母猪不孕症的针灸治疗

针灸治病是通过刺激腧穴,疏通经络,以调节机体的阴阳气血、脏腑功能、体表肌肤及筋骨百骸得以濡养等,使其发挥正常的生理功能,从而达到治疗疾病的目的。主要对性成熟后不孕母猪进行针灸治疗,结果其机体恢复正常,且正常受孕而产仔。表明针灸治疗对母猪不孕症的治疗具有重要的指导和参考意义。     

Equine chronic diarrhea: Traditional Chinese veterinary medicine review

Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included.     

CAN SONOGRAPHIC FINDINGS PREDICT THE RESULTS OF LIVER ASPIRATES IN DOGS WITH SUSPECTED LIVER DISEASE?

While abdominal ultrasound and ultrasound-guided fine-needle aspiration cytology are often combined to help determine the type of liver disease in dogs, little is known about the relationship that may exist between the results of these tests. We hypothesized that specific sonographic findings, or combinations of findings, may predict results of liver ultrasound-guided fine-needle aspiration cytology. Hepatic and extrahepatic sonographic findings were recorded prospectively using a standardized form in 70 dogs with clinically suspected liver disease and in which liver ultrasound-guided fine-needle aspiration cytology was performed. The predictive value of sonographic findings in regard to the category of cytology results was assessed with stepwise logistic regression analysis. Sonographic detection of a hepatic mass (≥3 cm; risk ratio [RR] 3.83, 95% Wald confidence intervals [95% CI] 2.42–3.93, P =0.0036), ascites (RR 3.82, 95% CI 1.94–4.28, P =0.0044), abnormal hepatic lymph node(s) (RR 3.01, 95% CI 1.22–4.88, P =0.0262), and abnormal spleen (RR 3.26, 95% CI 1.20–3.85, P =0.0274) were the most predictive of liver neoplasia on cytology. Conversely, sonographic detection of hepatic nodules (<3 cm; RR 1.97, 95% CI 0.95–2.96, P =0.0666) was most predictive of vacuolar hepatopathy on cytology. In dogs with suspected liver disease, several sonographic findings, alone or combined, are thus predictive of liver ultrasound-guided fine-needle aspiration cytology results. In the light of the fact that ultrasound-guided fine-needle aspiration cytology of the liver has limitations, these predictabilities could influence the selection of diagnostic tests to reach a reliable diagnosis.     

OSTEOSARCOMA OF THE PATELLA WITH PULMONARY METASTASES IN A DOG

A 6-year-old neutered male Rottweiler was examined for a progressive right pelvic limb lameness. In radiographs of the right stifle, there was an osteolytic lesion with irregular new bone formation along the cranial aspect of the patella consistent with an aggressive bone lesion. In thoracic radiographs, there were multiple soft tissue nodular opacities throughout the lung fields, consistent with pulmonary metastases. Microscopically, fine needle aspirate samples from the right patella contained pleomorphic spindle cells with cytologic features of osteosarcoma. The presence of pulmonary metastases at the time of initial diagnosis in the dog described herein suggests that osteosarcoma of the patella has the potential for similar aggressive biologic behavior as that seen in dogs with appendicular osteosarcoma.     

CORRELATION OF ULTRASOUND FINDINGS,LIVER AND SPLEEN CYTOLOGY,AND PROGNOSIS IN THE CLINICAL STAGING OF HIGH METASTATIC RISK CANINE MAST CELL TUMORS

Cytologic sampling of the ultrasonographically normal spleen and liver is not implemented routinely in the clinical staging of canine cutaneous mast cell tumors and normal ultrasound findings are often accepted as sufficient evidence for ruling out splenic or liver metastasis. Our objective was to define the specificity and sensitivity of ultrasound findings for diagnosis of mast cell infiltration when verified with cytologic evaluation, and to define the prognostic role of cytologic evaluation of liver and splenic aspirates. Dogs with a diagnosis of clinically aggressive grade II, or grade III mast cell tumor treated with a combination vinblastine/CCNU chemotherapy protocol, were selected retrospectively based on availability of cytologic evaluation of spleen plus or minus liver for staging. Out of 19 dogs, 10 dogs had a grade II tumor and nine a grade III tumor. Seven dogs had mast cell infiltration of the spleen, liver, or both. The sensitivity of ultrasound for detecting mast cell infiltration was 43% for the spleen and 0% for the liver. Dogs with positive cytologic evidence of mast cell infiltration to spleen, liver, or both had significantly shorter survival (100 vs. 291 days) than dogs without evidence of mast cell infiltration (P<0.0001). Routine splenic aspiration should be performed regardless of ultrasonographic appearance in dogs with a clinically aggressive mast cell tumor.     

The incidence and significance of excess acupuncture channel imbalance in the equine sport horse purchase examination, 1999–2004

Prepurchase examinations are commonly used in the evaluation of sport horses. No data have been published concerning the use of traditional Chinese veterinary medical (TCVM) channel palpation with respect to the musculoskeletal status of sport horses for sale. The objective of the study is to summarize the results of channel evaluation when integrated with a Western examination in a series of prepurchase examinations. The prepurchase examination records of 235 sport horses were reviewed. The examined horses were presented as serviceable for the intended use. The examinations were performed by a single observer. The TCVM palpation examination and Western examinations, which often included radiography, infrared thermometry, diagnostic ultrasound, fiberoptic endoscopy, and diagnostic blood screens, were used to further evaluate the musculoskeletal system. The data were used to define the relationship between soundness, lameness, channel imbalance, and sale outcome. Over 6 years, 235 horses were examined. One hundred fifty-six (66%) were sound at their working gaits, and 79 (34%) had observable lameness. Two thirds of lame horses had palpable channel imbalance. Only a quarter of sound horses presented with palpable channel imbalance. Sound horses with no channel imbalance, that is, level channels, were most frequently sold, and lame horses with channel imbalance most frequently were not sold. Significantly more sound horses with level channels were sold as compared with sale outcome of sound horses with unbalanced channels. In conclusion, the TCVM channel examination is a useful component in the integrated prepurchase examination of sport horses.     

徒手克隆工具自制及改进

徒手克隆技术(handmade somatic cell cloning,HMC)解决了由于昂贵设备对核移植技术应用的制约问题。通过徒手克隆刀的自制,不但进一步节省了成本,且更能符合使用者的习惯,提高总的切割效率。试验结果表明,利用自制克隆刀进行半卵法去核,可以达到每小时切割496枚卵母细胞的切割速度和88.14%的存活率;自制聚集针具有制作简单,可以打磨、效果好等特点,可以进一步简化和降低徒手克隆技术的成本,提高克隆效率。