高效液相色谱法测定血浆或组织中吡喹酮,氯霉素,喹乙醇及利血平的浓度

本研究建立了测定吡喹酮、氯霉素、喹乙醇及利血平浓度的反相高效液相色谱分析方法.以ODS-C18化学键合相(5μm)作为固定相;乙醚萃取血浆中的吡喹酮,组织喹乙醇用乙酸乙酯-乙腈(3:2,V/V)抽提,虾肉氯霉素及组织利血平均用乙酸乙酯提取;甲醇-水为流动相;吡喹酮、氯霉素、喹乙醇及利血平进行紫外检测的波长分别是211、280、260及254nm,最低检出浓度分别是0.02μg/ml血浆、0.02μg/g虾肉、0.2μg/g组织及0.1μg/g组织,方法回收率分别是98.07±3.24、90.12±4.71、71.23±5.49及92.14±5.68％(平均值±标准差).这些方法简单、灵敏、专一,已用于测定猪静注及内服吡喹酮后的血浆药物浓度,对虾饲喂“虾病宝7号”(含氯霉素)后虾肉中氯霉素的残留,鸡内服中毒剂量喹乙醇的组织浓度,以及鸡内服利血平的组织浓度.     

Behaviour and reproductive performance of Nigerian native chickens subjected to different mating ratios for a short term

To date, there is no reported standard mating ratio for Nigerian native chickens despite their large population in the country. This study investigated the effect of mating ratio on behaviour, semen quality and level of stress. Sixty-three (9 cocks and 54 hens) sexually matured birds were randomly assigned to three mating ratios (MR) of 1 cock to 3 hens (1C:3H), 1 cock to 6 hens (1C:6H) and 1 cock to 9 hens (1C:9H), and each MR was replicated three times. Each cock spent 2 weeks in a particular MR before it was moved to another. By the 6th week, all cocks had experienced the three mating ratios. Behaviour of the hens and sexual behaviour of the cocks were observed in the morning and evening, respectively, for 6 weeks. At the end of 2nd week in each MR, semen samples were collected and evaluated for motility, morphology, liveability and concentration, and blood samples were also taken from the cocks to determine the heterophil/lymphocyte (H:L) ratio, an indicator of stress. There was also no significant effect of mating ratio on the fertility of eggs and hatchability of the chicks. The number of hen–hen aggression instances, frequency of visits to the nest box and duration of nesting was not influenced by the mating ratio. However, the duration of nest seeking was higher at 1C:3H and lower at 1C:9H. A mating ratio of 1C:9H is therefore recommended for farmers interested in raising Nigerian native chickens.     

无花果的桑天牛防治初步研究

桑天牛Apriona germari（Hope）在上海地区无花果果园中为害较严重,被害株率达89％;经2种农药的防治试验,均有较好的杀虫效果。     

中国斑目管蚜蝇属种类及一新种记述（双翅目：食蚜蝇科）

记录了中国斑目管蚜蝇属的１２种，其中１新种：八斑斑目管蚜蝇Ｌａｔｈｙｒｏｐｈｔｈａｌｍｕｓｏｃｔｏｐｕｎｃｔａｔｕｓｓｐ．ｎｏｖ．，１新记录种：石桓斑目管蚜蝇ＬａｔｈｙｒｏｐｈｔｈａｌｍｕｓｉｓｈｉｇａｋｉｅｎｓｉｓＳｈｉｒａｋｉ．给出了中国１２个种的检索表及其分布范围，模式标本保存于华南农大植保系。     

2甲4氯钠防除稻田扁杆镳草药效试验

采用不同剂量对比试验，研究了2甲4氯钠粉剂对稻田扁杆镳草的防除效果。结果表明：在水稻分蘖盛期，用2甲4氯钠粉剂2．25kg／hm^2进行茎叶喷雾，可有效防除杂草，且对水稻生长无不良影响。     

禾谷缢管蚜2种翅型生物学习性的比较研究

对禾谷缢管蚜有翅型与无翅型生物学习性比较研究，结果表明２种翅型在体重、胚胎数、蜜露排泄量、耐高温能力等方面均存在显著差异；２种翅型蚜，其体内胚胎数与体重之间均呈显著正相关。     

浙江悬钩子属植物的综合研究─—蓬的生物学特性

从形态学、解剖学、资源分布、营养生长节律，繁殖和种子生物学等方面对蓬作了探讨，并进行了初步的栽培试验和果实成分的分析及加工试验．结果表明：蓬　（ＲｕｂｕｓｈｉｒｓｕｔｕｓＴｈｕｎｂ．）分布广，资源丰富，适应性和抗逆性强，果实的食用价值较高，是浙江悬钩子属植物中具有引种、驯化、育种价值和开发利用前景的野生种之一．     

中国梧桐科植物花粉类型的研究

用光学显微镜和扫描电镜,对中国梧桐科22属59种的花粉进行形态观察,根据其花粉萌发孔的数目、形状、位置和花粉表面纹饰等,可以将它的花粉分为12个花粉类型:1.苹婆类型(Sterculia—type)、2.银叶树类型(Heritiera—type)、3.蛇婆子类型(Waltheria—type)、4.梭罗树类型(Reevesia—type)、5.滇桐类型(Craigia—type)、6.昂天莲类型(Ambroma—type)、7.刺果藤类型(Byttneria—type)、8.午时花类型(Pentapetes—type)、9.翅子树类型(Pterospermum—type)、10.山芝麻类型(Helicteres—type)、11.鹧鸪麻类型(Kleinhovia—type)、12.灾绳树类型(Eriola-ena—type)。本文从花粉学的观点,对中国梧桐树花粉类型的划分进行讨论。     

不同年代废弃的铅锌矿渣堆中重金属的动态变化

分别调查了堆积时间在10、20和近100a的铅锌矿渣堆,埋深在10、30、60cm的重金属含量变化情况。结果表明,由于雨水的淋溶作用和酸化,矿渣堆表层的重金属随堆积时间延长而减少,在100a的矿渣堆中,表层的铅含量仅有575.51mg·kg-1,但下层含量却达到5144.57mg·kg-1。在这些矿渣堆中,可溶性铅的含量也较高,最高达到了453.48mg·kg-1,而堆积20a和10a的矿渣中表层的重金属含量减少的程度较小,可溶性铅含量也相对小,最高含量分别为197.1mg·kg-1和50.11mg·kg-1。在堆积初期,表层的可溶态重金属含量较高,随时间的推移,下层含量逐渐增加,在某一个历史时期超过上层。