海南地方猪IGFBP6基因多态性分析

采用PCR-SSCP技术,对五指山猪、海南地方猪2个品系(临高猪、屯昌猪)等猪种的IGFBP6基因的多态性进行研究。结果表明,引物P1和P2的PCR片断SSCP分析表现有多态性,且出现3种基因型。随后的测序结果表明,它们都在相应的区域存在着变异;引物P1在海南地方猪种大多表现BB基因型和以B等位基因为主,引物P2大多表现CC基因型和以C等位基因为主。SPSS软件分析五指山猪IGFBP6基因多态性与其3个生长阶段的关联程度,结果发现,引物P1 AA基因型和引物P2 DD基因型的猪个体在2月龄、8月龄、12月龄的生长体重高于其它基因型,但个体差异均不显著(P0.05)。     

Pathogenicity of a Strain of H5N6 Subtype Avian Influenza Virus in Ducks and Mice

To investigate the pathogenicity of A/Hero/Guangdong/C1/2013(H5N6), an AIV strain isolated from Heron on ducks and mice, pathogenicity of this new virus strain and changing of histopathology as well as a preliminary study on its biological characteristics were studied by virus challenges test via intranasal and eye-drop and in chicken via jugular vein injection.Results showed that the EID₅₀ of this strain of virus was 10^-8.16/0.1 mL in embryonated chicken eggs and the intravenous inoculation of pathogenic index (IVPI) was 2.76.In ducks and mice, the 50% lethal doses (LD₅₀) of it were determined to be 10^-4.0/0.2 mL and 10^-4.67/0.05 mL, respectively.Symptoms of infection included loss of appetite, depression, swollen head and tears after being infected with 10⁶ EID₅₀ per duck by intranasal and eye-drop administration.Most of ducks died 4 to 7 days post-infection, liver, lung and kidney still eliminated toxicants at day 7 post-infection.Anatomy showed symptoms of pericardial effusion, pulmonary congestion and kidney enlargement, while pathological section showed pathological change like karyopycnosis and inflammatory cell infiltration in heart, liver, kidney and spleen.Mice developed symptoms of infection like loss of appetite, depression, shaggy coat and ruffled coat after being infected with 5×10⁵ EID₅₀ per mouse by intranasal and eye-drop administration.Most of the infected mice died 5 to 7 days post-infection and only liver still eliminated toxicants at day 7 post-infection.Although organ anatomy showed no obvious pathological changes, pathological section showed pathological changes like karyopycnosis and inflammatory cell infiltration in heart, kidney and lung.Our research demonstrated that this H5N6 subtype AIV had a strong pathogenicity and could be defined as a highly pathogenic AIV strain as its IVPI was greater than 1.2.Our work laid a theory foundation for study, prevention and control of H5N6 subtype AIV.     

秋大豆新品种“赣豆6号”的选育与应用

“赣豆6号”是利用高产、早熟、适应性广的“82N10”作母本,质优、抗逆性强的“8415-8”为父本进行杂交,经多年选育而成的一个优质、高产、抗逆性强、适应性广的秋大豆新品种。通过参加江西省秋大豆新品种区域试验,“赣豆6号”平均产量为2477.18kg/hm2,比对照“赣豆3号”增产5.47%,全生育期95d左右。     

谈春小麦高产栽培

<正>目前栽培的小麦品种,新春6号,永良15号。春性品种的种子在土壤里萌动以后,必须经过一定时间的低温条件,才能起身拔节发育形成结实器官,这段时间叫做小麦的春化阶段。春性品种通过春化阶段的温度较高,一般是在7℃～15℃,5～8天就能通过春化。一、栽培春小麦须注意的几点事项(一)解决春化问题,为了使小麦尽快通过春化及时成熟,可采用下列方法:1.早播种。春节前后,遇到气温较高的时段,当地表化     

Dramatic Changes of Matrix Metalloproteinases-7 and Lysozyme in the Ulcerative Colitis of Mice Induced by Dextran Sulfate Sodium

Ulcerative colitis （UC） is a lifelong illness with profound emotional and social impacts, and could cause serious damage to large intestine, especially in colon. However, the pathogenesis of UC remained unclear. The present study attempts to find out the role of matrix metalloproteinases-7 （MMP-7） and lysozyme in the pathogenesis of UC through a mice model induced by dextran sulfate sodium （DSS）. The UC model was evaluated both by disease activity index （DAI） and the intestinal histopathology. The results show that there is a high correlation between the DAI score and the pathological changes of colon. Interleukin-6 （IL-6） serum levels and large intestinal fluids levels in UC mice are always higher than that of the control groups, which might be associated with the degree of the inflammation damage in the colon. The change tendency of the MMP-7 mRNA and protein expressions are both up-regulated firstly and then down-regulated from 1 to 5 d in the colon, but only the MMP-7 protein is up-regulated at 7 d again. The up-regulated MMP-7 levels in the early stage of UC may play a protective role through the activated defensins, while the down-regulated levels in the mid-later stage of UC may be connected with the severe lesions in the colon. However, the up-regulated MMP-7 levels in the later stage of UC in the colon may also contribute to the tissue repair or be served as a marker to CRC （colorectal cancer）. The distribution of lysozyme protein indicates that there may be Paneth-like cells in the colon. Both the changes of MMP-7 and lysozyme in the small intestine may play a protective role for the safe environment of the whole gut, especially to the colon of UC.     

5个芒果品种在广西龙州的引种试验

2008年在广西龙州县引种栽培热农1号、热农2号、西贡芒、红芒6号、金煌芒共5个芒果品种。经过田间观察比较,热农2号抗逆性强,丰产性好,果实中等,肉质细腻,香甜多汁,纤维中等,品质优良;金煌芒抗逆性强,丰产性好,果实特大,外观好,晚熟,耐贮运,味甜多汁,纤维少,品质好,这2个品种可在龙州县推广种植。     

春季栽培甜玉米品种鄂甜玉6号的种植密度筛选试验

为了研究春季栽培时,不同定植密度对甜玉米品种鄂甜玉6号主要农艺性状、产量及品质的影响,对2800、3200、3500、4000株／667m^2 4个种植密度进行了比较试验,结果显示,春季栽培时3200～3500株／667m^2为鄂甜玉6号最适宜的种植密度．其产量较高。     

PB6活性菌株及其特性的研究

枯草芽孢杆菌PB6菌株是由建明工业(Kemin Industries)生产,在美国FDA注册的从健康鸡肠道中分离出来的益生菌。PB6活性菌株能通过分泌细菌素,直接杀灭魏氏梭菌(产气荚膜梭菌),并抑制大肠杆菌、沙门氏菌等需氧菌的生长,促进乳酸杆菌、双歧杆菌等有益厌氧菌的增殖,从而防止由梭菌引起的坏死性肠炎的爆发。同时,PB6活性菌株还能抵御高温的破坏,与生产应用上常用的酸、抗生素兼容。     

Jeziorny法分析尼龙MXD6非等温结晶行为

本文用差热扫描量热仪对MXD6进行了非等温结晶动力学的研究,并用Jeziorny法对测试结果进行了分析,结果表明Avrami指数的范围是2.3-3.3即在3左右,这就说明MXD6结晶特点为多维异相成核。     

应用“1书1网1＋6”保障养殖安全生产

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