归参汤对免疫抑制小鼠血清中IL-2、TNF-α和脾细胞Th1/Th2细胞因子的影响

为了探讨归参汤(GST)对免疫抑制小鼠血清中IL-2、TNF-α和脾细胞Th1/Th2细胞因子的影响。本试验采用环磷酰胺制造免疫抑制模型,利用双抗夹心ELISA法,观察归参汤对小鼠血清中IL-2、TNF-α的影响;RT-PCR法观察归参汤对小鼠脾细胞中INF-γ、IL-4、T-bet和GATA-3的mRNA表达的影响。结果显示:归参汤高剂量组可以显著增加免疫抑制小鼠血清中IL-2、TNF-α的含量(P<0.01),提高INF-γ的表达(P<0.05),降低IL-4的含量(P<0.05),升高T-bet(P<0.05),降低GATA-3的表达(P<0.05)。归参汤的免疫增强作用与升高血清中IL-2、TNF-α有关,且升高INF-γ/IL-4纠正免疫抑制小鼠Thl/Th2细胞因子的比例紊乱,其机制可能部分归因于对T-bet和GATA-3的mRNA表达的影响。     

丙氨酸-ESR剂量计用于测量产品箱内剂量分布的研究

本文用自制丙氨酸-ESR剂量计和西德辐射与环境研究中心(GSF)的丙氨酸-ESR剂量计,测量了北京市射线应用中心的~(60)Coγ辐射源(1.44×10~(16)Bq)辐照纸箱包装(913mm×555mm×460mm)产品箱时的吸收剂量分布,箱内模拟产品(报纸)的堆积密度为200ks/m~3,测量结果表明,产品箱內最大剂量D_(max)与最小剂量D_(min)之比分别为1.45(北京市辐射中心)和1.47(GSF),两者相差1.40％,在允许误差范围之内。     

Induction and assessment of morpho-biochemical mutants in Artemisia pallens Bess.

Artemisia pallens Bess. is a low volume and high value essential oil plant used in perfumery, cosmetic and flavouring industries. On account of the failure of conventional procedures to induce variability in species, mutation techniques have been tried in our experiments. Dry and viable seeds (moisture content 8%) of homozygous pure breeding lines were subjected to 150–500 Gy doses of gamma rays and 0.01–0.1% ethyl methane sulphonate (EMS) for 8 h. Desirable qualitative mutants were recovered from segregating M₂ generation (4,283 plants scored) raised as single plant progenies. The spectrum of morphological mutants included late and early flowering types; bushy and high yielding types; tall and more capitula-producing types and high oil and high davanone yielding types. These were raised through M₃ families to evaluate stability and transmission of mutant characters. As such out of 15 different types selected in M₂, only 11 types bred true to their characteristic variability. Based on their performance, the mutants were characterised depending upon their distinguishing features. Davanone, the main component of oil showed the maximum increase (64.22% against 54.64% in control) in mutant ‘S–5’ recovered from exposure with 250 Gy γ-rays. Mutant ‘E-6’ was economically most viable having increased oil biosynthesis (0.36% against 0.22% in control) and hence yields higher oil per unit area than the parental control (isolated from 0.05% EMS treatment).     

~(60)Coγ射线对甘薯黑斑病(Ceratocystis fimbriata)的抑制效果

用60~(Co)γ射线25—400krad的不同剂量辐照甘著黑斑病菌及带病甘薯(Ipomoeabatatas Lam.)薯块,发现200及400krad剂量的辐照对菌丝体和子囊孢子有一定的抑制作用,对分生孢子、厚垣孢子抑制作用甚微;200krad在20天内基本抑制薯块上病斑的发生和发展,薯块外观良好,接种发病率比对照低75％,病斑平均直径不及对照的五分之一;400krad的抑茵效果更高,但尚不能完全杀死病菌孢子,并对薯块外表有伤害,使其色泽变暗,贮藏后期易产生干腐。本试验表明,200krad剂量的辐照处理对甘著贮藏有实践意义。     

