Sequencing, Polymorphism and Expression Profile Analysis of Porcine Hexokinase Ⅱ (HK2) Gene

Hexokinase Ⅱ has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes the conversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, the partial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics.Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms (SNPs), one of which in intron 10 with differing bases (G981A) is within the restriction site for enzyme Msp Ⅰ. Distribution of Msp Ⅰ -RFLP genotype and allele frequencies among different pig breeds were studied. By association analysis between Msp Ⅰ PCR-RFLP polymorphism (AA, AB, BB genotypes of HK2 gene intron 10) and some meat quality and carcass traits in F2 group,which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between AB and BB genotypes (P ＜ 0 05) in F2 group. In addition, the pattern of expression of HK2 in a variety of tissues in pig was also determined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle.     

百可宁对鸡传染性喉气管炎的疗效观察

以中兽医理论为指导，经临床反复试治和实验，优化组方筛选出了中草药复方制剂——百可宁。为了进一步探讨该药对鸡传染性喉气管炎的疗效，我们进行了人工感染和自然感染治疗试验。     

杂交水稻Ⅱ优084母本旱育抛秧高产制种技术

三系杂交稻新组合Ⅱ优084(Ⅱ-32A/镇恢084)具有优质、高产、高抗白叶枯病的特点.介绍了其旱育抛秧高产制种技术.     

复合微生态制剂在水产养殖中的应用

随着水产养殖的迅猛发展和集约化经营程度的不断提高，渔业水域污染日趋严重，许多养殖池中有害藻类及病菌大量繁殖，水质条件不断恶化，已成为水生动物病害暴发的主要原因之一。另外，抗生素的滥用不仅损害水产动物的健康，而且产生了严重的耐药性。为了观察复合微生态制剂在水产动物上的使用效果，我们分别在甲鱼和对虾饲料中添加该制剂进行试验。     

蔬菜病虫害非农药防治技术

播种前，晴天将蔬菜种子晒2天～3天．利用阳光杀灭附在种子表面的病菌，减少发病。瓜类、茄果类蔬菜的种子用55℃温水浸种10分种～15分钟，豆科和十字花科蔬菜的种子用40％～45℃温水浸种10分钟～15分钟，均可消毒杀菌，预防苗期病害。     

锌制剂防治牛羊腐蹄病研究进展

腐蹄病是牛、羊的常见蹄病。兽医临床上,通常采用修蹄、硫酸铜或甲醛溶液浴蹄和抗菌治疗等措施防治腐蹄病。二十世纪七十年代以来,国内外兽医工作者采用锌制剂防治牛、羊腐蹄病,取得了良好的效果。涉及到的锌制剂有硫酸锌、氧化锌等,采用的方法有将锌制剂添加在饲料中饲喂或含锌盐砖舔食、含锌溶液浴蹄或浸蹄、肌肉注射等。现就锌制剂对牛、羊腐蹄病的防治作用及与此相关的一些问题作一简要综述。1对牛、羊腐蹄病的防治作用1.1口服补锌的防治作用1973年,Demertzis和Mills报道经口给予硫酸锌减少了青年公牛蹄皮炎的发病率,每日每公斤体重饲…     

“油菜等重要经济作物促生／生防细菌、制剂和应用”通过成果鉴定

2005年元月20日，由中国农业科学院油料作物研究所主持承担的国家科技攻关重点课题和武汉市科技攻关项目“油菜等重要经济作物促生／生防细菌、制剂和应用”项目通过了武汉市科技局组织的成果鉴定。