冬小麦新品种唐麦6号的选育与应用

以京411为母本,河农215为父本杂交,通过单倍体育种方法选育出适合于冀东、京津地区种植的冬小麦新品种唐麦6号。该品种具有抗寒、抗病、优质、高产等特性,一般产量为6000～7000kg/hm2,最高产量可超过7500kg/hm2。2003年4月通过河北省农作物品种评审委员会审定。     

山羊血乳酸值及血样保存时间对其含量影响

采用对羟基联苯比色法对３５只青海土种山羊血乳酸于采血后第２天和第８天进行测定，结果：被检土种山羊血乳酸值为２．３８±１．６２ｍ ｍｏｌ／Ｌ，而且在性别、年龄组间无显著差异；血样保存２天和８天时间对血乳酸值也无显著影响。     

Identification of allelic variation in the atp6 gene and its use in developing molecular markers for distinguishing two cytoplasmic types of bunching onion (Allium fistulosum L.)

The atp6 is a significant candidate gene that may be related to cytoplasmic male sterility (CMS) in higher plants. In this study, atp6 gene fragments were cloned from the CMS line and its maintainer line in bunching onion. The primers were designed according to sequences obtained from GenBank accession no. JQ283733. An important variation between the CMS and maintainer line was observed at the 171T and 171A position, respectively. The atp6 genes obtained from the CMS line had 171T mutations designated as T-atp6 (GenBank accession no. KR973431), and the maintainer line had 171A mutations designated as A-atp6 (GenBank accession no. KR973430). Furthermore, some plants in the maintainer line possessed both 171A and 171T mutations designated as heteroplasmic genotype A/T-atp6. To confirm this point mutation site, the derived cleaved amplified polymorphic sequence (dCAPS) method was used. Consequently, we developed a competitive allele-specific PCR (KASP) marker based on the observed single nucleotide polymorphisms (SNPs) in the atp6 genes. This marker will allow breeders to identify male-sterile or fertile cytoplasmic types on a large scale and may significantly contribute to the breeding of bunching onion F₁ hybrid cultivars.     

Secondary school students’ perspectives and use of three school grounds of varying size,content and design

School grounds can have multiple values, and especially sufficient size, green elements, variation, integrated design and participatory development have been described as positive qualities. However, many studies have focused on pre-school and primary school grounds, while less is known about school grounds for adolescents. This study explored how secondary school students experience and use school grounds of varying size, content and design. The study included one class in year 8 (aged 14–15) at each of three schools in southern Sweden. A questionnaire with mainly open questions was distributed, followed by both individual interviews and walking interviews with four students from each class. The results show that school grounds were appreciated, but also that many chose to stay indoors. Large surface area and varied content, with ball courts, greenery, seating and multifunctional equipment, were valued, but a school ground design with integrated and pleasant settings, allowing socialising and activities, particularly support use by secondary school students.     

Interaction of neuropeptide Y receptors (NPY1, NPY2 and NPY5) with somatostatin on somatostatin-induced feeding behaviour in neonatal chicken

1. The present study was conducted to investigate whether brain somatostatin increases feed intake in neonatal chickens. The mediating role of neuropeptide Y receptors on feed intake induced by somatostatin was investigated.

2. In this study, seven experiments were designed, each with four treatment groups (n = 44 in each experiment). In Experiment 1, chicks received control solution and 0.5, 1 and 2 nmol of somatostatin through intracerebroventricular (ICV) injection. In experiments 2, 3 and 4, chickens were ICV injected with control solution and 1.25, 2.5 and 5 μg of B5063 (NPY₁ receptor antagonist), SF22 (NPY₂ receptor antagonist) and SML0891 (NPY₅ receptor antagonist), respectively. In experiment 5, 6 and 7 chickens received ICV injection of B5063, SF22, SML0891, with a co-injection of + somatostatin, control solution and somatostatin. The cumulative feed intake was measured until 120 min post injection.

3. Somatostatin significantly increased feed intake in FD3 chicks. Both B5063 and SML0891 dose-dependently decreased feed intake compared with the control group, while SF22 led to a dose-dependent increase in feed intake. In addition, the hyperphagic effect of somatostatin significantly decreased with co-injection of B560 plus somatostatin (p < 0.05), but SF22 and SML0891 had no effect on feed intake induced by somatostatin in chicks (p > 0.05).

4. Based on the results of this study, it is likely that somatostatin increased feed intake and NPY₁ receptor acts as a mediator in hyperphagic effect of somatostatin in neonatal chicks.     

黄樱桃营养袋全光照弥雾嫩枝扦插技术

研究了GGR-6对黄樱桃全光照弥雾嫩枝扦插的影响.结果表明:GGR-6 处理显著(P＜0.05)提高了黄樱桃插条生根率,明显缩短了生根时间.GGR-6 100 mg/kg处理生根效果最好,插条的生根率、平均生根数和平均根长分别比对照增加了37.5%、392.0%、80.0%,生根时间比对照缩短11 d.     

饲养方式和卢氏绿壳蛋鸡经济效益的相关性

讨论不同饲养方式对卢氏绿壳蛋鸡体重、屠宰性能、消化器官、肠道组织的影响。本试验选取卢氏绿壳蛋鸡200只,其中放养组和笼养组各100只,且每组公鸡、母鸡各50只,对其相关指标进行测定分析。结果表明:放养鸡的各项指标都优于笼养鸡,产出效益较高,同时放养鸡具有较强的自由觅食能力,能起到降低成本的效果。通过经济性比较,养殖企业选择科学的管理方式有助于提高经济效益,增强市场竞争力,促进企业生存和发展。     

枸杞多糖对免疫抑制小鼠血清中IL-6、IL-10和IL-12/IL23 p40分泌水平的影响

通过研究枸杞多糖(LBP)对免疫抑制小鼠血清中细胞因子IL-6、IL-12/IL-23p40和IL-10分泌的影响,探讨LBP的免疫调节机制,为LBP的开发利用提供理论依据。60只ICR雌性小鼠,随机分为5组,对各组小鼠进行连续4d每日腹腔注射环磷酰胺(40mg/kg),间隔3d后,再连续4d每日腹腔注射环磷酰胺(40mg/kg);同时,用高(40mg/1kg)、中(20mg/1kg)、低剂量(10mg/1kg)的LBP给小鼠连续灌胃2周,阳性对照和阴性对照分别用LPS(1mg/kg)和PBS,连续2周。小鼠眼眶取血,分离血清,ELISA检测血清中IL-6、IL-12/IL-23p40和IL-10的分泌水平。结果显示,与阳性对照和阴性对照组相比,LBP能提高免疫抑制小鼠血清中IL-6、IL-12/IL-23p40和IL-10的分泌水平,提示LBP能增强小鼠细胞免疫与体液免疫应答,为枸杞多糖作为免疫增强剂的开发应用奠定了一定的理论基础。