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1.
To investigate the effects and mechanisms of the benzimidazole carbamate and benmidine drugs on Cysticerci cellulosae and choose effective drugs on Cysticerci cellulosae, the membrane metabolism of Cysticerci cellulosae in vitro was tested after three kinds of drugs which were used respectively. The indexes included the contents of lipids, the contents of SA and the changes of the membrane fluidity. The results showed that oxfendazole could inhibit the membrane metabolism of immature and mature Cysticerci cellulosae in vitro, and albendazole only inhibited the mature one, while thibendimidine neither acted on the immature nor mature one. 相似文献
2.
3.
猪肺炎支原体及其生物学研究进展 总被引:10,自引:0,他引:10
猪肺炎支原体是引起猪支气管肺炎的主要病原 ,严重危害着养猪事业的发展。虽然猪肺炎支原体的结构简单 ,但由于它对营养要求较高 ,故培养难度大 ,也是人们不能对其深入研究的一个主要原因。近年来利用克隆技术 ,对猪肺炎支原体从基因水平上进行了多种研究 ,已经取得了一些可喜成果。文章从它的生物学特性、荚膜结构、膜蛋白研究以及基因水平的研究等方面进行了综合性论述 ,并简明地指出了目前研究中存在的问题以及未来展望 相似文献
4.
磷对奶牛红细胞膜脂质的影响 总被引:5,自引:0,他引:5
对15例临床上有明显低磷血症的奶牛血清磷、红细胞膜脂质成分进行了测定。结果表明,低磷血症奶牛血清磷、红细胞膜磷脂明显降低;红细胞膜胆固醇及膜胆固醇/磷脂摩尔比值(nch/npl)明显升高,与健康对照组牛相比差异极显著(P<0.01);直线相关分析表明,血清磷与红细胞膜磷脂之间呈极显著正相关(r=0.917,y=10.852x+3.196,P<0.01);与红细胞膜胆固醇以及nch/npl之间分别呈显著负相关(r=-0.940,y=2.850x-1.072,P<0.01;r=-0.920,y=1.968x-1.401,P<0.01)。此外,红细胞膜微粘度与膜磷脂之间呈显著负相关(r=-0.954,y=19.122x-4.384,P<0.01),而与膜胆固醇以及nch/npl之间呈显著正相关(r=0.988,y=0.927x+0.964,P<0.01;r=0.978,y=-0.293x+1.113,P<0.01)。结果表明,磷是红细胞膜脂质成分改变的先决因素,而后者又是红细胞膜流动性及膜结构、功能发生变化并导致膜损伤的一个重要因素。 相似文献
5.
本工作用光镜和电镜技术对全沟硬蜱的围食膜进行了初步观察研究。结果表明,饥饿幼虫、若虫、成虫以及吸血期雄性成虫不具围食膜。吸血期幼虫、若虫及雌性成虫最晚分别于吸血开始后20、20及18小时出现了单层结构的围食膜;缓慢吸血期围食膜的厚度持续增加,而在快速吸血期其厚度却会显著减小。饱血后发育期围食膜不断增厚,愈来愈拱曲,与肠壁的距离不断扩大,并形成多层结构;至少分别于饱血后12、25及11天,仍能在幼虫、若虫及雌性成虫肠腔观察到完好状态的围食膜。全沟硬蜱是我国北方地区多种血液传播病原体的重要媒介,其围食膜的发现及围食膜形态变化的初步描述,对进一步认识这些病原体在全沟硬蜱体内迁移扩散规律、发育动态以及向宿主的传播途径,可提供有益的启示。 相似文献
6.
棉花黄萎病菌毒素结合位点初探 总被引:6,自引:1,他引:5
以纯化的棉花黄萎病菌毒素(PLPC)免疫新西兰白兔制备了PLPC特异性抗血清。将PLPC(15μg·ml 1)用不同浓度的抗体(1~20μg·ml 1IgG)吸附后处理泗棉3号的切根苗,毒素所引起的症状都有不同程度的减轻。表明所制备的抗体在与毒素发生特异性免疫学反应的同时,可部分封闭毒素分子上与毒素受体结合的位点。利用竞争ELISA测定了泗棉3号幼苗子叶的质膜制剂与PLPC的结合活性。结果表明,质膜制剂与毒素结合后能部分阻断毒素与其抗体的免疫学反应,即质膜制剂中含有毒素的结合位点。分别用胰蛋白酶和煮沸处理质膜制剂后,质膜制剂对毒素与其抗体的反应的抑制作用消失,初步表明质膜制剂中与毒素结合的是蛋白质。 相似文献
7.
