% and 0,respectively.The association analysis between the results of vascular slices and AI in the pathogenic Guangxi Bama mini-pigs preliminarily suggested that AI was above 3.8 and lasted for more than 3 months during the attack.     

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.     

枣保1号对壶瓶枣3种主要病害的防效

为了研究枣保1号在防控壶瓶枣黑顶病的同时对缩果病和裂果的综合防效,于2014年7—9月,在山西省晋中市太谷县小白乡红枣基地选取试验枣园,喷施了4次浓度为0.75%的防控剂——枣保1号,在枣果近成熟期调查枣黑顶病、缩果病、裂果的发病率和病情指数。结果表明,喷施枣保1号在极显著降低壶瓶枣黑顶病的同时,也可以极显著地降低缩果病和裂果的发病率和病情指数,对3种病害的平均防效分别达74.49%±1.16%,76.40%±11.48%和67.07%±7.95%。说明枣保1号在防控枣黑顶病的同时对缩果病、裂果也有很好的防效,为枣保1号的进一步推广提供了理论依据。     

薄壳山核桃新品种——亚优YLC28号

“亚优YLC28号”是从薄壳山核桃实生大树中选育出的新品种。2019年12月通过浙江省林木品种审定委员会审定。果实较大,坚果平均重13.9 g,纵径57.27 mm,横径33.59 mm,果形指数1.70,果仁平均重7.08 g,出仁率50.94%;核仁含脂肪60.5%,蛋白质56.40 mg/g,果仁色美味香、松脆,无涩味,营养丰富。     

不同抗线番茄红果品种筛选试验

针对宁夏设施土壤连作障碍引起的作物根结线虫问题,以番茄品种"赛琳娜"为对照,引进"9029"、"福莱德"、"TY-2"、"TY-3"、"的奥特"、"NUN3734"、"大红7845"、"SV3660TH"、"SV7845TH"、"卓雅309"等10个抗线番茄品种,系统研究了不同品种植株长势、果实品质、始花结位和根系病级、土壤根结线虫数量的变化,以期筛选出丰产质优的抗线番茄红果品种。结果表明:10个引进品种的田间抗病性均较好;"9029"和"NUN3734"始花节位较低,分别为24.0cm和29.5cm,植株长势较好,土壤线虫数较低,分别为87条/100g干土和74条/100g干土,抗病性强、丰产性强,因此,"9029"和"NUN3734"果实中等匀实,抗根结线虫强,植株长势好,可作为高产优质抗线番茄粉果品种进行推广。