木材光引发自由基规律的研究

木材在~(60)Co γ射线辐射下引发g值为2.019、2.029、2.027、2.016、1.999等几种自由基。ESR主谱自由基浓度远大于机械自由基。并与树种、辐射剂量等有关。主谱自由基相对强度随剂量x按指数率Y=1-e~(-ax)上升,当剂量增加到100KGy时趋于饱和,根据福建21个常见树种测试有明显的规律性。     

富γ-氨基丁酸茶的药理功能研究进展

综述了富γ-氨基丁酸茶的药理功能和γ-氨基丁酸富集方法的研究进展,提出应进一步改进和完善富γ-氨基丁酸茶的加工工艺和我国富γ-氨基丁酸茶叶产品的广阔市场前景。     

IFN-γ对妊娠早期大鼠下丘脑中IL-18表达的影响

为探讨y干扰素(IFN-y)对妊娠早期大鼠下丘脑中白细胞介素-18(IL-18)表达及其对妊娠结局的影响,将妊娠9d的SD大鼠随机分为对照组、试验1组和试验2组,采用生殖道肌肉注射的方法,对照组大鼠生殖道肌肉注射生理盐水,试验组大鼠注射IFN-y,其中1 组剂量为2.5×104 U/只,2组剂量为7.5×104 U/只...     

富硒和γ-氨基丁酸萌芽糙薏米培养液组分的工艺优化

为提高糙薏米中γ-氨基丁酸的富集量,采用响应面试验法对糙薏米的培养液组分进行工艺优化。结果表明:培养液中Na2SeO3浓度以10mg/L为宜,其发芽率最高,达(90.33±1.73)%;在Na2SeO3浓度为10mg/L的培养液中辅以11mmol/L CaCl2、87.7μmol/L GA3和31.5mmol/L MSG进行萌芽处理时,萌芽糙薏米中总硒、有机硒和GABA含量分别达0.42mg/100g、0.37mg/100g和116.69mg/100g,是一种具高值化产品开发潜力的保健食品基料。     

~（60）Co γ射线不同剂量辐射甘蔗种芽试验初报

采用２０００γ、３５００γ、５０００γ、６５００γ、８０００γ５个等级的６０Ｃｏγ射线对甘蔗种芽进行电离辐射处理，观察对甘蔗生长发育的影响及苗期辐射形态变化，发现随着辐射剂量的增加出苗率明显下降，生长亦严重受阻，并发生矮化苗，茎色改变，心叶不抽出，株型变化等多种辐射形态变化。３５００γ以上的处理总变化数均在５０％以上，尤以５０００γ以上处理总变化率高，但差异不显著。初步认为蔗芽辐射育种的剂量范围为３５００－８０００γ，尤以３５００－６５００γ１为宜。     

Propagation in vitro of pear, Pyrus communis L., cultivars ‘William's Bon Chrétien’, ‘Packham's Triumph’ and ‘Beurré Bosc’

Shoot cultures were established from nodal explants of 3 pear cultivars by subculture on Murashige and Skoog medium containing mixtures of 3 cytokinins. Zeatin and 6·(γ,γ-dimethylallylamino)purine were both supplied at 10 μM and benzyladenine as follows: ‘William's Bon Chrétien’, 6 μM; ‘Packham's Triumph’, 8 μM; ‘Beurré Bosc’, 10 μM. Production of axillary shoots was greater by basal explants (4–6) comprising the remnants of the previous subculture than by apical explants (2–3) comprising the distal parts of extension shoots. Up to 80% of microcuttings of all cultivars formed roots in vitro with either γ-indolebutyric acid, 10 μM, or α-naphthaleneacetic acid, 10 μM, and approximately 50% of rooted microcuttings were successfully established as container-grown plants. Use of aseptic methods for producing own-rooted pears is discussed in relation to fruit growing and fruit improvement.