桦木酸抑制地塞米松诱导脾淋巴细胞凋亡的作用

研究桦木酸对脾淋巴细胞凋亡的影响。以地塞米松建立小鼠体外脾淋巴细胞凋亡模型,利用倒置显微镜、荧光显微镜和电子透射显微镜观察细胞形态和亚细胞结构,琼脂糖凝胶电泳观察DNA的裂解,流式细胞术测定细胞凋亡率,检测华木酸对Dex介导的小鼠脾淋巴细胞凋亡的影响。结果表明,2.5μmol Dex可使脾淋巴细胞凋亡明显增加,染色质发生边聚、浓缩、凋亡小体等典型的凋亡特征,桦木酸可明显降低地塞米松引起的脾淋巴细胞凋亡。提示桦木酸对地塞米松诱导的淋巴细胞凋亡具有预防性保护作用。     

牛博士热线

大象好！我是牛博士,今天给大家带来的主要是奶缸牛奶体细胞检测在牧场管理中的应用方面的相关知识,请大家仔细阅读!检测奶缸牛奶的体细胞可以了解牛群乳房炎感染状况．奶缸牛奶体细胞数超过20万／毫升,说明牛群中有明显的乳房炎流行。尽管有的牛场体细胞非常高,超过50万／毫升．有的甚至超过100万／毫升,而低于20万／毫升是个实际目标。了解奶缸体细胞数有助于分析感染牛的感染性质,这类感染一般呈隐性状态,而且存在时间长,     

家兔腹泻原因及防治

1 消化不良性腹泻 此类腹泻多发生于断奶前后的仔兔. 1.1 发病原因 断奶前后的仔兔消化器官发育尚不完全,开食过早或采食难以消化的粗硬饲料时易发生腹胀腹泻.     

Research on the Antioxident Function of Sulforaphane on the Leydig Cells of Cadmium Exposured Mice

The study was aimed to explore the antioxident function of sulforaphane (SFN) on the leydig cells of cadmium (Cd) exposured mice.Cd and SFN were added to the cell culture medium of TM3 cell, half inhibitory concentration (IC₅₀) of Cd and SFN safe dose range were determined.The tested model was set up,and the relative survival rate of TM3 cells, lactate dehydrogenase (LDH) activity and cell antioxidant levels were determined to study the antioxident function of SFN to cadmium exposured mice.The results showed that:①With the increase of Cd concentration,the relative survival rate of TM3 cells was decreased,and the IC₅₀ of Cd was 51.4 μmol/L;Within a certain concentration range,SFN could increased the cells survive rate,but there was toxicity when the SFN concentration was more than the scope,and the greater the concentration,the greater the toxicity. At experimental condition,SFN safety concentration were 2.5,5 and 10 μmol/L.②Compared with the control group,the GSH content,the T-SOD and GSH-Px activities in Cd group were significantly or extremely significantly decreased (P < 0.05;P < 0.01),and the LDH activity,the MDA content were increased.Additionally,the LDH activity and MDA content of SFN groups were decreased,while others three indexes were increased. Compared with Cd group,the GSH content,the T-SOD and GSH-Px activities of Cd+SFN groups were significantly or extremely significantly increased (P < 0.05;P < 0.01),however,the LDH activity,MDA content were decreased. The result indicated that SFN had the antagonism effect on toxicity of Cd in TM3 cell.     

兔痘的临床症状与剖检特征

兔痘是由兔痘病毒引起的家兔的一种热性、急性、高度接触性的传染病。该病以皮肤出现红斑、丘疹,眼炎,以及内脏器官发生结节性坏死为典型临床特征。兔痘病毒属正痘病毒科痘病毒属。该病毒可在多种动物培养细胞如H细胞、猪肾细胞、     

鸡群缺乏维生素D3的危害及饲料中维生素D3的添加

1骨骼矿化缺乏维生素D3或者缺乏其代谢物,会导致钙化过程障碍,从而会造成骨骼软弱,尤其在快速生长的鸡更是这样。商品肉鸡群中的所谓＂弱腿症＂在一定程度上就是由于钙三醇的生物合成量不足以满足骨骼发育的生理需要。肉鸡群中最常见的病症就是胫骨软骨发育不良症和股骨变性。     

老芒麦种子发育过程中的生理生化变化

在河北省承德市鱼儿山牧中国农业大学科技攻关实验站,以种植２年和３年的老芒麦为材料,研究种子发育过程中的生理化变化。结果表明,在老芒麦种子发育过程中含水量均呈先慢后快的阶段性下降,而干重则逐渐增加,种子在盛花期后２６～２７ｄ达到生理成熟,含水量为３９．０～４５．６％。可溶性粮含量随种子发育成熟逐渐减少,相反,淀粉含量则逐渐增加,生长素、赤霉素、脱落酸含量高峰在种子发育过程中出现的顺序不同。     

PK15细胞在不同培养条件下的生长情况

PK15细胞在多种兽用疫苗的研究和生产中被广泛培养。在生产中培养生长状态良好的PK15细胞,是生产优质疫苗的前提。随着疫苗生产工艺的不断更新迭代,细胞培养的方式也不断增加,目前常见的培养方式有方瓶、转瓶、生物反应器等。为了给生产制定更加优化的培养策略,本文探讨了PK15细胞在方瓶、转瓶、生物反应器三种不同培养条件下的生长情况。通过分析每次细胞培养前的分种密度、培养72 h细胞密度、细胞增长倍数并观察24 h、48 h、72 h的细胞状态,确定细胞的生长情况。结果表明,就对于细胞生长繁殖而言,无论哪种培养方法,该细胞均能很好生长。培养72 h,就细胞增长倍数而言,各培养系统有所区别,表现为方瓶倍增为10～30倍,转瓶为5～10倍,生物反应器为8～12倍。     

影响北极狐精液品质的几个因素

1生殖器官畸形由于隐性基因的作用或胎儿期的某个阶段发育受阻而使公狐形成隐睾,睾丸大小不均匀,睾丸小而硬等生殖器官畸形和性腺发育不全,均不能产生正常精子,或所产精子多为畸形、弱精、死精,都不宜作种用。     

猪链球菌病高免卵黄抗体的制备及应用

猪链球菌病是由多种不同群的链球菌引起的不同临床类型传染病的总称。常见的有败血性和淋巴结脓肿链球菌病,前者由C群链球菌引起,发病率和死亡率高,危害大,断奶后哺育期仔猪的死亡率高达4%-5%,有的甚至高达15%;后者由E群链球菌引起,临床上比较常见而且流行广。