泾川县蔬菜生产供给保障能力建设的思考与对策

介绍了泾川县加快推进蔬菜生产供给保障能力建设情况,分析存在问题及原因,提出了建议与对策。     

DHI技术在安达友谊牧场的应用效果

黑龙江省安达友谊牧场自2007年参加DHI体系以来,奶牛场的管理水平和经济效益都得到了很大提高。本文通过对DHI报告数据的分析,主要从显著提高产奶量,降低乳房炎发病率,改进日粮结构等方面概述了该牛场取得的成果。     

云南农大培育出10头转基因克隆猪

本刊辑：据都市时报2011年2月6日报道，云南农业大学最近培育出10头转基因克隆猪。 云南农业大学转基因克隆猪项目总负责人曾养志介绍说，这10头转基因克隆猪都是体细胞繁殖，它们是从英国大白猪的皮肤细胞中分离出细胞，经过培养后，把Leptin基因和水母绿色荧光蛋白基因构建到同一个载体上，获得供体细胞，再进一步构建出转基因克隆胚胎。     

对大兴安岭地区湿地资源保护的思考

湿地是自然界富有较高生物多样性和生产力的生态系统，不但具有丰富的资源，还有巨大的环境调节功能和生态效益。各类湿地在提供水资源、调节气候、涵养水源、均化供水、促淤造陆、降解污染物、保护生物多样性和为人类提供生产、生活资源等方面都有重要的意义，被誉为“地球之肾”、“生命的摇篮”等，每年的2月2日为世界湿地日。     

湖北奶牛生产性能测定

奶牛生产性能测定，又称奶牛群遗传改良（DairyHerdImprovement，DHI），是目前世界上最为科学、最为有效的奶牛生产管理工具。其主要工艺为：对每头泌乳牛每月采集一天的奶样，在测定其产奶量、乳成分、体细胞数等数据的基础上，收集牛群饲养管理数据（如产犊日期、干奶日期、淘汰日期、年龄、胎次等），再将这些数据加工处理，形成DHI报告，返回牛场用以指导生产、经营和管理。     

用2,4-D处理获得可育甘薯组种间杂种

用50mg/L 2,4-D处理杂交不亲和组合甘薯(Ipomoea batatas (L.)Lam.)品种徐薯18(2n＝6x＝90)×L.lacunosa(2n＝2x＝30)以及I.trifida(2n＝4x＝60)×L triloba(2n＝2x＝30)的母本花器,获得了可育种间杂种植株,并对种间杂种进行了过氧化物酶同工酶分析和细胞学观察.特别是徐薯18×I.lacunosa的杂种后代具有明显的结薯性,这是首次报道获得具有结薯性的甘薯品种和第Ⅱ群近缘野生种种间杂种.     

大汶河水资源优化调度

随着泰安市社会经济的快速发展及城市化进程的加快,对水资源的需求量越来越高。水资源短缺和水质污染已严重制约了社会经济的发展和人民生活质量的提高。因此,合理调度大汶河水资源对于流域水资源、生态环境与社会经济协调发展有重要的现实意义。     

影响智能表台区线损因素的分析

<正>智能表改造完成对台区降损起到了极大的作用,但也存在部分智能表改造台区线损率下降不明显,甚至反而升高的情况。笔者现结合在对线损异常的智能表台区核查过程中发现的问题举例浅谈。1线路运维与营抄脱节某供电所为解决供区内一小水电家属区台区变压器超载问题,在离该台区50 m距离的地方新增了1台公用变压器为审计局台区,原线路在2台区中间进行了切割。在此方式运行1年后,供电所为解决这2个台区负荷不均匀问题,将小水电家属区台区的2栋楼房77户的供     

Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.     

植物体细胞胚发生的激素调控

随着生物科技的不断发展,植物体细胞胚胎发生的技术随之兴起,该技术的应用不仅让植物快速繁殖的目标得以实现,同时为植物胚胎发生机理、遗传转化系统的形成提供了帮助。本文通过植物五大激素对植物体细胞胚胎发生的调控及其作用机制的介绍进一步对其研究进展进行探讨。