以苯—乙醇为夹带剂,研究超临界CO2流体不同处理压力、温度、时间对福建产杉木、马尾松、檫木木材渗透性的影响。结果表明,在35 MPa内,处理压力的增大有利于3种木材渗透性的提高;杉木在处理温度为40℃时,渗透性的变化率达到最大,为493%,马尾松和檫木在处理温度为45℃时,渗透性的变化率达到最大,分别为479%和349%;较佳的处理时间为30 min。 相似文献
8.
P. Gustin B. Urbain A. Delaunois K. Zeimes M. Ansay 《Veterinary research communications》1992,16(1):69-82
The right and left lungs of 5 healthy Minipigs and of 13 healthy Landrace piglets were isolated, perfused at constant pressure and maintained in an isogravimetric state under zone III conditions (pulmonary venous pressure>alveolar pressure). By applying the double, arterial and venous, occlusion technique, the total blood flow resistance (R
t) was partitioned into four components: arterial (R
a), pre-(R
a) and post-capillary (R
v) and venous (R
v). The capillary filtration coefficient (K
f,c) was evaluated by measuring the weight gained by the lungs when the arterial and venous pressures were suddenly increased. In the youngest Landrace piglets (5 weeks old), there was an uncontrolled vasoconstriction which sometimes prevented perfusion of the lungs and induced a large increase inR
t. These high values ofR
t were decreased by tolazoline administration. The values ofR
t recorded in older pigs (12–13 weeks old) were lower in Minipigs (33.66±3.77 cmH2O min L–1 per 100 g of lungs;n=5) than in Landrace piglets (55.20±6.18 cmH2O min L–1 per 100 g;n=5). This breed difference was due to the differences inR
a andR
v. The mean values ofK
f,c were 0.193±0.015 and 0.202±0.029 ml min (cmH2O)–1 per 100 g of the lungs in Minipigs and Landrace piglets respectively. All these parameters were stable for the 3 hours following the equilibrium period. It was concluded that: (1) There is an age-related maturation of the control of the vasomotor tone in porcine lungs. (2) Pulmonary microvascular haemodynamics are influenced by the breed of the pigs. (3) There was no difference in theK
f,c values between both the breeds. (4) A comparison of the values reported for dogs and rabbits with our data shows that the pre- and post-capillary resistances and, to a lesser extent, the arterial and venous resistances are relatively high in pigs. 相似文献
9.
Pushpanjali R.L. Prasad S.K. Singh S.B. Jadhao 《Pesticide biochemistry and physiology》2005,82(2):103-114
There is a lack of laboratory-based embryonic chicken toxicity studies with the ecologically relevant low dose/s of endosulfan that utilizes a more practical approach such as the chorioallontoic membrane (CAM) injection. In this investigation, 2μg AR grade α-endosulfan/egg (40% of LD50 for embryos) was injected through the CAM in 12-day-old chicken embryos and the activities of glucose-6-phosphatase (G6Pase, EC 3.1.3.9), fructose 1,6-diphosphatase (FDPase, EC 3.1.3.11), adenosine triphosphatase (ATPase, EC 3.6.1.3) and succinic dehydrogenase (SDH, EC 1.3.99.1) and DNA and RNA content in liver and brain tissues and acetyl cholinesterase (AChE, EC 3.1.1.7) in the latter were determined at 24, 48, and 72 h post-exposure. The wet weight of the embryos did not differ between groups. Following endosulfan exposure, except increase in the hepatic ATPase activity (P < 0.01), there was a significant decrease in the following parameters: G6Pase activity in both the liver and brain (P < 0.01), SDH activity in the brain (P < 0.01), brain overall DNA and RNA concentration (P < 0.05), brain AChE activity (P < 0.01). Exposure of 18-day-old embryos to 2-μg endosulfan for 24 h caused decrease (P < 0.01) in the lymphocyte count and IgG content. Histopathology of thymus and bursa of Fabricius revealed a reduction in the population of thymic follicles, smaller thymocytes with the clear vacuoles in cytoplasm and fewer bursocytes accompanied by infiltration of erythrocytes in lymphoid follicles of the endosulfan-treated embryos. It was inferred that in ovo injection of 0.041 μg/g egg weight of α-endosulfan suppress gluconeogenesis (main energy source in embryonic life), nerve transmission, and immunity. 相似文献
10.
The increased microvascular permeability appears mainly in venule during inflammation, shock, and burns. Endothelial cells play an important role in venule permeability enhancement. There are two kinds of pathway for macromolecule extravasation. One is paracellular pathway and another is transcellular pathway, which are related to the formation of endothelial gap or transcellular openings seperately. The alteration of intercellular related protein, such as occludin, claudin, zona occludens (ZO), junctional adhesion molecule (JAM), VE cadherin, catenin, integrin, etc, and the alteration of endothelial cytoskeleton, such as rearrangement of actin filament, formation of stress fiber and focal adhesion, etc, involve in the pathogenesis of increased microvascular permeability. 相似文献